US5674848A
Expired / Lapsed
US
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Bioreactor compositions with enhanced cell binding
Assignee: University of California
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Filing: 1994-08-03
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Granted: 1997-10-07
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Expires: 2014-08-03
Abstract
Abstract Compositions of the invention include packing materials having compounds thereon with enhanced cell binding with respect to collagen. These packing materials are useful for in vitro uses such as for cell culture in bioreactors. Suitable compounds with enhanced cell binding include synthetic peptides that mimic the conformation necessary for recognition and docking of collagen binding species (such as cell surface receptors for collagen and fibronectin) and have the amino acid residues -Ile-Ala- folded in a beta -bend.
Inventors
- 👤 Rajendra S. Bhatnagar
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 05:02
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/0068; A; A61; A61L; A61L27/00; A61L27/28; A61L27/34; C07; C07K; C07K14/00; C07K14/435; C07K14/78; A61K; A61K38/00; A61L2430/00; A61L2430/02; C12N2533/00; C12N2533/30; C12N2533/50; C12N2533/54
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
117 words
A packing material useful for bioreactor cell culture comprising: a matrix formed of a biomaterial and a peptide carried by the matrix, the peptide having enhanced cell binding with respect to collagen, the peptide having a domain that mimics collagen binding to cells, said domain including at least -Ile-Ala- folded in a β-bend at physiologic conditions, the peptide selected from the group consisting of Gly-Thr-Pro-Gly-Pro-Gln-Gly-Ile-Ala-Gly-Gln-Arg-Gly-Val-Val (SEQ ID NO:1), Gly-Pro-Gln-Gly-Ile-Ala-Gly-Gln-Arg (SEQ ID NO:2), Gln-Gly-Ile-Ala-Gly-Gln (SEQ ID NO:3), Gln-Gly-Ile-Ala-Gly-Gln-Arg (SEQ ID NO:4), Phe-Gly-Ile-Ala-Gly-Phe (SEQ ID NO:5), Gly-Ile-Ala-Gly-Gln (SEQ ID NO:6), Gln-Gly-Ala-Ile-Ala-Gln (SEQ ID NO:7), Phe-Gly-Ile-Ala-Gly-Phe (SEQ ID NO:9), Cys-Gly-Ile-Ala-Gly-Cys (SEQ ID NO:10), Glu-Gly-Ile-Ala-Gly-Lys (SEQ ID NO:11), NAc-Ile-Ala-Ala (SEQ ID NO:12), Ile-Ala-βAla (SEQ ID NO:13), and NAc-Ile-Ala-N-Me (SEQ ID NO:14).
Claim 2
Dependent Claim
117 words
A packing material useful for bioreactor cell culture comprising: a matrix formed of a biomaterial and a peptide carried by the matrix, the peptide having enhanced cell binding with respect to collagen, the peptide having a domain that mimics collagen binding to cells, said domain including at least -Ile-Ala- folded in a β-bend at physiologic conditions, the peptide selected from the group consisting of Gly-Thr-Pro-Gly-Pro-Gln-Gly-Ile-Ala-Gly-Gln-Arg-Gly-Val-Val (SEQ ID NO:1), Gly-Pro-Gln-Gly-Ile-Ala-Gly-Gln-Arg (SEQ ID NO:2), Gln-Gly-Ile-Ala-Gly-Gln (SEQ ID NO:3), Gln-Gly-Ile-Ala-Gly-Gln-Arg (SEQ ID NO:4), Phe-Gly-Ile-Ala-Gly-Phe (SEQ ID NO:5), Gly-Ile-Ala-Gly-Gln (SEQ ID NO:6), Gln-Gly-Ala-Ile-Ala-Gln (SEQ ID NO:7), Phe-Gly-Ile-Ala-Gly-Phe (SEQ ID NO:9), Cys-Gly-Ile-Ala-Gly-Cys (SEQ ID NO:10), Glu-Gly-Ile-Ala-Gly-Lys (SEQ ID NO:11), NAc-Ile-Ala-Ala (SEQ ID NO:12), Ile-Ala-βAla (SEQ ID NO:13), and NAc-Ile-Ala-N-Me (SEQ ID NO:14).
Claim 3
Dependent Claim
24 words
The packing material useful for bioreactor cell culture as in claim 1 wherein the peptide is in an amount effective to promote cell attachment.
Claim 4
Dependent Claim
18 words
The packing material useful for bioreactor cell culture as in claim 1 wherein the matrix includes a hydrogel.
Claim 5
Dependent Claim
22 words
The packing material useful for bioreactor cell culture as in claim 1 wherein the matrix is in the form of polymer fibers.
Claim 6
Dependent Claim
21 words
The packing material useful for bioreactor cell culture as in claim 1 wherein the peptide is covalently bound to the matrix.
Claim 7
Dependent Claim
26 words
The packing material useful for bioreactor cell culture as in claim 5 wherein the matrix is in the form of fibers with interstices formed between fibers.
Claim 8
Dependent Claim
21 words
The packing material useful for bioreactor cell culture as in claim 5 wherein the matrix is in the form of beads.
Claim 9
Dependent Claim
30 words
The packing material useful for bioreactor cell culture as in claim 1 wherein the peptide is in an amount effective to promote cell attachment and the matrix includes a hydrogel.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2000-10-06 | 2001-04-07 | 2001-10-07 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2004-10-06 | 2005-04-07 | 2005-10-07 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2008-10-06 | 2009-04-07 | 2009-10-07 | $7700.00 | Window Closed / Lapsed |