US5733727A
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Myocardial grafts and cellular compositions
Assignee: Indiana University Research and Technology Corp
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Filing: 1995-06-07
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Granted: 1998-03-31
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Expires: 2015-06-07
Abstract
Abstract Described are preferred myocardial grafts of skeletal myoblasts or cardiomyocytes, and cellular compositions and methods useful in obtaining the grafts. The myocardial grafts are stable and can be used, for example, to deliver recombinant proteins directly to the heart.
Inventors
- 👤 Loren J. Field
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 04:49
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/06; C12N5/0602; C12N5/0652; C12N5/0658; A; A01; A01K; A01K67/00; A01K67/027; A01K67/0271; A61; A61K; A61K35/00; A61K35/12; A61K35/34; A61K48/00; C07; C07K; C07K14/00; C07K14/435; C07K14/46; C07K14/47; C07K14/4701; C07K14/4716; C12N5/0657; A01K2217/00; A01K2217/05; C12N2500/00; C12N2500/05; C12N2500/10; C12N2500/20; C12N2500/22; C12N2506/00; C12N2506/02; C12N2510/00; C12N2517/00; C12N2517/02
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
56 words
A method of obtaining a population of cells, comprising: transfecting embryonic stem cells to introduce a marker gene enabling selection of a cardiomyocyte cell lineage from other cell lineages resulting from the differentiation of the stem cells; causing the stem cells to differentiate in culture; and selecting said cardiomyocyte cell lineage based on said marker gene.
Claim 2
Independent Claim
56 words
A method of obtaining a population of cells, comprising: transfecting embryonic stem cells to introduce a marker gene enabling selection of a cardiomyocyte cell lineage from other cell lineages resulting from the differentiation of the stem cells; causing the stem cells to differentiate in culture; and selecting said cardiomyocyte cell lineage based on said marker gene.
Claim 3
Dependent Claim
73 words
The method of claim 1, comprising: transfecting the stem cells to introduce (i) a first marker gene enabling selection of transfected stem cells from non-transfected stem cells and (ii) a second marker gene enabling selection of said one cell lineage from said other cell lineages; selecting transfected stern cells based on the first marker gene; causing the selected stem cells to differentiate; and selecting said one cell lineage based on said marker gene.
Claim 4
Dependent Claim
30 words
An embryonic stem cell having introduced DNA encoding a marker gene enabling selection of a cardiomyocyte cell lineage from other cell lineages resulting from the differentiation of the stem cells.
Claim 5
Dependent Claim
30 words
An embryonic stem cell having introduced DNA encoding a marker gene enabling selection of a cardiomyocyte cell lineage from other cell lineages resulting from the differentiation of the stem cells.
Claim 6
Dependent Claim
55 words
The stem cell of claim 3 which includes introduced DNA encoding (i) a first marker gene enabling selection of embryonic stem cells having the first marker gene from embryonic stem cells not having the first marker gene and (ii) a second marker gene enabling selection of said cardiomyocyte cell lineage from said other cell lineages.
Claim 7
Dependent Claim
20 words
The stem cell of claim 3, wherein said marker gene confers antibiotic resistance to cells which express the marker gene.
Claim 8
Dependent Claim
24 words
The stem cell of claim 4, wherein said first and second marker genes each confer antibiotic resistance to cells which express the marker genes.
Claim 9
Dependent Claim
31 words
The stem cell of claim 6, wherein said second marker gene is under the control of a promoter which causes expression of the marker gene only in said cardiomyocyte cell lineage.
Claim 10
Independent Claim
86 words
A method of obtaining a population of cells, comprising: providing an embryonic stem cell having introduced DNA encoding a marker gene enabling selection of a cardiomyocyte cell lineage from other cell lineages resulting from the differentiation of the stem cells; causing the stem cell to differentiate in culture to form a mixed differentiated cellular population containing said a cardiomyocyte cell lineage and said other cell lineages; selecting from the cellular population said a cardiomyocyte cell lineage from said other cell lineages based on said marker gene.
Claim 11
Independent Claim
86 words
A method of obtaining a population of cells, comprising: providing an embryonic stem cell having introduced DNA encoding a marker gene enabling selection of a cardiomyocyte cell lineage from other cell lineages resulting from the differentiation of the stem cells; causing the stem cell to differentiate in culture to form a mixed differentiated cellular population containing said a cardiomyocyte cell lineage and said other cell lineages; selecting from the cellular population said a cardiomyocyte cell lineage from said other cell lineages based on said marker gene.
Claim 12
Dependent Claim
51 words
The method of claim 8 in which: said marker gene is under the control of a promoter which causes expression of the marker gene in said cardiomyocyte cell lineage but not in said other cell lineages, so as to enable selection of said cardiomyocyte cell lineage from said other cell lineages.
Claim 13
Dependent Claim
68 words
The method of claim 9, in which said marker gene confers antibiotic resistance, and wherein said selecting includes contacting the mixed differentiated cellular population with an antibiotic to which cells of said one cell lineage have been conferred resistance by the expression of said first marker gene, thereby killing cells of said other cell lineages but not cells of said one cell lineage which express the marker gene.
Claim 14
Dependent Claim
16 words
The method of claim 9, in which said marker gene confers resistance to neomycin or hygromycin.
Claim 15
Dependent Claim
13 words
A population of cardiomyocyte cells obtained by a method according to claim 8.
Claim 16
Dependent Claim
17 words
The population of cells of claim 12, which is obtained by a method according to claim 10.
References Cited (Backward Citations)
No citation records indexed.
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| 3.5 Years (Year 4) | 2001-03-30 | 2001-09-29 | 2002-03-31 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2005-03-30 | 2005-09-29 | 2006-03-31 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2009-03-30 | 2009-09-29 | 2010-03-31 | $7700.00 | Window Closed / Lapsed |