US5580859A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Delivery of exogenous DNA sequences in a mammal
Assignee: Wisconsin Alumni Research Foundation
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Filing: 1994-03-18
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Granted: 1996-12-03
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Expires: 2014-03-18
Abstract
Abstract Polynucleotide sequences, comprising DNA and RNA molecules can be directly administered, for example by injection, to tissues, such as muscle, and expressed as a protein, polypeptide or polypeptide. The polynucleotides can be contained within liposomes or the polynucleotides can free from association with transfection-facilitating proteins, viral particles, liposomal formulations, charged lipids and calcium phosphate precipitating agents.
Inventors
- 👤 Philip L. Felgner
- 👤 Jon A. Wolff
- 👤 Gary H. Rhodes
- 👤 Robert W. Malone
- 👤 Dennis A. Carson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 17:54
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/005; A; A61; A61K; A61K48/00; A61K9/00; A61K9/10; A61K9/127; A61K9/1271; A61K9/1272; C07K14/435; C07K14/575; C07K14/61; C12; C12N; C12N15/00; C12N15/09; C12N15/87; C12N9/00; C12N9/0004; C12N9/0069; C12N9/10; C12N9/1025; C12N9/1029; C12N9/1033; C12N9/12; C12N9/1241; C12N9/1247; C12N9/14; C12N9/78; C12Y; C12Y113/00; C12Y113/12; C12Y113/12007; C12N2740/00; C12N2740/00011; C12N2740/10011; C12N2740/16011; C12N2740/16111; C12N2740/16122
Claims Analysis
Total Claims: 21
Claim 1
Independent Claim
67 words
A method for delivering a desired physiologically active protein, polypeptide or peptide to a mammal, comprising injecting into the muscle of the mammal a non-infectious, non-integrating DNA sequence encoding said protein, polypeptide or peptide operably linked to a promoter, wherein said DNA sequence is free from association with transfection-facilitating proteins, viral particles, liposomal formulations, charged lipids and calcium phosphate precipitating agents, whereby said DNA sequence is expressed.
Claim 2
Independent Claim
67 words
A method for delivering a desired physiologically active protein, polypeptide or peptide to a mammal, comprising injecting into the muscle of the mammal a non-infectious, non-integrating DNA sequence encoding said protein, polypeptide or peptide operably linked to a promoter, wherein said DNA sequence is free from association with transfection-facilitating proteins, viral particles, liposomal formulations, charged lipids and calcium phosphate precipitating agents, whereby said DNA sequence is expressed.
Claim 3
Dependent Claim
23 words
The method of claim 1, wherein said DNA is free from a delivery vehicle to facilitate entry of the DNA into the cell.
Claim 4
Dependent Claim
11 words
The method of claim 1, wherein said DNA comprises a plasmid.
Claim 5
Dependent Claim
16 words
The method of claim 1, wherein said promoter is a cell specific or tissue specific promoter.
Claim 6
Dependent Claim
11 words
The method of claim 1, wherein said muscle is skeletal muscle.
Claim 7
Dependent Claim
15 words
The method of claim 1, wherein said injection comprises impressing said DNA through the skin.
Claim 8
Dependent Claim
16 words
The method of claim 1, wherein said injection comprises injection of said DNA through a needle.
Claim 9
Dependent Claim
31 words
The method of claim 1, wherein said injection comprises injecting said DNA into the interstitial space of said muscle resulting in transfection of said DNA into muscle cells of said mammal.
Claim 10
Dependent Claim
11 words
The method of claim 1, wherein said mammal is a human.
Claim 11
Dependent Claim
32 words
The method of claim 1, wherein said DNA is operably linked to a DNA sequence encoding a signal peptide wherein the signal peptide directs the secretion of the protein, polypeptide or peptide.
Claim 12
Dependent Claim
16 words
The method of claim 1, where the expression of said protein, polypeptide or peptide is transitory.
Claim 13
Dependent Claim
13 words
The method of claim 1, wherein said protein, polypeptide or peptide is therapeutic.
Claim 14
Dependent Claim
13 words
The method of claim 1, wherein said protein, polypeptide or peptide is prophylactic.
Claim 15
Dependent Claim
14 words
The method of claim 1, wherein said protein, polypeptide or peptide is an enzyme.
Claim 16
Dependent Claim
14 words
The method of claim 1, wherein said protein, polypeptide or peptide is a hormone.
Claim 17
Dependent Claim
14 words
The method of claim 1, wherein said protein, polypeptide or peptide is a lymphokine.
Claim 18
Dependent Claim
16 words
The method of claim 1, wherein said protein, polypeptide or peptide ms a cell surface receptor.
Claim 19
Dependent Claim
15 words
The method of claim 1, wherein said protein, polypeptide or peptide is a growth factor.
Claim 20
Dependent Claim
18 words
The method of claim 1, wherein said protein polypeptide or peptide ms produced for at least one month.
Claim 21
Dependent Claim
19 words
The method of claim 1, wherein said protein, polypeptide or peptide ms produced for less than about 10 days.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1999-12-03 | 2000-06-03 | 2000-12-03 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2003-12-03 | 2004-06-03 | 2004-12-03 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2007-12-03 | 2008-06-03 | 2008-12-03 | $7700.00 | Window Closed / Lapsed |