US6471839B1
Expired / Lapsed
US
⏱️ 0.0 years remaining
Biosensor
Assignee: Panasonic Holdings Corp
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Filing: 2000-05-17
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Granted: 2002-10-29
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Expires: 2020-05-17
Abstract
Abstract The present invention provides a biosensor which enables quick measurement and is excellent in storage characteristic. This biosensor comprises an electrically insulating base plate on which an electrode system having at least a measuring electrode and a counter electrode is formed, a cover member which is integrated to the base plate so as to form a sample solution supply path for supplying a sample solution to the electrode system between the cover member and the base plate, and a carrier composed of fiber supporting a reagent containing at least an oxidoreductase, and the carrier is placed in the sample solution supply path. Preferably, the carrier is constituted at least of two carrier pieces and each carrier piece supports a different reagent.
Inventors
- 👤 Tomohiro Yamamoto
- 👤 Toshihiko Yoshioka
- 👤 Shiro Nankai
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 14:10
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/001; C12Q1/004; C12Q1/005
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
118 words
A biosensor comprising an electrically insulating base plate, an electrode system having at least a measuring electrode and a counter electrode formed on said base plate, a cover member which is integrated to said base plate so as to form a sample solution supply path for supplying a sample solution to said electrode system between said cover member and said base plate, and a carrier comprising at least two carrier pieces, each piece comprising a fiber supporting a reagent, a first piece supporting at least an oxidoreductase and at least a second piece supporting a different reagent, wherein said carrier is placed in said sample solution supply path, in the vicinity of, but not contacting, the electrode system.
Claim 2
Dependent Claim
118 words
A biosensor comprising an electrically insulating base plate, an electrode system having at least a measuring electrode and a counter electrode formed on said base plate, a cover member which is integrated to said base plate so as to form a sample solution supply path for supplying a sample solution to said electrode system between said cover member and said base plate, and a carrier comprising at least two carrier pieces, each piece comprising a fiber supporting a reagent, a first piece supporting at least an oxidoreductase and at least a second piece supporting a different reagent, wherein said carrier is placed in said sample solution supply path, in the vicinity of, but not contacting, the electrode system.
Claim 3
Dependent Claim
22 words
The biosensor in accordance with claim 1 , wherein said carrier is fixed in said sample solution supply path by an adhesive.
Claim 4
Dependent Claim
123 words
A biosensor comprising an electrically insulating base plate, an electrode system having at least a measuring electrode and a counter electrode formed on said base plate, a cover member which is integrated to said base plate so as to form a sample solution supply path for supplying a sample solution to said electrode system between said cover member and said base plate, and a carrier composed of fiber supporting a reagent containing at least an oxidoreductase, wherein said carrier is placed in said sample solution supply path and wherein said carrier is fixed in said sample solution supply path by an adhesive, further wherein said reagent contains at least cholesterol oxidase, cholesterol esterase and an electron mediator and said adhesive is a surfactant.
Claim 5
Dependent Claim
123 words
A biosensor comprising an electrically insulating base plate, an electrode system having at least a measuring electrode and a counter electrode formed on said base plate, a cover member which is integrated to said base plate so as to form a sample solution supply path for supplying a sample solution to said electrode system between said cover member and said base plate, and a carrier composed of fiber supporting a reagent containing at least an oxidoreductase, wherein said carrier is placed in said sample solution supply path and wherein said carrier is fixed in said sample solution supply path by an adhesive, further wherein said reagent contains at least cholesterol oxidase, cholesterol esterase and an electron mediator and said adhesive is a surfactant.
Claim 6
Dependent Claim
136 words
A biosensor comprising an electrically insulating base plate, an electrode system having at least a measuring electrode and a counter electrode formed on said base plate, a cover member which is integrated to said base plate so as to form a sample solution supply path for supplying a sample solution to said electrode system between said cover member and said base plate, and a carrier comprising at least two carrier pieces, each piece comprising a fiber supporting a reagent, a first piece supporting at least an oxidoreductase and at least a second piece supporting a different reagent, wherein said carrier is placed in said sample solution supply path, wherein said different reagent contains at least cholesterol oxidase, cholesterol esterase and an electron mediator, and the oxidoreductase and the electron mediator are supported on separate carrier pieces.
Claim 7
Dependent Claim
136 words
A biosensor comprising an electrically insulating base plate, an electrode system having at least a measuring electrode and a counter electrode formed on said base plate, a cover member which is integrated to said base plate so as to form a sample solution supply path for supplying a sample solution to said electrode system between said cover member and said base plate, and a carrier comprising at least two carrier pieces, each piece comprising a fiber supporting a reagent, a first piece supporting at least an oxidoreductase and at least a second piece supporting a different reagent, wherein said carrier is placed in said sample solution supply path, wherein said different reagent contains at least cholesterol oxidase, cholesterol esterase and an electron mediator, and the oxidoreductase and the electron mediator are supported on separate carrier pieces.
Claim 8
Dependent Claim
136 words
A biosensor comprising an electrically insulating base plate, an electrode system having at least a measuring electrode and a counter electrode formed on said base plate, a cover member which is integrated to said base plate so as to form a sample solution supply path for supplying a sample solution to said electrode system between said cover member and said base plate, and a carrier comprising at least two carrier pieces, each piece comprising a fiber supporting a reagent, a first piece supporting at least an oxidoreductase and at least a second piece supporting a different reagent, wherein said carrier is placed in said sample solution supply path, wherein said different reagent comprises at least cholesterol oxidase, cholesterol esterase, a surfactant and an electron mediator, and the carrier piece containing the cholesterol esterase contains the surfactant.
Claim 9
Dependent Claim
136 words
A biosensor comprising an electrically insulating base plate, an electrode system having at least a measuring electrode and a counter electrode formed on said base plate, a cover member which is integrated to said base plate so as to form a sample solution supply path for supplying a sample solution to said electrode system between said cover member and said base plate, and a carrier comprising at least two carrier pieces, each piece comprising a fiber supporting a reagent, a first piece supporting at least an oxidoreductase and at least a second piece supporting a different reagent, wherein said carrier is placed in said sample solution supply path, wherein said different reagent comprises at least cholesterol oxidase, cholesterol esterase, a surfactant and an electron mediator, and the carrier piece containing the cholesterol esterase contains the surfactant.
Claim 10
Dependent Claim
48 words
The biosensor in accordance with claim 5 , wherein said reagent contains at least cholesterol oxidase, cholesterol esterase, a surfactant and an electron mediator, and wherein said electrode system comprises silver and a carbon layer covering the silver, and said electrode system is coated with a hydrophilic polymer.
Claim 11
Dependent Claim
73 words
A biosensor comprising an electrically insulating base plate, an electrode system having at least a measuring electrode and a counter electrode formed on said base plate, and a carrier composed of fiber supporting a reagent containing at least an oxidoreductase, the carrier comprising at least two carrier pieces, each carrier piece supporting a different reagent, wherein said carrier is fixed by an adhesive in the vicinity of, but not contacting, said electrode system.
Claim 12
Dependent Claim
73 words
A biosensor comprising an electrically insulating base plate, an electrode system having at least a measuring electrode and a counter electrode formed on said base plate, and a carrier composed of fiber supporting a reagent containing at least an oxidoreductase, the carrier comprising at least two carrier pieces, each carrier piece supporting a different reagent, wherein said carrier is fixed by an adhesive in the vicinity of, but not contacting, said electrode system.
Claim 13
Dependent Claim
75 words
A biosensor comprising an electrically insulating base plate, an electrode system having at least a measuring electrode and a counter electrode formed on said base plate, and a carrier composed of fiber supporting a reagent containing at least an oxidoreductase, wherein said carrier is fixed by an adhesive in the vicinity of said electrode system, wherein said reagent contains at least cholesterol oxidase, cholesterol esterase and an electron mediator, and said adhesive is a surfactant.
Claim 14
Dependent Claim
75 words
A biosensor comprising an electrically insulating base plate, an electrode system having at least a measuring electrode and a counter electrode formed on said base plate, and a carrier composed of fiber supporting a reagent containing at least an oxidoreductase, wherein said carrier is fixed by an adhesive in the vicinity of said electrode system, wherein said reagent contains at least cholesterol oxidase, cholesterol esterase and an electron mediator, and said adhesive is a surfactant.
Claim 15
Dependent Claim
30 words
The biosensor in accordance with claim 8 , wherein said electrode system comprises silver and a,carbon layer covering the silver, and said electrode system is coated with a hydrophilic polymer.
Claim 16
Dependent Claim
82 words
A biosensor comprising an electrically insulating base plate, an electrode system having at least a measuring electrode and a counter electrode formed on said base plate, and a carrier composed of fiber supporting a reagent containing at least an oxidoreductase, wherein said carrier is fixed by an adhesive in the vicinity of said electrode system, wherein said reagent contains at least cholesterol oxidase, cholesterol esterase and an electron mediator, and the oxidoreductase and the electron mediator are supported on separate carrier pieces.
Claim 17
Dependent Claim
82 words
A biosensor comprising an electrically insulating base plate, an electrode system having at least a measuring electrode and a counter electrode formed on said base plate, and a carrier composed of fiber supporting a reagent containing at least an oxidoreductase, wherein said carrier is fixed by an adhesive in the vicinity of said electrode system, wherein said reagent contains at least cholesterol oxidase, cholesterol esterase and an electron mediator, and the oxidoreductase and the electron mediator are supported on separate carrier pieces.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2005-10-28 | 2006-04-29 | 2006-10-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2009-10-28 | 2010-04-29 | 2010-10-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2013-10-28 | 2014-04-29 | 2014-10-29 | $7700.00 | Window Closed / Lapsed |