Patent EP0168689A2 successfully refreshed from Google Patents.
EP0168689A2
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EP
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Immunoassay for detection of common determinant antibody to hepatitis B
Assignee: Abbott Laboratories
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Filing: 1985-06-28
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Granted: —
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Expires: 2005-06-28
Abstract
Abstract The invention relates to immunoassays for the detection of a common antigenic determinant of hepatitis B virus. More particularly, the invention relates to immunoassays for detecting antibody to hepatitis B surface antigen/a.
Inventors
- 👤 Richard H. Decker
- 👤 Robin A. Gutierrez
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-07 01:34
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/576; G01N33/5761; G01N33/5764
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
105 words
An immunoassay for detecting antibody to hepatitis B surface antigen/a comprising the steps of: a) immobilizing a specific hepatitis B surface antigen/a suotype on a solid phase; b) contacting the solid phase with a test sample; c) contacting the solid phase with another hepatitis B surface antigen/a suotype labeled with a marker, the labeled subtype antigen/a being heterologous to the subtype antigen on tne solid phase; d) separating unreacted reagents from the solid phase; and e) measuring presence of the marker in the solid phase or in 'the unreacted reagents to detect the presence or amount of anti-hepatitis B surface antigen/a present in the sample.
Claim 2
Dependent Claim
105 words
An immunoassay for detecting antibody to hepatitis B surface antigen/a comprising the steps of: a) immobilizing a specific hepatitis B surface antigen/a suotype on a solid phase; b) contacting the solid phase with a test sample; c) contacting the solid phase with another hepatitis B surface antigen/a suotype labeled with a marker, the labeled subtype antigen/a being heterologous to the subtype antigen on tne solid phase; d) separating unreacted reagents from the solid phase; and e) measuring presence of the marker in the solid phase or in 'the unreacted reagents to detect the presence or amount of anti-hepatitis B surface antigen/a present in the sample.
Claim 3
Dependent Claim
11 words
The immunoassay of Claim 1 wherein the marker is an enzyme.
Claim 4
Dependent Claim
11 words
The immunoassay of Claim 1 wherein the marker is a radioisotope.
Claim 5
Dependent Claim
13 words
The immunoassay of Claim 1 wherein steps (b) and (c) are performed simultaneously.
Claim 6
Independent Claim
117 words
A method for detecting antibody to hepatitis B surface antigen subtype/a comprising: a) coating a polystyrene bead with a subtype of hepatitis B surface antigen; b) adding human serum sample to the coated bead; c) incubating for about 2-12 hours at approximately 25-45°C; d) washing the bead with deionized water; e) adding to the bead another subtype of hepatitis B surface antigen labeled with a marker, the labeled subtype antigen being heterologous to the subtype antigen on the bead; f) incubating for about 1-4 hours at approximately 25-45°C; g) washing the bead with deionized water; h) separating unreacted reagents from the bead; and i) measuring presence of the marker in the unreacted reagents or on the bead.
Claim 7
Independent Claim
117 words
A method for detecting antibody to hepatitis B surface antigen subtype/a comprising: a) coating a polystyrene bead with a subtype of hepatitis B surface antigen; b) adding human serum sample to the coated bead; c) incubating for about 2-12 hours at approximately 25-45°C; d) washing the bead with deionized water; e) adding to the bead another subtype of hepatitis B surface antigen labeled with a marker, the labeled subtype antigen being heterologous to the subtype antigen on the bead; f) incubating for about 1-4 hours at approximately 25-45°C; g) washing the bead with deionized water; h) separating unreacted reagents from the bead; and i) measuring presence of the marker in the unreacted reagents or on the bead.
Claim 8
Dependent Claim
32 words
The method of Claim 5 wherein the subtype of hepatitis B surface antigen of step (a) is HBsAg/ay, and the labeled subtype of hepatitis B surface antigen of step (e) is HBsAg/ad.
Claim 9
Dependent Claim
32 words
The method of Claim 5 wherein the subtype of hepatitis B surface antigen of step (a) is HBsAg/ad, and the labeled subtype of hepatitis B surface antigen of step (e) is HBsAg/ay.
Claim 10
Dependent Claim
12 words
The method of Claim 5 wherein the marker is 125 I .
Claim 11
Dependent Claim
11 words
The method of Claim 5 wherein the marker is horseradish peroxidase.
Claim 12
Dependent Claim
25 words
The metnod of Claim 5 wherein step (c) comprises incubating for 4 hours at 40°C, and step (f) comprises incubating for 2 hours at 40°C..
References Cited (Backward Citations)
No citation records indexed.
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