Patent EP0046039B1 successfully refreshed from Google Patents.
EP0046039B1
Expired / Lapsed
EP
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Synthetic urogastrone gene, corresponding plasmid recombinants, transformed cells, production thereof and urogastrone expression
Assignee: GD Searle LLC
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Filing: 1981-07-31
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Granted: 1987-01-14
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Expires: 2001-07-31
Abstract
No abstract record available.
Inventors
- 👤 Michael Anthony William Eaton
- 👤 John Craig Smith
- 👤 Michael Terence Doel
- 👤 David Malcolm James Lilley
- 👤 Norman Henry Carey
- 👤 Leslie David Bell
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-30 14:02
CPC Classifications
C; C07; C07H; C07H21/00; C07K; C07K14/00; C07K14/435; C07K14/475; C07K14/485; C12; C12N; C12N15/00; C12N15/09; C12N15/11; C12N15/62; C12N15/63; C12N15/70; C07K2319/00; C07K2319/70; C07K2319/74; C07K2319/75; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/848; Y10S435/849; Y10S930/00; Y10S930/01; Y10S930/12
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
34 words
A synthetic gene characterised in that it codes for the expression of urogastrone or an equivalent thereof or a sub-unit thereof in a bacterial cell and comprises the following sequence or a sub-unit thereof:
Claim 2
Dependent Claim
34 words
A synthetic gene characterised in that it codes for the expression of urogastrone or an equivalent thereof or a sub-unit thereof in a bacterial cell and comprises the following sequence or a sub-unit thereof:
Claim 3
Dependent Claim
20 words
A synthetic gene as claimed in Claim 1 characterised in that it comprises the following sequence or a sub-unit thereof:
Claim 4
Dependent Claim
36 words
A process for the production of a synthetic gene or a sub-unit thereof as claimed in Claim 1 or Claim 2 characterised in that it comprises the assembly and ligation of a number of oligoncleotide blocks.
Claim 5
Dependent Claim
80 words
A plasmid recombinant characterised in that it comprises a plasmid vector having inserted therein at an appropriate insertion site a synthetic gene or a sub-unit thereof as claimed in Claim 1 or Claim 2, the plasmid recombinant enabling translation in the correct phase for the mRNA corresponding to the inserted gene or sub-unit thereof and having a bacterial promoter upstream of and adjacent to the insertion site such that the inserted gene or sub-unit thereof is under bacterial promoter control.
Claim 6
Dependent Claim
18 words
A plasmid recombinant as claimed in Claim 4 characterised in that it comprises as the plasmid vector pLFI.
Claim 7
Dependent Claim
45 words
A process for the production of a plasmid recombinant as claimed in Claim 4 characterised in that it comprises inserting a synthetic gene or a sub-unit thereof as claimed in Claim 1 or Claim 2 into an appropriate insertion site of an appropriate plasmid vector.
Claim 8
Dependent Claim
21 words
A bacterial cell characterised in that it comprises inserted therein a plasmid recombinant as claimed in Claim 4 or Claim 5.
Claim 9
Dependent Claim
35 words
A process for the production of a bacterial cell as claimed in Claim 7 characterised in that it comprises inserting a plasmid recombinant as claimed in Claim 4 or Claim 5 into a bacterial cell.
Claim 10
Dependent Claim
33 words
A process for the production of urogastrone or an equivalent thereof or a sub-unit thereof characterised in that it comprises culturing a bacterial cell as claimed in Claim 7 and recovering expressed protein.
Claim 11
Dependent Claim
50 words
A fused polypeptide characterised in that it comprises urogastrone or an equivalent thereof or a sub-unit thereof covalently bonded with all or part of a gene of the tryptophan operon and in that it is obtained by culturing a bacterial cell as claimed in Claim 7 and recovering expressed protein.
Claim 12
Dependent Claim
20 words
A fused polypeptide as claimed in Claim 10 wherein the gene of the tryptophan operon is the trp E gene.
References Cited (Backward Citations)
No citation records indexed.
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