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EP0036274A2
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EP
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Methods of monitoring micro-organisms and media for culture
Assignee: Japan Tectron Instruments Corp
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Filing: 1981-03-05
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Granted: —
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Expires: 2001-03-05
Abstract
Abstract A method of monitoring and identifying micro-organism by measuring impedance change of a supporting medium on which micro-organism is growth. Impedance change includes decrease and increase in a peculiar pattern characteristic of micro-organism as the latter achieves growth. The supporting medium is constituted by a basal medium including hydrolyzate of protein and added with carbohydrate and salt and if necessary, aminoacid.
Inventors
- 👤 Hidehiko Fujioka
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-31 10:43
CPC Classifications
C; C12; C12M; C12M41/00; C12M41/30; C12M41/36; G; G01; G01N; G01N33/00; G01N33/48; G01N33/483; G01N33/487; G01N33/48707; G01N33/48735
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
57 words
A method of monitoring micro-organism, comprising steps of preparing a supporting medium starting from a basal medium which contains hydrolyzates of proteins as main components by adding carbohydrates and salts to said basal medium, cultivating micro-organism on said supporting medium, and measuring changes in impedance of said supporting medium, said change including decrease and increase in impedance.
Claim 2
Independent Claim
57 words
A method of monitoring micro-organism, comprising steps of preparing a supporting medium starting from a basal medium which contains hydrolyzates of proteins as main components by adding carbohydrates and salts to said basal medium, cultivating micro-organism on said supporting medium, and measuring changes in impedance of said supporting medium, said change including decrease and increase in impedance.
Claim 3
Dependent Claim
18 words
A method of monitoring micro-organism as set forth in claim 1, wherein said supporting medium further includes aminoacids.
Claim 4
Dependent Claim
73 words
A method of monitoring micro-organism as set forth in claim 1, wherein parameters which include rate of impedance decrease for a unit time, rate of impedance increase for a unit time, a period during which iimpedance is decreased, a period during which impedance is decreased and rate of impedance decrease immediately before said impedance increase occurs are measured for said supporting medium and compared with corresponding known parameters to thereby identify said micro-organism.
Claim 5
Dependent Claim
31 words
A method of monitoring micro-organism as set forth in claim 1 or 2, wherein difference in utilization of different carbohydrates by micro-organism is detected as impedance change for identifying said micro-organism.
Claim 6
Dependent Claim
33 words
A method of monitoring micro-organism as set forth in claim 1 or 2, wherein difference in decarboxylation of different aminoacids brought about by micro-organism is detected as impedance change for identifying said micro-organism.
Claim 7
Independent Claim
33 words
A medium for use in impedametrical monitoring of micro-organism, comprising a basal medium containing hydrolyzates of proteins as main components, said basal medium further being added with at least carbo-_ hydrates and salts.
Claim 8
Independent Claim
33 words
A medium for use in impedametrical monitoring of micro-organism, comprising a basal medium containing hydrolyzates of proteins as main components, said basal medium further being added with at least carbo-_ hydrates and salts.
Claim 9
Independent Claim
20 words
A medium for use in impedametrical monitoring of micro-organism as set forth in claim ε, said medium further includes aminoacids.
Claim 10
Dependent Claim
33 words
medium for use in impedametrical monitoring of micro-organism as set forth in claim 6, said basal medium is added with 2.5 to 10 gr/l of carbohydrates and 1.0 to 10 gr/l of salts.
Claim 11
Dependent Claim
55 words
A medium for use in impedametrical monitoring of micro-organism as set forth in claim 6 or 7 or 8, wherein said carbohydrates include one selected from a group consisting of monosaccharides such as glucose, mannitol and the like, disaccharides such as sucrose, melibiose and the like and polysaccharides such as raffinose, melezitose and the like.
Claim 12
Dependent Claim
44 words
A medium for use in impedametrical monitoring of micro-organism as set forth in claim 6, 7 or 8, wherein said salts include one or more components selected from a group consisting of inorganic strong electrolytes such as salts of sodium, potassium and the like.
Claim 13
Dependent Claim
23 words
A medium for use in impedametrical monitoring of micro-organism as set forth in claim 10, wherein salt of sodium is disodium hydrogen phosphate.
Claim 14
Dependent Claim
29 words
A medium for use in impedametrical monitoring of micro-organism as set forth in claim 6, wherein aminoacids includes one selected from a group consisting of alginine, lysine and ornithine.
References Cited (Backward Citations)
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