Patent EP0155360A1 successfully refreshed from Google Patents.
EP0155360A1
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Method for identifying and characterizing organisms
Assignee: WEBSTER, JOHN A., JR.
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Filing: 1982-09-24
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Granted: —
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Expires: 2002-09-24
Abstract
Abstract A method of detecting a prokaryotic organism comprises selectively hybridising the DNA of the prokaryotic organism with a detectably labeled prokaryotic ribosomal RNA-information-containing hybridisation probe.
Inventors
- 👤 John A. Webster, Jr.
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-01 14:58
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6876; C12Q1/6888; C12Q1/689; C12Q1/6813; C12Q1/6827; C12Q1/683
Claims Analysis
Total Claims: 27
Claim 1
Independent Claim
27 words
A method of detecting a prokaryotic organism, which method comprises selectively hybridising the DNA of the prokaryotic organism with. a detectably labeled prokayotic ribosomal RNA-information-containing hybridisation probe.
Claim 2
Independent Claim
27 words
A method of detecting a prokaryotic organism, which method comprises selectively hybridising the DNA of the prokaryotic organism with. a detectably labeled prokayotic ribosomal RNA-information-containing hybridisation probe.
Claim 3
Dependent Claim
22 words
A method as claimed in Claim 1, wherein the prokaryotic organism is in the presence of or associated with a eukaryotic organism.
Claim 4
Dependent Claim
21 words
A method as claimed in Claim 1 or 2, wherein said ribosomal RNA information-containing nucleic acid is radiolabeled or metal labeled.
Claim 5
Dependent Claim
20 words
A method as claimed in Claim 1, 2 or 3, wherein said ribosomal RNA information-containing hybridisation probe is ribosomal RNA.
Claim 6
Dependent Claim
23 words
A method as claimed in Claim 1, 2 or 3, wherein said ribosomal RNA information-containing hybridisation probe is DNA complementary to ribosomal RNA.
Claim 7
Dependent Claim
29 words
A method as claimed in Claim 1, 2 or 3, wherein said ribosomal RNA information-containing hybridisation probe is DNA obtained by nick-translating or cloning DNA complementary to ribosomal RNA.
Claim 8
Independent Claim
31 words
A method as claimed in any one of Claims 1 to 6, wherein said unknown organism being detected is a cell or cells of a strain in an in vitro culture.
Claim 9
Independent Claim
49 words
A method as claimed in any one of Claims 1 to 7, wherein said unknown organism being detected and said probe organism are both from the same taxonomic group selected from the group consisting of a kingdom, subkingdom, division, subdivision, phylum, subphylum, class, subclass, order, family, tribe or genus.
Claim 10
Independent Claim
37 words
A method as claimed in any one of Claims 1 to 8, which comprises detecting for the presence of a nucleic acid sequence or sequences creating a taxon below the rank of species or an infrasubspecific subdivision.
Claim 11
Independent Claim
28 words
A method as claimed in any one of Claims 1 to 9, wherein said prokaryotic organism being detected is selectively being detected while present in an eukaryotic organism.
Claim 12
Independent Claim
20 words
A method as claimed in any one of Claims . 1 to 10, wherein said prokaryotic organism is a bacterium.
Claim 13
Independent Claim
29 words
A method as claimed in any one of Claims 1 to 9, wherein said unknown bacterium is present in a fermentation medium or in a secretion or excretion product.
Claim 14
Independent Claim
24 words
A method as claimed in any one of Claims 3 to 9, wherein said unknown bacterium is present in or associated with eukaryotic tissue.
Claim 15
Dependent Claim
20 words
A method as claimed in Claim 13, wherein said bacterium is present in or associated with animal or plant cells.
Claim 16
Dependent Claim
24 words
A method as claimed in Claim 14, wherein said bacterium is present in or associated with human cells, or associated with plant root cells.
Claim 17
Independent Claim
22 words
A method as claimed in any one of Claims 1 to 15, wherein said unknown bacterium is pathogenic towards plants or animals.
Claim 18
Dependent Claim
21 words
A method as claimed in Claim 9, wherein said nucleic acid sequence or sequences are all part of a bacteriophage genome.
Claim 19
Dependent Claim
27 words
A method as claimed in Claim 9, wherein said nucleic acid sequence or sequences are all part of an extrachromosomal genetic element, a plasmid, or an episome.
Claim 20
Dependent Claim
22 words
A method as claimed in Claim 9, wherein said sequence or sequences code for an R-factor or for an antibiotic resistance factor.
Claim 21
Dependent Claim
23 words
A method as claimed in Claim 14, wherein said bacterium is present in or.associated with human cells, or associated with plant root cells.
Claim 22
Independent Claim
22 words
A method as claimed in any one of Claims 1 to 15, wherein said unknown bacterium is pathogenic towards plants or animals.
Claim 23
Dependent Claim
21 words
A method as claimed in Claim 9, wherein said nucleic acid sequence or sequences are all part of a bacteriophage genome..
Claim 24
Dependent Claim
27 words
A method as claimed in Claim 9, wherein said nucleic acid sequence or sequences are all part of an extrachromosomal genetic element, a plasmid, or an ep.isome.
Claim 25
Dependent Claim
22 words
A method as claimed in Claim 9, wherein said sequence or sequences code for an R-factor or for an antibiotic resistance factor.
Claim 26
Independent Claim
30 words
A method of detecting a prokaryotic organism, which method comprises selectively hybridising ribosomal RNA-information-containing nucleic acid of the prokaryotic organism with a complementary detectably labelled prokaryotic ribosomal RNA-information-containing hybridisation probe.
Claim 27
Independent Claim
30 words
A method of detecting a prokaryotic organism, which method comprises selectively hybridising ribosomal RNA-information-containing nucleic acid of the prokaryotic organism with a complementary detectably labelled prokaryotic ribosomal RNA-information-containing hybridisation probe.
References Cited (Backward Citations)
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