US6270637B1
Expired / Lapsed
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Electrochemical biosensor test strip
Assignee: Roche Diabetes Care Inc
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Filing: 1999-08-31
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Granted: 2001-08-07
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Expires: 2019-08-31
Abstract
Abstract An electrochemical biosensor test strip with four new features. The test strip includes an indentation for tactile feel as to the location of the strips sample application port. The sample application port leads to a capillary test chamber, which includes a test reagent. The wet reagent includes from about 0.2% by weight to about 2% by weight polyethylene oxide from about 100 kilodaltons to about 900 kilodaltons mean molecular weight, which makes the dried reagent more hydrophilic and sturdier to strip processing steps, such as mechanical punching, and to mechanical manipulation by the test strip user. The roof of the capillary test chamber includes a transparent or translucent window which operates as a "fill to here" line, thereby identifying when enough test sample (a liquid sample, such as blood) has been added to the test chamber to accurately perform a test. The test strip may further include a notch located at the sample application port. The notch reduces a phenomenon called "dose hesitation".
Inventors
- 👤 William F. Crismore
- 👤 Nigel A. Surridge
- 👤 Daniel R. McMinn
- 👤 Richard J. Bodensteiner
- 👤 Eric R. Diebold
- 👤 R. Dale Delk
- 👤 David W. Burke
- 👤 Jiaxiong Jason Ho
- 👤 Robert Kitchel Earl
- 👤 Brian A. Heald
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 07:50
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/001; G; G01; G01N; G01N27/00; G01N27/26; G01N27/28; G01N27/30; G01N27/327; G01N27/3271; G01N27/3272; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54366; G01N33/54373; G01N33/5438; Y; Y10; Y10S; Y10S435/00; Y10S435/817
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
76 words
An electrochemical test strip comprising electrodes; and a reagent comprising reaction components appropriate for performing a test, and a dissolvable or suspendable film forming mixture including polyethylene oxide having a mean molecular weight from about 100 kilodaltons to about 900 kilodaltons, wherein the reagent is applied to the test strip in a wet form, and subsequently dried, and thereby adapted to be redissolved or resuspended upon addition of an aqueous test sample to the dried reagent.
Claim 2
Dependent Claim
76 words
An electrochemical test strip comprising electrodes; and a reagent comprising reaction components appropriate for performing a test, and a dissolvable or suspendable film forming mixture including polyethylene oxide having a mean molecular weight from about 100 kilodaltons to about 900 kilodaltons, wherein the reagent is applied to the test strip in a wet form, and subsequently dried, and thereby adapted to be redissolved or resuspended upon addition of an aqueous test sample to the dried reagent.
Claim 3
Dependent Claim
21 words
The electrochemical test strip of claim 1 , wherein the mean molecular weight of polyethylene oxide is about 300 kilo Daltons.
Claim 4
Dependent Claim
28 words
The electrochemical test strip of claim 1 , wherein the reaction components appropriate for performing the test include an enzyme quinoprotein glucose dehydrogenase and a redox mediator ferricyanide.
Claim 5
Dependent Claim
28 words
The electrochemical test strip of claim 1 , wherein the reaction components appropriate for performing a test include an enzyme quinoprotein glucose dehydrogenase and a redox mediator ferricyanide.
Claim 6
Dependent Claim
28 words
The electrochemical test strip of claim 4 , wherein the enzyme quinoprotein glucose dehydrogenase comprises a stabilized association of coenzyme PQQ and the apoenzyme of quinoprotein glucose dehydrogenase.
Claim 7
Dependent Claim
20 words
The electrochemical test strip of claim 5 , wherein the apoenzyme of quinoprotein glucose dehydrogenase is obtained from Acinetobacter Calcoaceticus.
Claim 8
Dependent Claim
31 words
The electrochemical test strip of claim 1 , wherein the mixture is filtered by passage through a 100 micron sieve bag or a 100 micron filter integral to a pumping system.
Claim 9
Dependent Claim
52 words
An electrochemical test strip comprising electrodes; and a reagent comprising reaction components appropriate for performing a test and polyethylene oxide having a mean molecular weight from about 100 kilodaltons to about 900 kilodaltons, wherein the reagent is adapted to redissolve or resuspend upon addition of an aqueous test sample to the reagent.
Claim 10
Dependent Claim
52 words
An electrochemical test strip comprising electrodes; and a reagent comprising reaction components appropriate for performing a test and polyethylene oxide having a mean molecular weight from about 100 kilodaltons to about 900 kilodaltons, wherein the reagent is adapted to redissolve or resuspend upon addition of an aqueous test sample to the reagent.
Claim 11
Dependent Claim
21 words
The electrochemical test strip of claim 8 , wherein the mean molecular weight of polyethylene oxide is about 300 kilo Daltons.
Claim 12
Dependent Claim
19 words
The electrochemical test strip of claim 8 , wherein the dried reagent comprises about 6.2% polyethylene oxide by weight.
Claim 13
Dependent Claim
27 words
The electrochemical test strip of claim 8 , wherein the dried reagent comprises from about 2,000 to about 9,000 units of enzyme activity per gram of reagent.
Claim 14
Dependent Claim
28 words
The electrochemical test strip of claim 8 , wherein the reaction components appropriate for performing a test include an enzyme quinoprotein glucose dehydrogenase and a redox mediator ferricyanide.
Claim 15
Dependent Claim
43 words
The electrochemical test strip of claim 8 , wherein a reaction component is selected from the group consisting of enzyme quinoprotein glucose dehydrogenase, polyethylene oxide, microcrystalline hydroxyethylcellulose, microcrystalline cellulose, monobasic potassium phosphate, dibasic potassium phosphate, potassium ferricyanide, sodium succinate, trehalose, and a surfactant.
Claim 16
Dependent Claim
28 words
The electrochemical test strip of claim 13 , wherein the enzyme quinoprotein glucose dehydrogenase comprises a stabilized association of coenzyme PQQ and the apoenzyme of quinoprotein glucose dehydrogenase.
Claim 17
Dependent Claim
20 words
The electrochemical test strip of claim 14 , wherein the apoenzyme of quinoprotein glucose dehydrogenase is obtained from Acinetobacter Calcoaceticus.
References Cited (Backward Citations)
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| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 2004-08-06 | 2005-02-05 | 2005-08-07 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2008-08-06 | 2009-02-05 | 2009-08-07 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2012-08-06 | 2013-02-05 | 2013-08-07 | $7700.00 | Window Closed / Lapsed |