Patent EP0230786A1 successfully refreshed from Google Patents.
EP0230786A1
Expired / Lapsed
EP
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Assay for cholesterol and derivatives thereof
Assignee: Medisense Inc
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Filing: 1986-12-24
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Granted: —
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Expires: 2006-12-24
Abstract
Abstract A cholesterol sensor is a dry strip sensor which comprises an elongate substrate (l) with a screen printed reference electrode (6) and a screen printed working electrode (5), the working electrode comprising a conducting surface carrying optional cholesterol esterase, a cholesterol oxidase, a peroxidase, and a mediator which transfers charge between the conducting surface and the peroxidase when the peroxide is catalytically active.
Inventors
- 👤 Hugh Allen Oliver Hill
- 👤 Jane Elizabeth Frew
- 👤 Michael Richard Ball
- 👤 Monika Joanna Green
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-07 20:29
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/60; C12Q1/001; C12Q1/004
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
73 words
A sensor for use in the assay of total cholesterol in blood, the sensor being a dry strip sensor comprising an elongate substrate with a screen printed reference electrode and a screen printed working electrode, the working electrode comprising a conducting surface carrying a surfactant, a cholesterol esterase, a cholesterol oxidase, a peroxidase, and a mediator which transfers electrons between the conducting surface and the peroxidase in the presence of peroxide as substrate.
Claim 2
Dependent Claim
73 words
A sensor for use in the assay of total cholesterol in blood, the sensor being a dry strip sensor comprising an elongate substrate with a screen printed reference electrode and a screen printed working electrode, the working electrode comprising a conducting surface carrying a surfactant, a cholesterol esterase, a cholesterol oxidase, a peroxidase, and a mediator which transfers electrons between the conducting surface and the peroxidase in the presence of peroxide as substrate.
Claim 3
Dependent Claim
69 words
The sensor of claim l, which comprises an elongate, electrically-insulating substrate having a pair of longitudinal, substantially parallel, electrically-conducting tracks thereupon, each track being provided at the same end thereof with means for electrical connection with read-out means and provided at the other end thereof with an electrode, one of the electrodes being the reference electrode and the other being the working electrode responsive to the presence of cholesterol.
Claim 4
Dependent Claim
70 words
A sensor for use in the assay of free cholesterol in blood, the sensor being a dry strip sensor comprising an elongate substrate with a screen printed reference electrode and a screen printed working electrode, the working electrode comprising a conducting surface carrying a surfactant, a cholesterol oxidase, a peroxidase, and a mediator which transfers charge between the conducting surface and the peroxidase in the presence of peroxide as substrate
Claim 5
Dependent Claim
70 words
A sensor for use in the assay of free cholesterol in blood, the sensor being a dry strip sensor comprising an elongate substrate with a screen printed reference electrode and a screen printed working electrode, the working electrode comprising a conducting surface carrying a surfactant, a cholesterol oxidase, a peroxidase, and a mediator which transfers charge between the conducting surface and the peroxidase in the presence of peroxide as substrate
Claim 6
Dependent Claim
69 words
The sensor of claim 3, which comprises an elongate, electrically-insulating substrate having a pair of longitudinal, substantially parallel, electrically-conducting tracks thereupon, each track being provided at the same end thereof with means for electrical connection with read-out means and provided at the other end thereof with an electrode, one of the electrodes being the reference electrode and the other being the working electrode responsive to the presence of cholesterol.
Claim 7
Dependent Claim
19 words
An assay for total cholesterol, wherein the cholesterol is amperometrically quantitated using a sensor as defined in claim l.
Claim 8
Dependent Claim
19 words
An assay for free cholesterol, wherein the cholesterol is amperometrically quantitated using a sensor as defined in claim 3.
References Cited (Backward Citations)
EP0121385A1
Cited
EP0125867A2
Cited
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