US5789160A
Expired / Lapsed
US
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Assignee: MEDALLION BIOMEDICAL LLC
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Filing: 1995-06-05
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Granted: 1998-08-04
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Expires: 2015-06-05
Abstract
Abstract This invention disclosures a method for coevolving products from two or more reactants, along with the nucleic acid that can facilitate the reaction for making the products. The invention further discloses the products and facilitating nucleic acids produced by said method.
Inventors
- 👤 Bruce E. Eaton
- 👤 Larry Gold
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 08:54
CPC Classifications
C; C07; C07H; C07H21/00; A; A61; A61K; A61K47/00; A61K47/50; A61K47/51; A61K47/54; A61K47/547; A61K47/549; B; B82; B82Y; B82Y5/00; C07H19/00; C07H19/02; C07H19/04; C07H19/06; C07H19/10; C07K; C07K14/00; C07K14/001; C12; C12N; C12N15/00; C12N15/09; C12N15/10; C12N15/1034; C12N15/1048; C12N15/11; C12N15/115; C12N9/00; C12N9/10; C12N9/12; C12N9/1241; C12N9/1276; C12Q; C12Q1/00; C12Q1/34; C12Q1/37; C12Q1/68; C12Q1/6811; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; G01N33/535; G01N33/569; G01N33/56983; G01N33/56988; G01N33/68; G01N33/6803; G01N33/6842; G01N33/74; G01N33/76; C12N2310/00; C12N2310/10; C12N2310/13; C12N2310/30; C12N2310/32; C12N2310/322; C12N2310/50; C12N2310/53; C40; C40B; C40B40/00; F; F02; F02B; F02B75/00; F02B75/02; F02B2075/022; F02B2075/027; G01N2333/00; G01N2333/005; G01N2333/08; G01N2333/15; G01N2333/155; G01N2333/16; G01N2333/163; G01N2333/435; G01N2333/475; G01N2333/50; G01N2333/503; G01N2333/575; G01N2333/62; G01N2333/81; G01N2333/8107; G01N2333/811; G01N2333/8121; G01N2333/8125; G01N2333/90; G01N2333/914; G01N2333/948; G01N2333/95; G01N2333/964; G01N2333/96425; G01N2333/96427; G01N2333/9643; G01N2333/96433; G01N2333/96436; G01N2333/96441; G01N2333/96455; G01N2333/96486; G01N2333/966; G01N2333/972; G01N2333/9726; G01N2333/974; G01N2333/976
Claims Analysis
Total Claims: 24
Claim 1
Independent Claim
154 words
A method for identifying a product from a product library, wherein said product binds to a target, said method comprising: (a) preparing a nucleic acid-first reactant test mixture comprised of nucleic acids each having a region of randomized sequence and each being coupled to a first reactant consisting of a small organic molecule with a molecular weight in the range of 2 to 1000; (b) reacting said nucleic acid-first reactant test mixture with a free reactant consisting of a small organic molecule with a molecular weight in the range of 2 to 1000 to form a product library comprised of products formed by the reaction of said first reactant and said free reactant, wherein said reaction is facilitated by the nucleic acid coupled to said first reactant; (c) partitioning between members of said product library based on their ability to bind to said target; and (d) identifying said products which bind to said target.
Claim 2
Independent Claim
154 words
A method for identifying a product from a product library, wherein said product binds to a target, said method comprising: (a) preparing a nucleic acid-first reactant test mixture comprised of nucleic acids each having a region of randomized sequence and each being coupled to a first reactant consisting of a small organic molecule with a molecular weight in the range of 2 to 1000; (b) reacting said nucleic acid-first reactant test mixture with a free reactant consisting of a small organic molecule with a molecular weight in the range of 2 to 1000 to form a product library comprised of products formed by the reaction of said first reactant and said free reactant, wherein said reaction is facilitated by the nucleic acid coupled to said first reactant; (c) partitioning between members of said product library based on their ability to bind to said target; and (d) identifying said products which bind to said target.
Claim 3
Dependent Claim
27 words
The method of claim 1 wherein said nucleic acid-first reactant test mixture comprises nucleic acids having a region of conserved sequences and a region of randomized sequences.
Claim 4
Dependent Claim
22 words
The method of claim 1 wherein said nucleic acid is selected from the group of single-stranded RNA, single-stranded DNA and double-stranded DNA.
Claim 5
Dependent Claim
16 words
The method of claim 1 wherein said nucleic acid first reactant test mixture comprises modified nucleotides.
Claim 6
Dependent Claim
23 words
The method of claim 1 further comprising an organometallic catalyst covalently linked to said nucleic acids of said nucleic acid-first reactant test mixture.
Claim 7
Dependent Claim
20 words
The method of claim 1 which further comprises a linker group coupled between said first reactant and said nucleic acid.
Claim 8
Dependent Claim
25 words
The method of claim 1 wherein said first reactant is a dienophile, said free reactant is a diene, and said product is a cyclohexene derivative.
Claim 9
Dependent Claim
27 words
The method of claim 1 wherein said nucleic acid-first reactant test mixture comprises an organometallic catalyst in solution with said nucleic acids coupled to said first reactant.
Claim 10
Dependent Claim
21 words
The method of claim 4 wherein said modified nucleotides have been chemically modified at the ribose and/or phosphate and/or base positions.
Claim 11
Dependent Claim
18 words
The method of claim 4 wherein said modified mucleotides are pyrimidines modified at the 2'- or 5- positions.
Claim 12
Dependent Claim
16 words
The method of claim 6 wherein said modified nucleotides are purines modified at the 8- position.
Claim 13
Dependent Claim
28 words
The method of claim 6 wherein said modified nucleotides are modified with a chemical group which increases charge, polarizability, hydrogen bonding, electrostatic interaction or fluxionality of the nucleotide.
Claim 14
Dependent Claim
20 words
The method of claim 6 wherein said linker group has a size in the range of 10 to 1000 Å.
Claim 15
Dependent Claim
40 words
The method of claim 12 wherein said chemical group is selected from the group consisting of hydrophobic moieties, hydrophilic moieties, metal atoms in various oxidation states, rigid structures, imidazoles, primary alcohols, carboxylates, guanidium groups, amino groups, thiols and organometallic catalysts.
Claim 16
Dependent Claim
18 words
The method of claim 12 wherein said chemical group comprises an amino acid side chain or analogs thereof.
Claim 17
Dependent Claim
23 words
The method of claim 13 wherein said linker group is selected from the group consisting of polyethylene glycol, polyvinyl alcohol, polyacrylates and polypeptides.
Claim 18
Independent Claim
144 words
A method for identifying a product from a product library, wherein said product binds to a target molecule, said method comprising: (a) preparing a nucleic acid-first reactant test mixture comprises of nucleic acids each having a region of randomized sequence and each being coupled to a first reactant consisting of a small organic molecule with a molecular weight in the range of 2 to 1000; (b) reacting said nucleic acid-first reactant test mixture with a free reactant consisting of a small organic molecule with a molecular weight in the range of 2 to 1000 to form a product library comprised of nucleic acids coupled to a product formed by the reaction of said first reactant and said free reactant; (c) partitioning said products having increased affinity to the target from the remainder of the product library; and (d) identifying said product from said library.
Claim 19
Independent Claim
144 words
A method for identifying a product from a product library, wherein said product binds to a target molecule, said method comprising: (a) preparing a nucleic acid-first reactant test mixture comprises of nucleic acids each having a region of randomized sequence and each being coupled to a first reactant consisting of a small organic molecule with a molecular weight in the range of 2 to 1000; (b) reacting said nucleic acid-first reactant test mixture with a free reactant consisting of a small organic molecule with a molecular weight in the range of 2 to 1000 to form a product library comprised of nucleic acids coupled to a product formed by the reaction of said first reactant and said free reactant; (c) partitioning said products having increased affinity to the target from the remainder of the product library; and (d) identifying said product from said library.
Claim 20
Dependent Claim
29 words
The method of claim 17 which further comprises between steps b and c, contacting the product library with a non-target and partitioning away products which bind to said non-target.
Claim 21
Independent Claim
150 words
A method for identifying a facilitating nucleic acid, wherein said nucleic acid is selected for its ability to facilitate a reaction between a first reactant coupled to said nucleic acid and a free reactant, wherein each reactant is consisting of a small organic molecule with a molecular weight in the range of 2 to 1000, to form a product which binds to a target, said method comprising: (a) preparing a nucleic acid-first reactant test mixture comprised of nucleic acids each having a region of randomized sequence and each being coupled to a first reactant; (b) mixing said nucleic acid-first reactant test mixture with a plurality of free reactants under conditions favorable for a nucleic acid facilitated reaction between said first reactant and said free reactant to form said product; (c) isolating members of said nucleic acid-first reactant test mixture which formed said products; and (d) identifying said facilitating nucleic acid.
Claim 22
Independent Claim
150 words
A method for identifying a facilitating nucleic acid, wherein said nucleic acid is selected for its ability to facilitate a reaction between a first reactant coupled to said nucleic acid and a free reactant, wherein each reactant is consisting of a small organic molecule with a molecular weight in the range of 2 to 1000, to form a product which binds to a target, said method comprising: (a) preparing a nucleic acid-first reactant test mixture comprised of nucleic acids each having a region of randomized sequence and each being coupled to a first reactant; (b) mixing said nucleic acid-first reactant test mixture with a plurality of free reactants under conditions favorable for a nucleic acid facilitated reaction between said first reactant and said free reactant to form said product; (c) isolating members of said nucleic acid-first reactant test mixture which formed said products; and (d) identifying said facilitating nucleic acid.
Claim 23
Independent Claim
111 words
A method for simultaneously identifying facilitating nucleic acids and producing a product library, wherein said facilitating nucleic acids facilitate the reaction between a first reactant and at least one free reactant, wherein each reactant is consisting of a small organic molecule with a molecular weight in the range of 2 to 1000, to form said product library, said method comprising: (a) preparing a nucleic acid-first reactant test mixture comprised of nucleic acids each having a region of randomized sequence and each being coupled to said first reactant; (b) reacting said nucleic acid-first reactant test mixture with said free reactant to form a product library; and (c) identifying said facilitating nucleic acids.
Claim 24
Independent Claim
111 words
A method for simultaneously identifying facilitating nucleic acids and producing a product library, wherein said facilitating nucleic acids facilitate the reaction between a first reactant and at least one free reactant, wherein each reactant is consisting of a small organic molecule with a molecular weight in the range of 2 to 1000, to form said product library, said method comprising: (a) preparing a nucleic acid-first reactant test mixture comprised of nucleic acids each having a region of randomized sequence and each being coupled to said first reactant; (b) reacting said nucleic acid-first reactant test mixture with said free reactant to form a product library; and (c) identifying said facilitating nucleic acids.
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