Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
136 words
A circular recombinant DNA compound of formula I wherein the repeated alphabetic sequences (the number of repetitions of which is arbitrarily depicted in said formula) therein represent the following: the "B" region comprises nucleotides whose sequence contains a bGH polyadenylation signal; the "F" region comprises the nucleotides whose ordered codons contain a gene for the synthesis of a pre-selected polypeptide other than bGH, including genomic and other intron containing forms thereof; the "P" region comprises a nucleotide whose sequence contains a promoter capable of initiating transcription of said gene, and the "M" region comprises optional nucleotides whose sequence contains a selectable marker which, upon incorporation of said DNA compound into the expressed genetic material of a living cell, can be utilized to select a cell in which incorporation has taken place; and the acircular forms thereof.
Claim 2
Dependent Claim
136 words
A circular recombinant DNA compound of formula I wherein the repeated alphabetic sequences (the number of repetitions of which is arbitrarily depicted in said formula) therein represent the following: the "B" region comprises nucleotides whose sequence contains a bGH polyadenylation signal; the "F" region comprises the nucleotides whose ordered codons contain a gene for the synthesis of a pre-selected polypeptide other than bGH, including genomic and other intron containing forms thereof; the "P" region comprises a nucleotide whose sequence contains a promoter capable of initiating transcription of said gene, and the "M" region comprises optional nucleotides whose sequence contains a selectable marker which, upon incorporation of said DNA compound into the expressed genetic material of a living cell, can be utilized to select a cell in which incorporation has taken place; and the acircular forms thereof.
Claim 3
Dependent Claim
15 words
A compound according to claim 1 where the "P" region comprises the CMV I.E. promoter.
Claim 4
Dependent Claim
37 words
A compound according to claim 2 wherein the "B" region comprises the bGH gDNA nucleotide sequence from the PstI (Blunt-ended) cleavage site in the codons upstream from the fourth intron downstream to the nearest EcoRI cleavage site.
Claim 5
Dependent Claim
36 words
A compound according to claim 2 wherein the "B" region comprises the bGH gDNA nucleotide sequence from the PvuII cleavage site in the codons downstream from the fourth intron downstream to the nearest EcoRI cleavage site.
Claim 6
Dependent Claim
17 words
A compound according to claim 2 wherein the "B" region comprises the following bGH gDNA nucleotide sequence:
Claim 7
Dependent Claim
15 words
A compound according to claim 5, wherein the "F" region comprises the tPA cDNA gene.
Claim 8
Dependent Claim
22 words
A compound according to claim 1, which has one or more of the characteristics defined in any of claims 2 to 6.
Claim 9
Dependent Claim
20 words
A higher eukaryote cell or cell line whose expressed genetic material comprises a DNA compound according to any preceding claim.
Claim 10
Dependent Claim
15 words
A cell or cell line according to claim 8, wherein the DNA compound expresses tPA.
Claim 11
Dependent Claim
25 words
A method for the expression of a polypeptide, e.g. tPA, which comprises culturing a cell or cell line according to claim 8 or claim 9.