US5580971A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Fungal detection system based on rRNA probes
Assignee: Resonac Corp
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Filing: 1995-01-26
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Granted: 1996-12-03
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Expires: 2015-01-26
Abstract
Abstract The invention includes methods for the detection of a particular genus or species of fungus in a biological sample. Some of these methods make use of a solid support-polynucleotide structure. This structure includes a solid support having immobilized thereto a polynucleotide probe that is complementary to a sequence of ribosomal RNA (rRNA) specific to the particular species of fungus. An rRNA sequence from the particular species of fungus is hybridized to the first probe, and a second polynucleotide probe is also hybridized to the rRNA.
Inventors
- 👤 Masato Mitsuhashi
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 06:02
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6876; C12Q1/6888; C12Q1/6895
Claims Analysis
Total Claims: 3
Claim 1
Independent Claim
122 words
A combination of isolated polynucleotides having about the same at selected ionic strength with their complementary polynucleotides, said combination a plurality of different specific polynucleotides, each of said different specific polynucleotides having a sequence selected from the group consisting of SEQ ID NO:81, SEQ ID NO:104, SEQ ID NO:131, SEQ ID NO:154, SEQ ID NO:176, SEQ ID NO:199, SEQ ID NO:267, SEQ ID NO:290, SEQ ID NO:312, SEQ ID NO:335, SEQ ID NO:364, SEQ ID NO:391, and a sequence complementary to any of the foregoing; and at least one common polynucleotide having a sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:21, SEQ ID NO:41, and SEQ ID NO:61, and a sequence complementary to any of the foregoing.
Claim 2
Dependent Claim
122 words
A combination of isolated polynucleotides having about the same at selected ionic strength with their complementary polynucleotides, said combination a plurality of different specific polynucleotides, each of said different specific polynucleotides having a sequence selected from the group consisting of SEQ ID NO:81, SEQ ID NO:104, SEQ ID NO:131, SEQ ID NO:154, SEQ ID NO:176, SEQ ID NO:199, SEQ ID NO:267, SEQ ID NO:290, SEQ ID NO:312, SEQ ID NO:335, SEQ ID NO:364, SEQ ID NO:391, and a sequence complementary to any of the foregoing; and at least one common polynucleotide having a sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:21, SEQ ID NO:41, and SEQ ID NO:61, and a sequence complementary to any of the foregoing.
Claim 3
Dependent Claim
25 words
The combination of isolated polynucleotides according to claim 1 wherein said T m at selected ionic strengths with their complementary polynucleotides is 54° C.±1° C.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1999-12-03 | 2000-06-03 | 2000-12-03 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2003-12-03 | 2004-06-03 | 2004-12-03 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2007-12-03 | 2008-06-03 | 2008-12-03 | $7700.00 | Window Closed / Lapsed |