US5804047A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Enzyme-immobilized electrode, composition for preparation of the same and electrically conductive enzyme
Assignee: Dai Nippon Printing Co Ltd
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Filing: 1994-12-16
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Granted: 1998-09-08
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Expires: 2014-12-16
Abstract
Abstract An enzyme sensor system capable of quantitatively determining a particular component contained in a biological sample in a rapid and easy manner is disclosed. More particularly, an electrically conductive enzyme, an enzyme-immobilized electrode using the same and a composition for use in the preparation of the enzyme electrode are disclosed. The composition for an enzyme-immobilized electrode according to the present invention enables electrodes of various patterns to be produced by screen printing.
Inventors
- 👤 Isao Karube
- 👤 Susan Anne Clark
- 👤 Ryohei Nagata
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 18:48
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/001; C12Q1/005; C12Q1/002; C12Q1/003; C12Q1/004; Y; Y10; Y10S; Y10S435/00; Y10S435/817
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
109 words
A process for producing an electrically conductive enzyme comprising an enzyme and a mediator attached to a side chain and the body of the enzyme through a covalent bond wherein said enzyme is glucose oxidase and said mediator is a ferrocene derivative attached to the side chain of the enzyme through a spacer, comprising the steps of oxidizing a glucose oxidase with an oxidizing agent to form an aldehyde group on an oligosaccharide of the glucose oxidase; reacting said glucose oxidase with a dihydrazide represented by the following formula (II): ##STR5## wherein n is an integer of 1 to 7; and reacting the reaction product with a ferrocenecarboxylic acid.
Claim 2
Dependent Claim
109 words
A process for producing an electrically conductive enzyme comprising an enzyme and a mediator attached to a side chain and the body of the enzyme through a covalent bond wherein said enzyme is glucose oxidase and said mediator is a ferrocene derivative attached to the side chain of the enzyme through a spacer, comprising the steps of oxidizing a glucose oxidase with an oxidizing agent to form an aldehyde group on an oligosaccharide of the glucose oxidase; reacting said glucose oxidase with a dihydrazide represented by the following formula (II): ##STR5## wherein n is an integer of 1 to 7; and reacting the reaction product with a ferrocenecarboxylic acid.
Claim 3
Dependent Claim
28 words
An enzyme-immobilized electrode comprising a base material and an electrically conductive enzyme obtained by the process according to claim 1, the enzyme being immobilized on the base material.
Claim 4
Dependent Claim
27 words
A composition for preparing an enzyme-immobilized electrode, comprising an electrically conductive enzyme obtained by the process according to claim 1, an electrically conductive component and a vehicle.
Claim 5
Dependent Claim
20 words
A process for preparing an enzyme-immobilized electrode which comprises immobilizing the composition according to claim 3 on a base material.
Claim 6
Dependent Claim
34 words
A process according to claim 4, wherein the composition is immobilized on the base material by a printing technique selected from the group consisting of a screen printing and roller coating and with dispenser.
Claim 7
Dependent Claim
29 words
The process for producing an electrically conductive enzyme according to claim 1, wherein said reacting is carried out in the presence of a reducing agent or a condensing agent.
Claim 8
Dependent Claim
30 words
A process for preparing an enzyme-immobilized electrode which comprises immobilizing a composition on a base material, the composition comprising an electrically conductive enzyme, an electrically conductive component and a vehicle.
Claim 9
Dependent Claim
30 words
A process for preparing an enzyme-immobilized electrode which comprises immobilizing a composition on a base material, the composition comprising an electrically conductive enzyme, an electrically conductive component and a vehicle.
References Cited (Backward Citations)
DE277759C
Cited
EP0126043A1
Cited
WO1985005638A1
Cited
EP0278647A2
Cited
EP0294294A2
Cited
JPH01117781A
Cited
US5089112A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2001-09-07 | 2002-03-09 | 2002-09-08 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2005-09-07 | 2006-03-09 | 2006-09-08 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2009-09-07 | 2010-03-09 | 2010-09-08 | $7700.00 | Window Closed / Lapsed |