US5656470A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Recombinant mycobacterial seryl-tRNA synthetase genes, tester strains and assays
Assignee: Cubist Pharmaceuticals LLC
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Filing: 1994-09-13
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Granted: 1997-08-12
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Expires: 2014-09-13
Abstract
Abstract Isolated and/or recombinant nucleic acids encoding mycobacterial seryl-tRNA synthetase have been characterized. Recombinant DNA constructs and vectors having a sequence which encodes mycobacterial seryl-tRNA synthetase have been made, and can be used for the construction of tester strains as well as for the production of isolated and/or recombinant seryl-tRNA synthetases. These enzymes or portions thereof are useful in the biochemical separation of serine and quantification of serine or ATP, and for producing antibodies useful in the purification and study of the enzyme, for example. Host cells and methods useful for producing recombinant mycobacterial seryl-tRNA synthetases are described, as are tester strains, which are cells engineered to rely on the function of the tRNA synthetase encoded by an introduced cloned gene. Tester strains can be used to identify inhibitors of the essential tRNA synthetase enzyme encoded by the introduced cloned gene, and thus provide a means to assess the antimicrobial effect and specificity of the inhibitor without employing slow-growing, pathogenic strains of mycobacteria, such as Mycobacterium tuberculosis.
Inventors
- 👤 Susan A. Martinis
- 👤 Jiansu Zhang
- 👤 Paul R. Schimmel
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 06:45
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/93
Claims Analysis
Total Claims: 33
Claim 1
Independent Claim
14 words
An isolated, recombinant nucleic acid which encodes a seryl-tRNA synthetase of the genus Mycobacterium.
Claim 2
Dependent Claim
14 words
An isolated, recombinant nucleic acid which encodes a seryl-tRNA synthetase of the genus Mycobacterium.
Claim 3
Dependent Claim
29 words
An essentially pure nucleic acid which hybridizes under stringent temperature conditions to DNA having the sequence in SEQ ID NO:3 and encodes a seryl-tRNA synthetase of the genus Mycobacterium.
Claim 4
Dependent Claim
29 words
An essentially pure nucleic acid which hybridizes under stringent temperature conditions to DNA having the sequence in SEQ ID NO:3 and encodes a seryl-tRNA synthetase of the genus Mycobacterium.
Claim 5
Dependent Claim
13 words
An isolated, recombinant nucleic acid which encodes a seryl-tRNA synthetase of Mycobacterium tuberculosis.
Claim 6
Dependent Claim
13 words
An isolated, recombinant nucleic acid which encodes a seryl-tRNA synthetase of Mycobacterium tuberculosis.
Claim 7
Dependent Claim
19 words
An essentially pure nucleic acid of claim 2 wherein the seryl-tRNA synthetase is a seryl-tRNA synthetase of Mycobacterium tuberculosis.
Claim 8
Dependent Claim
15 words
An essentially pure nucleic acid which encodes the amino acid sequence in SEQ ID NO:4.
Claim 9
Dependent Claim
15 words
An essentially pure nucleic acid which encodes the amino acid sequence in SEQ ID NO:4.
Claim 10
Dependent Claim
31 words
A recombinant nucleic acid vector comprising a nucleic acid which encodes a mycobacterial seryl-tRNA synthetase and which hybridizes under stringent temperature conditions to DNA having the sequence in SEQ ID NO:3.
Claim 11
Dependent Claim
31 words
A recombinant nucleic acid vector comprising a nucleic acid which encodes a mycobacterial seryl-tRNA synthetase and which hybridizes under stringent temperature conditions to DNA having the sequence in SEQ ID NO:3.
Claim 12
Dependent Claim
13 words
A recombinant DNA vector which contains DNA which encodes a mycobacterial seryl-tRNA synthetase.
Claim 13
Dependent Claim
13 words
A recombinant DNA vector which contains DNA which encodes a mycobacterial seryl-tRNA synthetase.
Claim 14
Dependent Claim
19 words
A recombinant nucleic acid vector of claim 6 wherein the seryl-tRNA synthetase is a seryl-tRNA synthetase of Mycobacterium tuberculosis.
Claim 15
Dependent Claim
14 words
A recombinant DNA vector comprising DNA which encodes a seryl-tRNA synthetase of Mycobacterium tuberculosis.
Claim 16
Dependent Claim
14 words
A recombinant DNA vector comprising DNA which encodes a seryl-tRNA synthetase of Mycobacterium tuberculosis.
Claim 17
Dependent Claim
18 words
A host cell which contains a recombinant mycobacterial seryl-tRNA synthetase gene which can express a mycobacterial seryl-tRNA synthetase.
Claim 18
Dependent Claim
18 words
A host cell which contains a recombinant mycobacterial seryl-tRNA synthetase gene which can express a mycobacterial seryl-tRNA synthetase.
Claim 19
Dependent Claim
21 words
A host cell of claim 10 in which the recombinant mycobacterial seryl-tRNA synthetase gene can express a Mycobacterium tuberculosis seryl-tRNA synthetase.
Claim 20
Dependent Claim
53 words
An expression vector comprising a nucleic acid encoding a fusion protein comprising a mycobacterial seryl-tRNA synthetase, wherein said nucleic acid comprises a coding sequence for mycobacterial seryl-tRNA synthetase, wherein the coding sequence is under the control of transcription signals and is linked to appropriate translation signals for expression in a suitable host cell.
Claim 21
Dependent Claim
53 words
An expression vector comprising a nucleic acid encoding a fusion protein comprising a mycobacterial seryl-tRNA synthetase, wherein said nucleic acid comprises a coding sequence for mycobacterial seryl-tRNA synthetase, wherein the coding sequence is under the control of transcription signals and is linked to appropriate translation signals for expression in a suitable host cell.
Claim 22
Independent Claim
75 words
A method for producing active mycobacterial seryl-tRNA synthetase comprising the following steps: a) constructing a recombinant nucleic acid vector comprising a coding sequence for mycobacterial seryl-tRNA synthetase wherein the coding sequence is under the control of transcription signals and is linked to appropriate translation signals; b) introducing the vector into suitable host cells which support the replication of the vector; and c) maintaining the host cells under conditions in which mycobacterial seryl-tRNA synthetase is expressed.
Claim 23
Independent Claim
75 words
A method for producing active mycobacterial seryl-tRNA synthetase comprising the following steps: a) constructing a recombinant nucleic acid vector comprising a coding sequence for mycobacterial seryl-tRNA synthetase wherein the coding sequence is under the control of transcription signals and is linked to appropriate translation signals; b) introducing the vector into suitable host cells which support the replication of the vector; and c) maintaining the host cells under conditions in which mycobacterial seryl-tRNA synthetase is expressed.
Claim 24
Dependent Claim
35 words
An isolated nucleic acid comprising a nucleic acid having a sequence complementary to a DNA strand having the sequence in SEQ ID NO:3 or to an RNA counterpart of the sequence in SEQ ID NO:3.
Claim 25
Dependent Claim
35 words
An isolated nucleic acid comprising a nucleic acid having a sequence complementary to a DNA strand having the sequence in SEQ ID NO:3 or to an RNA counterpart of the sequence in SEQ ID NO:3.
Claim 26
Independent Claim
42 words
A method for producing a mycobacterial seryl-tRNA synthetase comprising maintaining a host cell comprising a recombinant nucleic acid encoding a mycobacterial seryl-tRNA synthetase under conditions suitable for expression of the nucleic acid, whereby a mycobacterial seryl-tRNA synthetase is expressed and thereby produced.
Claim 27
Independent Claim
42 words
A method for producing a mycobacterial seryl-tRNA synthetase comprising maintaining a host cell comprising a recombinant nucleic acid encoding a mycobacterial seryl-tRNA synthetase under conditions suitable for expression of the nucleic acid, whereby a mycobacterial seryl-tRNA synthetase is expressed and thereby produced.
Claim 28
Dependent Claim
42 words
An isolated nucleic acid, wherein said nucleic acid encodes a protein comprising a seryl-tRNA synthetase which is encoded by pJZSer4 as deposited under ATCC Accession No. 98055 or a derivative of pJZSer4 in which G at position 1 is altered to A.
Claim 29
Dependent Claim
42 words
An isolated nucleic acid, wherein said nucleic acid encodes a protein comprising a seryl-tRNA synthetase which is encoded by pJZSer4 as deposited under ATCC Accession No. 98055 or a derivative of pJZSer4 in which G at position 1 is altered to A.
Claim 30
Dependent Claim
14 words
The isolated nucleic acid of claim 16, wherein the protein is a fusion protein.
Claim 31
Dependent Claim
11 words
The isolated nucleic acid of claim 16, which is essentially pure.
Claim 32
Dependent Claim
46 words
A host cell comprising a recombinant nucleic acid, wherein said nucleic acid encodes a protein comprising a seryl-tRNA synthetase which is encoded by pJZSer4 as deposited under ATCC Accession No. 98055 or a derivative of pJZSer4 in which G at position 1 is altered to A.
Claim 33
Dependent Claim
46 words
A host cell comprising a recombinant nucleic acid, wherein said nucleic acid encodes a protein comprising a seryl-tRNA synthetase which is encoded by pJZSer4 as deposited under ATCC Accession No. 98055 or a derivative of pJZSer4 in which G at position 1 is altered to A.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2000-08-11 | 2001-02-10 | 2001-08-12 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2004-08-11 | 2005-02-10 | 2005-08-12 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2008-08-11 | 2009-02-10 | 2009-08-12 | $7700.00 | Window Closed / Lapsed |