US5858660A
Expired / Lapsed
US
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Assignee: MEDALLION BIOMEDICAL LLC
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Filing: 1996-03-20
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Granted: 1999-01-12
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Expires: 2016-03-20
Abstract
Abstract This invention discloses a method for coevolving products from two or more reactants, along with the nucleic acid that can facilitate the reaction for making the products. The invention further discloses the products and facilitating nucleic acids produced by said method.
Inventors
- 👤 Bruce Eaton
- 👤 Larry Gold
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 14:09
CPC Classifications
C; C07; C07H; C07H21/00; A; A61; A61K; A61K47/00; A61K47/50; A61K47/51; A61K47/54; A61K47/547; A61K47/549; B; B82; B82Y; B82Y5/00; C07H19/00; C07H19/02; C07H19/04; C07H19/06; C07H19/10; C07K; C07K14/00; C07K14/001; C12; C12N; C12N15/00; C12N15/09; C12N15/10; C12N15/1034; C12N15/1048; C12N15/11; C12N15/115; C12N9/00; C12N9/10; C12N9/12; C12N9/1241; C12N9/1276; C12Q; C12Q1/00; C12Q1/34; C12Q1/37; C12Q1/68; C12Q1/6811; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; G01N33/535; G01N33/569; G01N33/56983; G01N33/56988; G01N33/68; G01N33/6803; G01N33/6842; G01N33/74; G01N33/76; C12N2310/00; C12N2310/10; C12N2310/13; C12N2310/30; C12N2310/32; C12N2310/322; C12N2310/50; C12N2310/53; C40; C40B; C40B40/00; F; F02; F02B; F02B75/00; F02B75/02; F02B2075/022; F02B2075/027; G01N2333/00; G01N2333/005; G01N2333/08; G01N2333/15; G01N2333/155; G01N2333/16; G01N2333/163; G01N2333/435; G01N2333/475; G01N2333/50; G01N2333/503; G01N2333/575; G01N2333/62; G01N2333/81; G01N2333/8107; G01N2333/811; G01N2333/8121; G01N2333/8125; G01N2333/90; G01N2333/914; G01N2333/948; G01N2333/95; G01N2333/964; G01N2333/96425; G01N2333/96427; G01N2333/9643; G01N2333/96433; G01N2333/96436; G01N2333/96441; G01N2333/96455; G01N2333/96486; G01N2333/966; G01N2333/972; G01N2333/9726; G01N2333/974; G01N2333/976
Claims Analysis
Total Claims: 23
Claim 1
Independent Claim
210 words
A method for producing a product having the ability to perform a preselected function on a target comprising: (a) preparing a nucleic acid test mixture; (b) coupling each member of said nucleic acid test mixture with a first reactant consisting of a small organic molecule with a molecular weight in the range of 2 to 1000 to form a nucleic acid-first reactant test mixture; (c) forming a product library by contacting said nucleic acid-first reactant test mixture with a mixture of free reactants consisting of small organic molecules with a molecular weight in the range of 2 to 1000, wherein said product library is formed as a result of a bond formation reaction between said first reactant and at least one of said free reactants, wherein said bond formation reaction is facilitated by a nucleic acid coupled to said first reactant; (d) contacting the product library of step (c) with a target, wherein the product having the ability to perform a preselected function on said target relative to the product library may be partitioned from the remainder of the product library; and (e) partitioning said product having said ability to perform a preselected function on said target from the remainder of the product library, whereby said product can be identified.
Claim 2
Independent Claim
210 words
A method for producing a product having the ability to perform a preselected function on a target comprising: (a) preparing a nucleic acid test mixture; (b) coupling each member of said nucleic acid test mixture with a first reactant consisting of a small organic molecule with a molecular weight in the range of 2 to 1000 to form a nucleic acid-first reactant test mixture; (c) forming a product library by contacting said nucleic acid-first reactant test mixture with a mixture of free reactants consisting of small organic molecules with a molecular weight in the range of 2 to 1000, wherein said product library is formed as a result of a bond formation reaction between said first reactant and at least one of said free reactants, wherein said bond formation reaction is facilitated by a nucleic acid coupled to said first reactant; (d) contacting the product library of step (c) with a target, wherein the product having the ability to perform a preselected function on said target relative to the product library may be partitioned from the remainder of the product library; and (e) partitioning said product having said ability to perform a preselected function on said target from the remainder of the product library, whereby said product can be identified.
Claim 3
Independent Claim
244 words
A method for coproducing a nucleic acid that facilitates bond formation and a product that performs a preselected function on a target comprising: (a) preparing a nucleic acid test mixture; (b) coupling a first reactant consisting of a small organic molecule with a molecular weight in the range of 2 to 1000 to each member of said nucleic acid test mixture to form a nucleic acid-first reactant test mixture; (c) forming a product library by contacting said nucleic acid-first reactant test mixture with a mixture of free reactants consisting of small organic molecules with a molecular weight in the range of 2 to 1000, wherein said product library is formed as a result of a bond formation reaction between said first reactant and at least one of said free reactants, wherein said bond formation is facilitated by a nucleic acid which is coupled to said first reactant and wherein each product is coupled to a nucleic acid; (d) contacting said product library with a target, wherein products that perform a preselected function on said target relative to the product library may be partitioned from the remainder of the product library; and (e) amplifying the nucleic acid associated with the product that performs a preselected function on said target, to yield a mixture of nucleic acids enriched for nucleic acids that facilitate bond formation between said first reactant and said free reactant, whereby said nucleic acid that facilitates bond formation and said product are produced.
Claim 4
Independent Claim
244 words
A method for coproducing a nucleic acid that facilitates bond formation and a product that performs a preselected function on a target comprising: (a) preparing a nucleic acid test mixture; (b) coupling a first reactant consisting of a small organic molecule with a molecular weight in the range of 2 to 1000 to each member of said nucleic acid test mixture to form a nucleic acid-first reactant test mixture; (c) forming a product library by contacting said nucleic acid-first reactant test mixture with a mixture of free reactants consisting of small organic molecules with a molecular weight in the range of 2 to 1000, wherein said product library is formed as a result of a bond formation reaction between said first reactant and at least one of said free reactants, wherein said bond formation is facilitated by a nucleic acid which is coupled to said first reactant and wherein each product is coupled to a nucleic acid; (d) contacting said product library with a target, wherein products that perform a preselected function on said target relative to the product library may be partitioned from the remainder of the product library; and (e) amplifying the nucleic acid associated with the product that performs a preselected function on said target, to yield a mixture of nucleic acids enriched for nucleic acids that facilitate bond formation between said first reactant and said free reactant, whereby said nucleic acid that facilitates bond formation and said product are produced.
Claim 5
Dependent Claim
47 words
The method of claim 2 which further comprises: (f) coupling the amplified nucleic acids with said first reactant; and (g) repeating steps (c) through (f) until the product having said ability to perform said preselected function on said target is produced in sufficient quantity for structure determination.
Claim 6
Independent Claim
92 words
A method for producing a product library comprising contacting a mixture of first reactants each coupled to a member of a nucleic acid test mixture of free reactants, each first and free reactant consisting of a small organic molecule with a molecular weight in the range of 2 to 1000, wherein said product library is formed as a result of a bond formation reaction between said first reactant and at least one of said free reactants, wherein said bond formation reaction is facilitated by the nucleic acid coupled to said first reactant.
Claim 7
Independent Claim
92 words
A method for producing a product library comprising contacting a mixture of first reactants each coupled to a member of a nucleic acid test mixture of free reactants, each first and free reactant consisting of a small organic molecule with a molecular weight in the range of 2 to 1000, wherein said product library is formed as a result of a bond formation reaction between said first reactant and at least one of said free reactants, wherein said bond formation reaction is facilitated by the nucleic acid coupled to said first reactant.
Claim 8
Dependent Claim
26 words
The method of claim 1 wherein said nucleic acid test mixture comprises nucleic acids having a region of conserved sequences and a region of randomized sequences.
Claim 9
Dependent Claim
28 words
The method of claim 1 wherein said nucleic acid coupled to said first reactant is selected from the group consisting of single-stranded RNA, single-stranded DNA and double-stranded DNA.
Claim 10
Dependent Claim
19 words
The method of claim 1 wherein said nucleic acid test mixture comprises pyrimidines modified at the 2'- or 5-positions.
Claim 11
Dependent Claim
17 words
The method of claim 1 wherein said nucleic acid test mixture comprises purines modified at the 8-position.
Claim 12
Dependent Claim
20 words
The method of claim 1 which further comprises a linker group coupled between said first reactant and said nucleic acid.
Claim 13
Dependent Claim
19 words
The method of claim 9 wherein said linker group has a size in the range of 10 to 1000.
Claim 14
Dependent Claim
22 words
The method of claim 10 wherein said linker group is selected from the group consisting of PEG, polyvinyl alcohol, polyacrylates and polypeptides.
Claim 15
Dependent Claim
24 words
The method of claim 1 wherein said first reactant is a dienophile, said free reactant is a diene, and said product is a cyclohexene.
Claim 16
Dependent Claim
26 words
The method of claim 2 wherein said nucleic acid test mixture comprises nucleic acids having a region of conserved sequences and a region of randomized sequences.
Claim 17
Dependent Claim
28 words
The method of claim 2 wherein said nucleic acid coupled to said first reactant is selected from the group consisting of single-stranded RNA, single-stranded DNA and double-stranded DNA.
Claim 18
Dependent Claim
19 words
The method of claim 2 wherein said nucleic acid test mixture comprises pyrimidines at the 2'- or 5- positions.
Claim 19
Dependent Claim
17 words
The method of claim 2 wherein said nucleic acid test mixture comprises purines modified at the 8-position.
Claim 20
Dependent Claim
20 words
The method of claim 2 which further comprises a linker group coupled between said first reactant and said nucleic acid.
Claim 21
Dependent Claim
19 words
The method of claim 17 wherein said linker group has a size in the range of 10 to 1000.
Claim 22
Dependent Claim
22 words
The method of claim 18 wherein said linker group is selected from the group consisting of PEG, polyvinyl alcohol, polyacrylates and polypeptides.
Claim 23
Dependent Claim
24 words
The method of claim 2 wherein said first reactant is a dienophile, said free reactant is a diene, and said product is a cyclohexene.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 2002-01-11 | 2002-07-13 | 2003-01-12 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2006-01-11 | 2006-07-13 | 2007-01-12 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2010-01-11 | 2010-07-13 | 2011-01-12 | $7700.00 | Window Closed / Lapsed |