US5597696A
Expired / Lapsed
US
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Covalent cyanine dye oligonucleotide conjugates
Assignee: Becton Dickinson and Co
•
Filing: 1994-07-18
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Granted: 1997-01-28
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Expires: 2014-07-18
Abstract
Abstract The present invention relates to conjugates of a cyanine dye and an oligonucleotide. When these conjugates hybridize or bind to a target, a detectable increase in fluorescence intensity or change in fluorescence polarization is observed.
Inventors
- 👤 C. Preston Linn
- 👤 J. Bruce Pitner
- 👤 Pat D. Mize
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 23:36
CPC Classifications
C; C07; C07H; C07H21/00; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6813; C12Q1/6816
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
93 words
A compound of the formula ##STR2## wherein X is O, S, Se, N-alkyl having from 1-6 carbons or C(CH 3 ) n ; R 1 is alkyl having from 1-6 carbons; R 2 is alkyl having from 1-6 carbons; R 3 is fused benzene, alkyl having from 1-6 carbons, methoxy or is absent, R 4 is alkyl having from 1-6 carbons, methoxy or is absent; Z is an oligonucleotide having from 8 to 50 bases; n is zero or an integer from 1-6; and n 1 is an integer from 2 to 10.
Claim 2
Dependent Claim
93 words
A compound of the formula ##STR2## wherein X is O, S, Se, N-alkyl having from 1-6 carbons or C(CH 3 ) n ; R 1 is alkyl having from 1-6 carbons; R 2 is alkyl having from 1-6 carbons; R 3 is fused benzene, alkyl having from 1-6 carbons, methoxy or is absent, R 4 is alkyl having from 1-6 carbons, methoxy or is absent; Z is an oligonucleotide having from 8 to 50 bases; n is zero or an integer from 1-6; and n 1 is an integer from 2 to 10.
Claim 3
Dependent Claim
9 words
The compound of claim 1 wherein X is S.
Claim 4
Dependent Claim
10 words
The compound of claim 2 wherein n 1 is 3.
Claim 5
Dependent Claim
10 words
The compound of claim 2 wherein n 1 is 6.
Claim 6
Dependent Claim
9 words
The compound of claim 1 wherein X is O.
Claim 7
Dependent Claim
10 words
The compound of claim 5 wherein n 1 is 3.
Claim 8
Dependent Claim
10 words
The compound of claim 5 wherein n 1 is 6.
Claim 9
Dependent Claim
9 words
The compound of claim 1 wherein X is Se.
Claim 10
Dependent Claim
10 words
The compound of claim 8 wherein n 1 is 3.
Claim 11
Dependent Claim
10 words
The compound of claim 8 wherein n 1 is 6.
References Cited (Backward Citations)
US4883867A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2000-01-28 | 2000-07-29 | 2001-01-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2004-01-28 | 2004-07-29 | 2005-01-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2008-01-28 | 2008-07-29 | 2009-01-28 | $7700.00 | Window Closed / Lapsed |