Abstract In a method for fluorescence spectroscopic determination of a biological substance provided with a marker consisting of a lantanide chelate complex formed by a lantanide coupled to the substance via a chelate forming compound, such as an EDTA-analogue, the lantanide ion or the complete chelate before the detection is split from the active substance for instance by adding a buffer with a pH-value below 3.5 and a splitting detergent, whereafter the excitation in the determination takes place in the solution by using a short radiation pulse and the fluorescence from the marker is detected when the fluorescence from the noise sources has substantially ceased.
Method for fluorescence spectroscopic determination of a biological substance provided with a marker consisting of a lanthanide chelate complex formed of a lanthanide coupled to the substance via a chelate forming compound, characterized in that the lanthanide is separated from the substance by addition of a solution having a low pH and comprising a detergent, whereupon the determination is carried out in the solution.
Claim 2
Dependent Claim
64 words
Method for fluorescence spectroscopic determination of a biological substance provided with a marker consisting of a lanthanide chelate complex formed of a lanthanide coupled to the substance via a chelate forming compound, characterized in that the lanthanide is separated from the substance by addition of a solution having a low pH and comprising a detergent, whereupon the determination is carried out in the solution.
Claim 3
Dependent Claim
13 words
Method according to claim 1, characterized in that the solution comprises a β-diketone.
Claim 4
Dependent Claim
16 words
Method according to claims 1 or 2, characterized in that the solution comprises a Lewis base.
Claim 5
Dependent Claim
12 words
Method according to claim 3, characterized in that the base is trioctylphosphineoxide.
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