Patent EP0177244A2 successfully refreshed from Google Patents.
EP0177244A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
A method for the detection of peroxidase activity
Assignee: Warner Lambert Co LLC
•
Filing: 1985-09-24
•
Granted: —
•
Expires: 2005-09-24
Abstract
Abstract There is disclosed a method for the detection of peroxidase activity, which method comprises the steps of: a) collecting a specimen which is to be analysed for the presence of peroxidase activity; b) contacting the specimen, with a composition prepared by admixing (i) a tablet comprising from 3% to 40% of an indicator material and from 60% to 97% of an excipient; and (ii) an aqueous-organic reagent solution containing an organic solvent, an oxidizing agent being capable of oxidizing said indicator material in the presence of peroxidase activity, a buffer, and water; and c) detecting the presence or absence of peroxidase activity. A test reagent system and a tablet for use in the method are also disclosed.
Inventors
- 👤 Robert William Schumacher
- 👤 Alexander Mark Schobel
- 👤 Richard George Moran
- 👤 Martin M. Rieger
- 👤 Andrew Robert Gallopo
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-06 20:55
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; C; C12; C12Q; C12Q1/00; C12Q1/26; C12Q1/28; G01N33/72; G01N33/721; G01N33/725; C12Q2326/00
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
101 words
A method for the detection of peroxidase activity, which method comprises the steps of : a) collecting a specimen which is to be analysed for the presence of peroxidase activity; b) contacting the specimen, with a composition prepared by admixing (i) a tablet comprising from 3% to 40 % of an indicator material and from 60% to 97% of an excipient; and (ii) an aqueous-organic reagent solution containing an organic solvent, an oxidizing agent being capable of oxidizing said indicator material in the presence of peroxidase activity, a buffer, and water; and c) detecting the presence or absence of peroxidase activity.
Claim 2
Independent Claim
101 words
A method for the detection of peroxidase activity, which method comprises the steps of : a) collecting a specimen which is to be analysed for the presence of peroxidase activity; b) contacting the specimen, with a composition prepared by admixing (i) a tablet comprising from 3% to 40 % of an indicator material and from 60% to 97% of an excipient; and (ii) an aqueous-organic reagent solution containing an organic solvent, an oxidizing agent being capable of oxidizing said indicator material in the presence of peroxidase activity, a buffer, and water; and c) detecting the presence or absence of peroxidase activity.
Claim 3
Dependent Claim
25 words
A method according to Claim 1, wherein the peroxidase activity to be detected is the presence of hemoglobin in a specimen, for example fecal matter.
Claim 4
Dependent Claim
16 words
A method according to Claim 1 or 2, wherein the indicator material present is gum guaiac.
Claim 5
Dependent Claim
39 words
A method according to Claim 1, 2, 3 or 4, wherein the excipient is chosen from one or more of the following : disintegrating agents, preferably, swelling agents, wicking agents and effervescent agents; lubricating agents; glidants; binders; and diluents.
Claim 6
Independent Claim
27 words
A method according to any preceding claim, wherein the organic solvent is chosen from : alcohols, preferably methanol, ethanol, isopropanol and mixtures thereof; ketones; and mixtures thereof.
Claim 7
Independent Claim
35 words
A method according to any preceding claim, wherein the oxidising agent is chosen from an organic peroxide, or an inorganic peroxide; and is preferably chosen from hydrogen peroxide, urea peroxide, potassium persulphate and mixtures thereof.
Claim 8
Independent Claim
30 words
A method according to any preceding claim, wherein the buffer maintains the pH value of the solution in the range of from 2.0 to 8.0, preferably from 3.5 to 6.5
Claim 9
Independent Claim
23 words
A method according to any preceding claim, wherein the specimen is fecal matter and any peroxidase activity is evidence of fecal occult blood.
Claim 10
Independent Claim
76 words
A method according to any preceding claim, wherein the aqueous-organic reagent solution contains : (a) in the range of from 0.15 to 3.0% by weight of the oxidizing agent; (b) an amount of buffer sufficient to maintain the pH value of the solution in the range of from 2.0 to 8.0; (c) the remainder of an organic solvent capable of solubilizing the indicator material; and (d) water all percentages being by weight of the final composition.
Claim 11
Independent Claim
33 words
A method according to any preceding claim, wherein the aqueous-organic reagent solution contains from 5% to 45% water, at least 55% of the organic solvent, plus the remaining components of the reagent solution.
Claim 12
Dependent Claim
38 words
A tabletted indicator material suitable for use in the detection of occult blood, which tablet comprises: from 3% to 40% gum guaiac and the remainder of an excipient chosen from disintegrating agents, lubricating agents, glidants, binders and diluents.
Claim 13
Dependent Claim
38 words
A tabletted indicator material suitable for use in the detection of occult blood, which tablet comprises: from 3% to 40% gum guaiac and the remainder of an excipient chosen from disintegrating agents, lubricating agents, glidants, binders and diluents.
Claim 14
Dependent Claim
61 words
A test reagent system for detecting the presence of peroxidase activity, which comprises: (a) a tablet containing from 3% to 40% of an indicator material and the remainder of an excipient; and (b) an aqueous-organic reagent solution containing water, an organic solvent, an oxidizing agent being capable of oxidizing said indicator material in the presence of peroxidase activity, and a buffer.
Claim 15
Dependent Claim
61 words
A test reagent system for detecting the presence of peroxidase activity, which comprises: (a) a tablet containing from 3% to 40% of an indicator material and the remainder of an excipient; and (b) an aqueous-organic reagent solution containing water, an organic solvent, an oxidizing agent being capable of oxidizing said indicator material in the presence of peroxidase activity, and a buffer.
Claim 16
Dependent Claim
33 words
A test reagent system according to Claim 12, wherein the oxidizing agent is chosen from an organic peroxide, or an inorganic peroxide, preferably from hydrogen peroxide, urea peroxide, potassium persulphate and mixtures thereof.
Claim 17
Dependent Claim
31 words
A test reagent system according to Claim 12 or 13, wherein the buffer maintains the pH of the solution in the range of from 2.0 to 8.0, preferably 3.5 to 6.5.
Claim 18
Dependent Claim
30 words
A test reagent according to Claim 12, 13 or 14, wherein the organic solvent is chosen from alcohols, ketones, and mixtures thereof, preferably from methanol, ethanol, isopropanol and mixtures thereof.
Claim 19
Dependent Claim
81 words
A test reagent system according to Claim 12, 13, 14 or 15, wherein the aqueous-organic reagent solution contains : (a) in the range of from 0.15 to 3.0% by weight of the oxidizing agent; (b) an amount of buffer sufficient to maintain the pH value of the liquid solution in the range of 2.0 to 8.0; (c) the remainder of an organic solvent capable of solubilizing the indicator material; and (d) water; all percentages being by weight of the final composition.
Claim 20
Dependent Claim
38 words
A test reagent system according to any one of Claims 12 to 16, the aqueous-organic reagent solution contains from 5% to 45% water, at least 55% of the organic solvent, plus the remaining components of the reagent solution.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
Maintenance schedule is calculated for granted US utility patents.