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EP0210461A2 Expired / Lapsed EP ⏱️ 0.0 years remaining

Physiologically active polypeptide BUF-3

Assignee: Ajinomoto Co Inc
•
Filing: 1986-07-02
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Granted: —
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Expires: 2006-07-02
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Abstract

Abstract Polypeptide capable of differentiating and maturating mouse leukemia cells into normal cells, having the following properties : (a) Molecular weight 16±1 Kd ( SDS-electrophoresis in the presence of 1.0% mercaptoethanol ) 25±1 Kd ( SDS-electrophoresis in the absence of mercaptoethanol ) (b) Amino acid sequence at amino terminal Gly-Leu-Glu-X-Asp-Gly-Lys-Val-Asn-Ile-X-X-Lys-Lys-­Gln-Phe-Phe-Val-Ser-Phe-Lys-Asp-Ile-Gly-Trp-Asn-Asp-­Trp-Ile-Ile-Ala-Pro-Ser-Gly-Tyr ( X: unidentified amino acid ) (c) Amino acid sequence of peptide fragment formed by cyanogen bromide cleavage Leu-Tyr-Tyr-Asp-Asp-Gly-Gln-Asn-Ile-Ile-Lys-Lys-Asp-­Ile-Gln-Asn

Inventors

  • 👤 Yuzuru Eto
  • 👤 Tomoko Tsuji
  • 👤 Satoshi Takano
  • 👤 Misako Takezawa
  • 👤 Yasunori Yokogawa
  • 👤 Hiroshiro Shibai

Bibliographic Details

Application Number —
Jurisdiction EP
Data Source google_patents
Last Synchronized 2026-10-23 22:45
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/575; A; A61; A61P; A61P35/00; A61P35/02; A61P7/00; A61P7/06; A61K; A61K38/00; Y; Y10; Y10S; Y10S930/00; Y10S930/01; Y10S930/26

Claims Analysis

Total Claims: 5
Claim 1
Independent Claim 69 words
A polypeptide capable of differentiating and maturating mouse leukemia cells into normal cells and having the following properties: (a) Molecular weight 16±1 Kd ( SDS-electrophoresis in the presence of 1.0% mercaptoethanol ) 25±1 Kd ( SDS-electrophoresis in the absence of mercaptoethanol ) (b) Amino acid sequence at amino terminal Gly-Leu-Glu-X-Asp-Gly-Lys-Val-Asn-Ile-X-X-Lys-Lys-­Gln-Phe-Phe-Val-Ser-Phe-Lys-Asp-Ile-Gly-Trp-Asn-Asp-­Trp-Ile-Ile-Ala-Pro-Ser-Gly-Tyr ( X: unidentified amino acid ) (c) Amino acid sequence of peptide fragment formed by cyanogen bromide cleavage Leu-Tyr-Tyr-Asp-Asp-Gly-Gln-Asn-Ile-Ile-Lys-Lys-Asp-­Ile-Gln-Asn
Claim 2
Dependent Claim 69 words
A polypeptide capable of differentiating and maturating mouse leukemia cells into normal cells and having the following properties: (a) Molecular weight 16±1 Kd ( SDS-electrophoresis in the presence of 1.0% mercaptoethanol ) 25±1 Kd ( SDS-electrophoresis in the absence of mercaptoethanol ) (b) Amino acid sequence at amino terminal Gly-Leu-Glu-X-Asp-Gly-Lys-Val-Asn-Ile-X-X-Lys-Lys-­Gln-Phe-Phe-Val-Ser-Phe-Lys-Asp-Ile-Gly-Trp-Asn-Asp-­Trp-Ile-Ile-Ala-Pro-Ser-Gly-Tyr ( X: unidentified amino acid ) (c) Amino acid sequence of peptide fragment formed by cyanogen bromide cleavage Leu-Tyr-Tyr-Asp-Asp-Gly-Gln-Asn-Ile-Ile-Lys-Lys-Asp-­Ile-Gln-Asn
Claim 3
Dependent Claim 38 words
A polypeptide capable of differentiating and maturating mouse leukemia cells into normal cells, obtainable by culturing human leukemia cells in the presence of at least one differen­tiation inducing substance and isolating the accumulated poly­peptide from the culture medium.
Claim 4
Dependent Claim 38 words
A polypeptide capable of differentiating and maturating mouse leukemia cells into normal cells, obtainable by culturing human leukemia cells in the presence of at least one differen­tiation inducing substance and isolating the accumulated poly­peptide from the culture medium.
Claim 5
Dependent Claim 22 words
A polypeptide according to claim 2, wherein the differentiation inducing substance is Actinomycin D, Mito­mycin C, concanavalin A or a phorbol ester.

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