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EP0185404A1
Expired / Lapsed
EP
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Stroma-free hemoglobin solution, process for the preparation thereof and its application
Assignee: EURO-TROL B.V.
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Filing: 1985-11-08
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Granted: —
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Expires: 2005-11-08
Abstract
Abstract Hemoglobin solution is stabilized by adding inositol hexaphosphate to give a molar ratio IHP/HB of from 0.1:1 to 1:1 by preference reduced NAD is also added. The stabilized solution is l.A. suitable for calibrating blood-gas control apparatus.
Inventors
- 👤 Ronald Sprokholt
- 👤 Antonius Henricus Josephus Maas
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-04 03:29
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/96; C; C07; C07K; C07K14/00; C07K14/795; C07K14/805; A; A61; A61M; A61M1/00; A61M1/36; A61M1/3693; A61M2202/00; A61M2202/04; A61M2202/0413; A61M2202/0429; A61M2202/0433; G01N2333/00; G01N2333/795; G01N2333/805; G01N2496/00; G01N2496/70; G01N2496/80; G01N33/72
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
24 words
STROMA-FREE HEMOGLOBIN SOLUTION, CHARACTERIZED IN THAT IT CONTAINS INOSITOL HEXAPHOSPHATE, IN SUCH AN AMOUNT THAT THE MOLAR RATIO IHP/HB IS FROM 0.1:1 TO 1.0:1.
Claim 2
Dependent Claim
24 words
STROMA-FREE HEMOGLOBIN SOLUTION, CHARACTERIZED IN THAT IT CONTAINS INOSITOL HEXAPHOSPHATE, IN SUCH AN AMOUNT THAT THE MOLAR RATIO IHP/HB IS FROM 0.1:1 TO 1.0:1.
Claim 3
Dependent Claim
18 words
STOMA-FREE HEMOGLOBIN-SOLUTION ACCORDING TO CLAIM 1 CHARACTERIZED IN THAT IT ALSO CONTAINS METHEMOGLOBIN REDUCTASE AND REDUCED NAD (NADH).
Claim 4
Dependent Claim
15 words
STROMA-FREE HEMOGLOBIN SOLUTION ACCORDING TO CLAIM 2, CHARACTERIZED IN THAT IT ALSO CONTAINS CYCTOCHROME B5.
Claim 5
Dependent Claim
28 words
STROMA-FREE HEMOGLOBIN SOLUTION ACCORDING TO ANY OF THE PRECEDING CLAIMS, CHARACTERIZED IN THAT IT CONTAINS FROM 3 TO 15 MMOL/LHB AND HAS A PH FROM 6,9 TO 8,1.
Claim 6
Dependent Claim
27 words
STROMA-FREE HEMOGLOBIN SOLUTION ACCORDING TO ANY OF THE PRECEDING CLAIMS, CHARACTERIZED IN THAT AFTER BEING KEPT FOR 1 YEAR AT BELOW 4°C IT STILL CONTAINS NO PRECIPITATE.
Claim 7
Dependent Claim
14 words
STROMA-FREE HEMOGLOBIN COMPOSITION ACCORDING TO ANY OF THE PRECEDING CLAIMS CONSISTING OF THREE SOLUTIONS,
Claim 8
Dependent Claim
11 words
WITH A PH OF ABOUT 7.2, ABOUT 7.4 AND ABOUT 7.6.
Claim 9
Dependent Claim
11 words
WITH A PH OF ABOUT 7.2, ABOUT 7.4 AND ABOUT 7.6.
Claim 10
Dependent Claim
85 words
PROCESS FOR PREPARING A STROMA-FREE HEMOGLOBIN-SOLUTION, WHEREIN PREVIOUSLY PURIFIED ERYTHOCYTES ARE LYSED BY ADDING DISTILLED OR DEMINERALIZED WATER AND IN THE LYSATE THE CONTENTS OF CA; NA; K; HCO 3 AND CL' ARE ADJUSTED AT THE VALUES CORRESPONDING WITH THOSE OF BLOOD PLASMA, BY GASSING THE PCO 2 AND PO 2 ARE ADJUSTED AT THE DESIRED VALUES, AND THE FINAL COMPOSITION IS STERILIZED BY FILTERING, CHARACTERIZED IN THAT MOREOVER SO MUCH INOSITOL HEXAPHOSPHATE IS ADDED THAT THE MOLAR RATIO IHP/HB IS FROM 0.1:1 TO 1.0:1.
Claim 11
Dependent Claim
85 words
PROCESS FOR PREPARING A STROMA-FREE HEMOGLOBIN-SOLUTION, WHEREIN PREVIOUSLY PURIFIED ERYTHOCYTES ARE LYSED BY ADDING DISTILLED OR DEMINERALIZED WATER AND IN THE LYSATE THE CONTENTS OF CA; NA; K; HCO 3 AND CL' ARE ADJUSTED AT THE VALUES CORRESPONDING WITH THOSE OF BLOOD PLASMA, BY GASSING THE PCO 2 AND PO 2 ARE ADJUSTED AT THE DESIRED VALUES, AND THE FINAL COMPOSITION IS STERILIZED BY FILTERING, CHARACTERIZED IN THAT MOREOVER SO MUCH INOSITOL HEXAPHOSPHATE IS ADDED THAT THE MOLAR RATIO IHP/HB IS FROM 0.1:1 TO 1.0:1.
Claim 12
Dependent Claim
88 words
PROCESS ACCORDING TO CLAIM 7, CHARACTERIZED IN THAT TO THE LYSATE SO MUCH IHP IS ADDED, THAT THE MOLAR RATIO IHP/HB IS ABOUT 0.5:1, THE PH IS ADJUSTED BETWEEN 8.0 AND 8.1 WITH DILUTED NAOH, THE PRECIPITATE IS REMOVED BY CENTRIFUGING. THE PH ADJUSTED BETWEEN 6.9 AND 7.0 WITH DILUTED HCL, THE PRECIPITATE IS REMOVED BY CENTRIFUGING, AND FINALLY AFTER ADDITION OF CA; K', NA', HCO 3 AND CL' TO ATTAIN THE DESIRED CONTENTS, THE PH AND PCO 2 AND P0 2 ARE ADJUSTED AT THE DESIRED VALUES.
Claim 13
Dependent Claim
30 words
PROCESS FOR DETERMINING PH, PCO 2 ' P0 2 AND/OR HB IN BLOOD, CHARACTERIZED IN THAT SAID DETERMINATION IS CALIBRATED WITH A SOLUTION OBTAINED ACCORDING TO ANY OF CLAIMS 1-6.
Claim 14
Dependent Claim
13 words
APPLICATION OF A COMPOSITION ACCORDING TO ANY OF CLAIMS 1-6 AS BLOOD SUBSTITUTE.
References Cited (Backward Citations)
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