Patent EP0136362B1 successfully refreshed from Google Patents.
EP0136362B1
Expired / Lapsed
EP
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Biosensor
Assignee: Panasonic Holdings Corp
•
Filing: 1984-03-06
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Granted: 1990-12-19
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Expires: 2004-03-06
Abstract
Abstract A measuring electrode (27) and an adjacent electrode (28) are embedded in an insulating substrate (26). The exposed surface of the electrode system is coated with a porous substance (29) carrying an oxidation reductase (30) and, if needed, an electron acceptor (31) in a dry state, to form a biosensor. A predetermined voltage is applied between the electrodes (27, 28) of the biosensor, and a matrix-containing sample is dropped on the porous substance (29). A current proportional to the substrate concentration flows, so it is possible to quantitatively determine the matrix concentration of various biological samples highly accurately, rapidly and easily.
Inventors
- 👤 Mariko Kawaguri
- 👤 Shiro Nankai
- 👤 Takashi Iijima
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-07 08:17
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/001; C12Q1/005; C12Q1/004
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
121 words
A bio-sensor for measuring the substrate concentration of a liquid sample, comprising an insulating substrate plate (19; 26; 32; 36) provided with an electrode system containing at least a working electrode (20; 27; 33; 37) and a counter electrode (21; 28; 34; 38), the electrode system being covered by a substrate (23; 29; 39) permeable to liquid and containing an enzyme (24; 30; 40) capable of inducing a substrate reaction which is electrochemically detectable by means of the electrode system, characterized in that the substrate (23; 29; 39) is porous and contains an oxidoreductase (24; 30; 40), the sensor further containing an electrode acceptor (25; 31; 41), and both the oxidoreductase and the electron acceptor being soluble in the liquid sample.
Claim 2
Dependent Claim
121 words
A bio-sensor for measuring the substrate concentration of a liquid sample, comprising an insulating substrate plate (19; 26; 32; 36) provided with an electrode system containing at least a working electrode (20; 27; 33; 37) and a counter electrode (21; 28; 34; 38), the electrode system being covered by a substrate (23; 29; 39) permeable to liquid and containing an enzyme (24; 30; 40) capable of inducing a substrate reaction which is electrochemically detectable by means of the electrode system, characterized in that the substrate (23; 29; 39) is porous and contains an oxidoreductase (24; 30; 40), the sensor further containing an electrode acceptor (25; 31; 41), and both the oxidoreductase and the electron acceptor being soluble in the liquid sample.
Claim 3
Dependent Claim
29 words
A bio-sensor according to claim 1, characterized in that the enzyme (24; 30; 40) and/or the electron acceptor (25; 31; 41) is in the form of finely divided particles.
Claim 4
Dependent Claim
25 words
A bio-sensor according to claim 1, characterized in that said porous substrate carries both said oxidoreductase (24; 30; 40) and electron acceptor (25; 31; 41).
Claim 5
Dependent Claim
31 words
A bio-sensor according to claim 1, characterized in that said working electrode (20; 27; 33; 37) comprises platinum and said counter electrode (21; 28; 34; 38) comprises platinum or silver/silver chloride.
Claim 6
Dependent Claim
38 words
A bio - sensor according to claim 1, characterized in that said electrode system is composed of a working electrode (20; 27; 33; 37), a counter electrode (21; 28; 34; 38) and a reference electrode (22; 35; 43).
Claim 7
Dependent Claim
17 words
A bio-sensor according to claim 1, characterized in that said porous substrate comprises a hydrophilic porous membrane.
Claim 8
Dependent Claim
14 words
A bio-sensor according to claim 1, characterized in that said electron acceptor is oxygen.
Claim 9
Dependent Claim
32 words
A bio-sensor according to claim 1, characterized in that said enzyme (24; 30; 40) is one member selected from the group consisting of glucose oxidase, alcohol oxidase, xanthine oxidase and cholesterol oxidase.
Claim 10
Dependent Claim
27 words
A bio-sensor according to claim 1, characterized in that, as said electron acceptor, an oxidizing dye (25; 31; 41) working in conjugation with said oxidoreductase, is used.
Claim 11
Dependent Claim
43 words
A bio-sensor according to claim 9, characterized in that, as said oxidizing dye (25; 31; 41) working in conjugation with the oxidoreductase, one member selected from the group consisting of potassium ferricyanide, p-benzoquinone, 2,6-dichlorophenolindophenol, methylene blue, phenazine methosulfate and potassium (3-naphthoquinone-4-sulfonate is used.
Claim 12
Dependent Claim
21 words
A bio-sensor according to claim 9, characterized in that said oxidoreductase is glucose oxidase and said electron acceptor is potassium ferricyanide.
Claim 13
Dependent Claim
23 words
A bio-sensor according to claim 6, characterized in that at least the oxidoreductase is carried on said porous membrane in a dry state.
Claim 14
Dependent Claim
23 words
A bio-sensor according to claim 6, characterized in that said porous membrane (42) has a pore diameter of not more than 3 pm.
Claim 15
Dependent Claim
20 words
A bio-sensor according to claim 6, characterized in that said porous substrate was previously treated with a surface active agent.
Claim 16
Dependent Claim
18 words
A bio-sensor according to claim 6, characterized in that an anticoagulant is carried on said porous membrane (42).
References Cited (Backward Citations)
No citation records indexed.
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