US6214979B1
Expired / Lapsed
US
⏱️ 0.0 years remaining
Homogeneous assay system
Assignee: Roche Molecular Systems Inc
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Filing: 1997-09-19
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Granted: 2001-04-10
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Expires: 2017-09-19
Abstract
Abstract A process of detecting a target nucleic acid using labeled oligonucleotides uses the 5' to 3' nuclease activity of a nucleic acid polymerase to cleave annealed labeled oligonucleotide from hybridized duplexes and release labeled oligonucleotide fragments for detection. This process is easily incorporated into a PCR amplification assay.
Inventors
- 👤 David H. Gelfand
- 👤 Pamela M. Holland
- 👤 Randall K. Saiki
- 👤 Robert M. Watson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 14:41
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6844; C12Q1/686; C07; C07H; C07H21/00; C12Q1/6813; C12Q1/6816; C12Q1/6818; C12Q1/6823; C12Q1/6827; C12Q1/70; C12Q1/701; C12Q1/702; C12Q1/703; Y; Y10; Y10S; Y10S435/00; Y10S435/805; Y10S435/81; Y10S436/00; Y10S436/815
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
111 words
A detectably labeled oligonucleotide probe, which probe is blocked at the 3′ terminus to prohibit polymerase catalyzed extension of said probe, wherein said blocking is achieved either by adding a chemical moiety to the 3′ hydroxyl of the terminal nucleotide, which chemical moiety does not also serve as a label for subsequent detection, or by removing said 3′ hydroxyl; and wherein said labeled oligonucleotide probe comprises a pair of non-radioactive interactive labels consisting of a first label and a second label, said first label and second label attached to said oligonucleotide directly or indirectly, and wherein said first label is separated from said second label by a nuclease susceptible cleavage site.
Claim 2
Dependent Claim
111 words
A detectably labeled oligonucleotide probe, which probe is blocked at the 3′ terminus to prohibit polymerase catalyzed extension of said probe, wherein said blocking is achieved either by adding a chemical moiety to the 3′ hydroxyl of the terminal nucleotide, which chemical moiety does not also serve as a label for subsequent detection, or by removing said 3′ hydroxyl; and wherein said labeled oligonucleotide probe comprises a pair of non-radioactive interactive labels consisting of a first label and a second label, said first label and second label attached to said oligonucleotide directly or indirectly, and wherein said first label is separated from said second label by a nuclease susceptible cleavage site.
Claim 3
Dependent Claim
27 words
The detectably labeled oligonucleotide probe of claim 1 where in said first label is at the 5′ terminus and said second label is at the 3′ terminus.
Claim 4
Dependent Claim
33 words
The detectably labeled oligonucleotide probe of claim 1 wherein said first and second labels comprise a pair of interactive signal-generating labels positioned on said labeled oligonucleotide to quench the generation of detectable signal.
Claim 5
Dependent Claim
25 words
The detectably labeled oligonucleotide probe of claim 3 wherein said first label is a fluorophore and said second label is a quencher which interacts therewith.
References Cited (Backward Citations)
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2004-04-09 | 2004-10-09 | 2005-04-10 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2008-04-09 | 2008-10-09 | 2009-04-10 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2012-04-09 | 2012-10-09 | 2013-04-10 | $7700.00 | Window Closed / Lapsed |