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EP0106685A2
Expired / Lapsed
EP
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Fluorescence screening for blood typing
Assignee: Syva Co
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Filing: 1983-10-14
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Granted: —
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Expires: 2003-10-14
Abstract
Abstract A sensitive method for identifying red blood cell antigens or antibodies thereto is provided. A fluorescent bead is conjugated to a receptor specific for a red blood cell antigen (ligand) or alternatively with an antigen for determination of antibodies. The conjugate is mixed with a red blood cell containing composition. A change in fluorescence compared with a control reveals the presence or absence of the antigen or antibody.
Inventors
- 👤 Yen-Ping Liu
- 👤 Edwin F. Ullman
- 👤 Martin J. Becker
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-01 15:57
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/80; Y; Y10; Y10S; Y10S436/00; Y10S436/827
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
122 words
A method for determining in a sample the presence of a surface antigen of red blood cells bound to said cells or the presence of antibodies to such surface antigen, which comprises: combining said sample with fluorescent particles conjugated with receptor homologous to said surface antigen or conjugated with surface antigen for said antibodies: wherein for the determination of the surface antigen bound to said red blood cells only receptor conjugate is employed, and for the determination of said antibodies: (a) antigen conjugate is employed in combination with red blood cells having the same antigen or (b) receptor conjugate is employed; and determining the change in fluorescence as compared to a sample having a known red blood cell antigen or antibody thereto.
Claim 2
Independent Claim
122 words
A method for determining in a sample the presence of a surface antigen of red blood cells bound to said cells or the presence of antibodies to such surface antigen, which comprises: combining said sample with fluorescent particles conjugated with receptor homologous to said surface antigen or conjugated with surface antigen for said antibodies: wherein for the determination of the surface antigen bound to said red blood cells only receptor conjugate is employed, and for the determination of said antibodies: (a) antigen conjugate is employed in combination with red blood cells having the same antigen or (b) receptor conjugate is employed; and determining the change in fluorescence as compared to a sample having a known red blood cell antigen or antibody thereto.
Claim 3
Dependent Claim
20 words
A method according to Claim 1 wherein said surface antigen is selected from the group consisting of A and B.
Claim 4
Dependent Claim
13 words
A method according to Claim 1, wherein said surface antigen is Rh factor.
Claim 5
Dependent Claim
19 words
A method according to Claim 1 wherein said receptor is an antibody to a red blood cell surface antigen.
Claim 6
Dependent Claim
13 words
A method according to Claim 4 wherein said antibody is a monoclonal antibody.
Claim 7
Dependent Claim
12 words
A method according to Claim 1 wherein said receptor is a lectin.
Claim 8
Dependent Claim
14 words
A method according to Claim 1 wherein said fluorescent particle is a latex particle.
Claim 9
Dependent Claim
17 words
A method according to Claim 1, wherein said receptor conjugate is employed for the detection of antibodies.
References Cited (Backward Citations)
No citation records indexed.
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