US6008038A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method and a circuit arrangement for the electropermeation of living cells
Assignee: Eppendorf SE
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Filing: 1998-03-18
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Granted: 1999-12-28
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Expires: 2018-03-18
Abstract
Abstract A method for the electropermeation of living cells between at least two electrodes over a predetermined interval with an electrical potential or a current, characterised by the following features: a voltage or current source which is not exhaustable over the interval is applied to the electrodes via a controllable power semiconductor functioning as a regulator; the course of the potential or of the current during the interval is predetermined by a setting value transducer which generates any selectable course of curve, and is inputted to a controller which is connected in front of the driver step for the power semiconductor; the controller receives a value, as an instantaneous value, corresponding to the actual potential or current applied at the electrodes; the interval has a time range of a few 100 ns to several minutes.
Inventors
- 👤 Wolfgang Kroger
- 👤 Bernd Jagdhuber
- 👤 Hans-Joachim Ricklefs
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 13:59
CPC Classifications
C; C12; C12M; C12M35/00; C12M35/02
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
138 words
A circuit arrangement for the electropermeation of living cells with a voltage or current source, which during a predetermined interval can be connected via a controllable semiconductor to electrodes and with a device for triggering the controllable semiconductor, wherein the voltage or current source is connected, via the controllable semiconductor (14) functioning as regulating element, to the electrodes (10), the regulating signals for the driver step (16) of the controllable semiconductor (14) are produced by an electronic control circuit, to which there is connected a setting value transducer which predetermines the course of the potential or of the current, as well as an instantaneous value transducer acquiring the potential or the current at the electrodes (10), and the output of the electronic control circuit is coupled to the driver stage (16) via potential separating stage (22, 30; 26).
Claim 2
Dependent Claim
138 words
A circuit arrangement for the electropermeation of living cells with a voltage or current source, which during a predetermined interval can be connected via a controllable semiconductor to electrodes and with a device for triggering the controllable semiconductor, wherein the voltage or current source is connected, via the controllable semiconductor (14) functioning as regulating element, to the electrodes (10), the regulating signals for the driver step (16) of the controllable semiconductor (14) are produced by an electronic control circuit, to which there is connected a setting value transducer which predetermines the course of the potential or of the current, as well as an instantaneous value transducer acquiring the potential or the current at the electrodes (10), and the output of the electronic control circuit is coupled to the driver stage (16) via potential separating stage (22, 30; 26).
Claim 3
Dependent Claim
15 words
A circuit arrangement according to claim 1, wherein the controllable semiconductor (14) is an IGBT.
Claim 4
Dependent Claim
33 words
A circuit arrangement according to claim 1 or 2, wherein the setting value transducer comprises a computer (40) whose step-shaped output signal is inputted to the control circuit via a digital-analog converter (38).
Claim 5
Dependent Claim
58 words
A circuit arrangement according to claim 1, wherein the coupling of the control circuit to the driver stage (16) is effected in parallel via an optocoupler (30, 22), and a transformer (26), and the circuit arrangement further comprises a summing stage (20) in which output signals from the optocoupler and the transformer are summed in a summing step.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2002-12-27 | 2003-06-28 | 2003-12-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2006-12-27 | 2007-06-28 | 2007-12-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2010-12-27 | 2011-06-28 | 2011-12-28 | $7700.00 | Window Closed / Lapsed |