Enzyme electrode structure

Claim 2

A biosensor comprising an upper plate, a lower plate, a space formed between the two plates for sucking and housing a sample therein, a layer for fixing the two plates together, the space for sucking and housing the sample being partially opened at a peripheral part thereof and partially closed by the layer, a working electrode provided on at least one of the two plates and having at least glucose oxidase and a counter electrode fixed relative to the working electrode, each of the two plates lacking an air vent or hole for connecting to the space, one end of each of the two plates having a tapered part, and a tip part of the working electrode or a tip part of the counter electrode provided on the tapered part.

Claim 3

A biosensor according to claim 1, wherein the working electrode and the counter electrode are formed on the upper and lower plates, respectively, so as to face each other.

Claim 4

A biosensor according to claim 1, wherein an oxidoreductase-electron transmitter mixture layer is provided on the working electrode.

Claim 5

A biosensor according to claim 1, wherein the glucose oxidase is fixed by applying and drying.

Claim 6

A biosensor according to claim 1, wherein the layer is an adhesive layer formed by a double-sided adhesive tape.

Claim 7

A biosensor according to claim 1, wherein the layer is an adhesive layer or a spacer with adhesive on both sides thereof which is interposed between the two plates.

Claim 8

A biosensor according to claim 1, wherein a reference electrode is arranged at a side of the working electrode or the counter electrode.

Claim 9

A biosensor according to claim 1, wherein the layer is an adhesive layer formed by applying an adhesive to one of the two plates.

Claim 10

A biosensor according to claim 1, wherein the layer is an adhesive layer formed by applying an adhesive to both the plates.

Claim 11

A biosensor according to claim 1, wherein an insulating layer is formed between one of the two plates and the layer.

Claim 12

A biosensor according to claim 1, wherein one end of each of the plates is formed into a protruding shape.

Claim 13

A biosensor according to claim 1, wherein an oxidoreductase is fixed on the counter electrode.

Claim 14

A biosensor according to claim 1, wherein the space is closed by a closing edge of the layer which extends between two opposite side edges of the two plates, and the space is open at a peripheral portion of the two plates outside of the edge of the layer where the layer is not provided.

Claim 15

A biosensor according to claim 13, wherein the closing edge of the layer is substantially straight.

Claim 16

A biosensor which comprises a lower base, an upper base, a working electrode, an oxidoreductase fixed on the working electrode, and a counter electrode, wherein the working electrode and the counter electrode are fixed on the lower base and the upper base through an adhesive layer so as to have a facing structure, and a lead part for each of the two electrodes is formed in a position of the lower base where an end part thereof is never superposed on the upper base, the electrode on the upper base being conducted to its lead part through the adhesive layer.

Claim 17

A biosensor which comprises a lower base, an upper base, a working electrode, an oxidoreductase fixed on the working electrode, and a counter electrode, wherein the working electrode and the counter electrode are fixed on the lower base and the upper base through an adhesive layer so as to have a facing structure, and a lead part for each of the two electrodes is formed in a position of the lower base where an end part thereof is never superposed on the upper base, the electrode on the upper base being conducted to its lead part through the adhesive layer.

Claim 18

A biosensor according to claim 15, wherein the conductivity through the adhesive layer is performed by a conductive material provided in a bored part formed in a double-sided adhesive tape.

Claim 19

A biosensor according to claim 15, wherein the conductivity through the adhesive layer is performed by a conductive material provided in a bored part formed in the adhesive layer.

Claim 20

A biosensor according to claim 15, wherein the conductivity through the adhesive layer is performed by a conductive material filled in bored parts formed in the adhesive layer provided on each of the bases.

Claim 21

A biosensor according to claim 15, wherein an oxidoreductase-electron transmitter mixture layer is provided on the working electrode.

Claim 22

A biosensor according to claim 15, wherein oxidoreductase-electron transmitter mixture layers are provided on the working electrode and the counter electrode.

Claim 23

A biosensor according to claim 15, further comprising a reference electrode fixed to one of the bases.

Claim 24

A biosensor according to claim 15, wherein one end of each of the bases has a tapered part, and a tip part of the working electrode or a tip part of the counter electrode is provided on each tapered part.

Claim 25

A biosensor according to claim 15, wherein the layer has a substantially straight edge for closing an inner edge of a space for sucking and housing a sample.

Claim 26

A biosensor comprising a longer upper base, a shorter lower base, a working electrode having an oxidoreductase fixed on the working electrode and a counter electrode, wherein the working electrode and the counter electrode are fixed on the longer upper base and the shorter lower base through an adhesive layer so as to have a facing structure, and lead parts for these electrodes are formed on the upper base, wherein the electrode on the upper base is conducted to its lead part through the upper base, and the electrode on the lower base is conducted to its lead part provided on the upper base through the adhesive layer.

Claim 27

A biosensor comprising a longer upper base, a shorter lower base, a working electrode having an oxidoreductase fixed on the working electrode and a counter electrode, wherein the working electrode and the counter electrode are fixed on the longer upper base and the shorter lower base through an adhesive layer so as to have a facing structure, and lead parts for these electrodes are formed on the upper base, wherein the electrode on the upper base is conducted to its lead part through the upper base, and the electrode on the lower base is conducted to its lead part provided on the upper base through the adhesive layer.

Claim 28

A biosensor according to claim 24, wherein the layer has a substantially straight edge for closing an inner edge of a space for sucking and housing a sample.

Claim 29

A biosensor which comprises a shorter upper base, a longer lower base, a working electrode, an oxidoreductase fixed on the working electrode, and a counter electrode, wherein the working electrode and the counter electrode are fixed on the longer lower base and the shorter upper base through a spacer so as to have a facing structure, and a lead part for each of the two electrodes is formed in a position on the lower base, wherein the electrode on the upper base is conducted to its lead part through the spacer.

Claim 30

A biosensor which comprises a shorter upper base, a longer lower base, a working electrode, an oxidoreductase fixed on the working electrode, and a counter electrode, wherein the working electrode and the counter electrode are fixed on the longer lower base and the shorter upper base through a spacer so as to have a facing structure, and a lead part for each of the two electrodes is formed in a position on the lower base, wherein the electrode on the upper base is conducted to its lead part through the spacer.

Claim 31

A biosensor according to claim 26, wherein the conductivity through the spacer is performed by a conductive material provided in a bored part formed in the spacer.

Claim 32

A biosensor according to claim 26, wherein one end of each of the two bases has a tapered part, and a tip part of the working electrode or a tip part of the counter electrode is provided on each tapered part.

Claim 33

A biosensor according to claim 26, wherein an oxidoreductase-electron transmitter mixture layer is provided on the working electrode.

Claim 34

A biosensor according to claim 26, wherein oxidoreductase-electron transmitter mixture layers are provided on the working electrode and the counter electrode.

Claim 35

A biosensor according to claim 26, further comprising a reference electrode fixed to at least one of the two bases.

Claim 36

A biosensor according to claim 31, wherein the conductivity through the spacer is performed by a conductive material filled in a bored part formed in the spacer.

Claim 37

A biosensor according to claim 31, wherein one end of each of the two bases has a tapered part, and a tip part of the working electrode or a tip part of the counter electrode is provided on each tapered part.

Claim 38

A biosensor according to claim 31 wherein an oxidoreductase-electron transmitter mixture layer is formed on the working electrode.

Claim 39

A biosensor according to claim 31 wherein oxidoreductase-electron transmitter mixture layers are formed on the working electrode and the counter electrode.

Claim 40

A biosensor according to claim 26, wherein the layer has a substantially straight edge for closing an inner edge of a space for sucking and housing a sample.

Claim 41

A biosensor which comprises a lower base, an upper base, a working electrode, an oxidoreductase on the working electrode and a counter electrode, wherein the working electrode and the counter electrode are fixed on the upper base and the lower base through a spacer so as to have a facing structure, and two lead parts for the two electrodes formed on the upper base, wherein the electrode on the upper base is conducted to its lead part through the upper base, and the electrode on the lower base is conducted to its lead part on the upper base through the spacer.

Claim 42

A biosensor which comprises a lower base, an upper base, a working electrode, an oxidoreductase on the working electrode and a counter electrode, wherein the working electrode and the counter electrode are fixed on the upper base and the lower base through a spacer so as to have a facing structure, and two lead parts for the two electrodes formed on the upper base, wherein the electrode on the upper base is conducted to its lead part through the upper base, and the electrode on the lower base is conducted to its lead part on the upper base through the spacer.

Claim 43

A biosensor according to claim 37, wherein the conductivity through the base is performed by a conductive material provided in a bored part formed in the base.

Claim 44

A biosensor device which comprises a device body having a connector part, a working electrode and a counter electrode, the connector part having input terminals, a reaction sensor member which is to be inserted into the connector part of the device body in such a manner as to be attachable and detachable, the reaction sensor member having an output terminal of the working electrode and an output terminal of the counter electrode so as to be electrically connected to the input terminals of the connector part when the reaction sensor member is inserted into the connector part of the device body, and wherein a reaction part is formed on at least the working electrode, the reaction sensor member further having a sensor insertion judging electrode, the connector part of the device body having two input terminals, wherein the sensor insertion judging electrode has an output terminal which makes contact with the two input terminals of the connector part, so that the device body is started when the output terminal of the sensor insertion judging electrode becomes in contact with the two input terminals.

Claim 45

A biosensor device which comprises a device body having a connector part, a working electrode and a counter electrode, the connector part having input terminals, a reaction sensor member which is to be inserted into the connector part of the device body in such a manner as to be attachable and detachable, the reaction sensor member having an output terminal of the working electrode and an output terminal of the counter electrode so as to be electrically connected to the input terminals of the connector part when the reaction sensor member is inserted into the connector part of the device body, and wherein a reaction part is formed on at least the working electrode, the reaction sensor member further having a sensor insertion judging electrode, the connector part of the device body having two input terminals, wherein the sensor insertion judging electrode has an output terminal which makes contact with the two input terminals of the connector part, so that the device body is started when the output terminal of the sensor insertion judging electrode becomes in contact with the two input terminals.

Claim 46

A biosensor device according to claim 39, further comprising a control part which judges sucking of a sample to be measured by a signal for laying the working electrode and the counter electrode into closed circuit by the sucking of the sample to the reaction part, starts to count residual time up to a preset measurement end, transmits a count signal to a display part provided on the device body and displays it.

Claim 47

A biosensor device according to claim 39, wherein the working electrode and the counter electrode are placed along a common plane in relation to the reaction sensor member.

Claim 48

A biosensor device according to claim 39, wherein the reaction part is formed as a layer made of an oxidoreductase and an electron transmitter in mixture.

Claim 49

A biosensor device according to claim 39 wherein a sample to be measured is sucked onto the reaction part in a state where a voltage is applied between the working electrode and the counter electrode after the sensor insertion, and a current value is generated by oxidation and then is taken out so as to be measured.