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US5728822A
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Quaternary amine surfactants and methods of using same in isolation of RNA
Assignee: Qiagen NV
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Filing: 1995-09-07
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Granted: 1998-03-17
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Expires: 2015-09-07
Abstract
Abstract The present invention discloses a novel method for isolating RNA from biological samples, most particularly blood, using selected quaternary amine surfactants. The RNA is isolated quickly and in sufficient quantity and quality to permit analysis by methods including reverse transcriptase and polymerase chain reaction.
Inventors
- 👤 Donald E. Macfarlane
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 11:51
CPC Classifications
C; C07; C07C; C07C233/00; C07C233/89; C07C211/00; C07C211/62; C07C211/63; C07C211/64; C12; C12N; C12N15/00; C12N15/09; C12N15/10; C12N15/1003
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
59 words
A quaternary amine surfactant useful for isolating nucleic acid from a biological sample having the formula ##STR2## wherein R1 through R4 are independently selected from an alkyl chain containing from 1 to 20 carbons, and an aryl group containing from 6 to 26 carbons and X - is selected from the group consisting of oxalate, malonate, succinate and citrate.
Claim 2
Dependent Claim
59 words
A quaternary amine surfactant useful for isolating nucleic acid from a biological sample having the formula ##STR2## wherein R1 through R4 are independently selected from an alkyl chain containing from 1 to 20 carbons, and an aryl group containing from 6 to 26 carbons and X - is selected from the group consisting of oxalate, malonate, succinate and citrate.
Claim 3
Dependent Claim
52 words
The surfactant according to claim 1 wherein the quaternary amine is an alkylbenzyldimethylammonium, wherein R1 and R2 are alkyl groups of 1 carbon in length, R3 is an aryl group of 6 carbons, and R4 is an alkyl selected from the group consisting of 12, 14, 16, or 18 carbons in length.
Claim 4
Dependent Claim
67 words
A quaternary amine surfactant useful for isolating nucleic acid from a biological sample having the formula ##STR3## wherein the quaternary amine is an alkyltrimethylammonium, wherein R1, R2, and R3 are alkyl groups of one carbon and R4 is an alkyl selected from the group consisting of 12, 14, or 16 carbons in length, and X - is selected from the group consisting of oxalate, malonate and succinate.
Claim 5
Dependent Claim
67 words
A quaternary amine surfactant useful for isolating nucleic acid from a biological sample having the formula ##STR3## wherein the quaternary amine is an alkyltrimethylammonium, wherein R1, R2, and R3 are alkyl groups of one carbon and R4 is an alkyl selected from the group consisting of 12, 14, or 16 carbons in length, and X - is selected from the group consisting of oxalate, malonate and succinate.
Claim 6
Dependent Claim
21 words
The surfactant according to claim 3 wherein R4 is an alkyl group 14 carbons in length, and X - is oxalate.
Claim 7
Dependent Claim
101 words
A kit comprising an aqueous quaternary amine surfactant, wherein said quaternary amine is useful for isolating ribonucleic acid from a biological sample and has the formula ##STR4## wherein R1 through R4 are independently selected from an alkyl chain containing from 1 to 20 carbons, and an aryl group containing from 6 to 26 carbons and X - is selected from the group consisting of propionate, oxalate, malonate, succinate and citrate; and reagents for separating and dissociating the RNA from the biological sample, said reagents selected from the group consisting of formamide solvent, guanidinium isothiocyanate solution, lithium chloride solution and ethanolic solution.
Claim 8
Dependent Claim
101 words
A kit comprising an aqueous quaternary amine surfactant, wherein said quaternary amine is useful for isolating ribonucleic acid from a biological sample and has the formula ##STR4## wherein R1 through R4 are independently selected from an alkyl chain containing from 1 to 20 carbons, and an aryl group containing from 6 to 26 carbons and X - is selected from the group consisting of propionate, oxalate, malonate, succinate and citrate; and reagents for separating and dissociating the RNA from the biological sample, said reagents selected from the group consisting of formamide solvent, guanidinium isothiocyanate solution, lithium chloride solution and ethanolic solution.
Claim 9
Dependent Claim
56 words
The kit according to claim 5 wherein the quaternary amine is an alkyltrimethylammonium, wherein R1, R2 and R3 are alkyl groups of one carbon and R4 is an alkyl selected from the group consisting of 12, 14, or 16 carbons in length, and X - is selected from the group consisting of oxalate, malonate, and succinate.
Claim 10
Dependent Claim
21 words
The kit according to claim 6 wherein R4 is an alkyl group 14 carbons in length, and X - is oxalate.
Claim 11
Dependent Claim
58 words
The kit according to claim 5 wherein the quaternary amine is on alkylbenzyldimethylammonium, wherein R1 and R2 are alkyl groups of 1 carbon in length, R3 is an aryl group of 6 carbons, and R4 is an alkyl selected from the group consisting of 12, 14, 16, or 18 carbons in length, and the X - is propionate.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2001-03-16 | 2001-09-15 | 2002-03-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2005-03-16 | 2005-09-15 | 2006-03-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2009-03-16 | 2009-09-15 | 2010-03-17 | $7700.00 | Window Closed / Lapsed |