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EP0330120A2 Expired / Lapsed EP ⏱️ 0.0 years remaining

Fluorescence detection type electrophoresis apparatus

Assignee: Hitachi Ltd
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Filing: 1989-02-20
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Granted: —
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Expires: 2009-02-20
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Abstract

Abstract In an electrophoresis apparatus for separating and detecting a sample (32) of DNA or RNA labelled with fluorescence, use is made of a gel plate (4) having a polyacrylamide concentration of 2 to about 6% in order to greatly shorten the time required for the measurement.

Inventors

  • 👤 Hideki Kambara
  • 👤 Yoshiko Katayama
  • 👤 Tetsuo Nishikawa

Bibliographic Details

Application Number —
Jurisdiction EP
Data Source google_patents
Last Synchronized 2026-10-25 16:17
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44704; G01N27/44717; G01N27/44721

Claims Analysis

Total Claims: 6
Claim 1
Independent Claim 73 words
A fluorescence detection type electrophoresis apparatus for determining base sequence of a sample, comprising an electrophoresis separation device (30) for electrophoresis-separating said sample (32) labelled with fluorescence, an excitation light source (31) for exciting said sample, and a detection means (33) for detecting the fluorescence emitted by said sample that is excited, wherein a gel plate (4) having a polyacrylamide concentration of 2 to about 6% is used as said electro­phoresis separation device.
Claim 2
Dependent Claim 73 words
A fluorescence detection type electrophoresis apparatus for determining base sequence of a sample, comprising an electrophoresis separation device (30) for electrophoresis-separating said sample (32) labelled with fluorescence, an excitation light source (31) for exciting said sample, and a detection means (33) for detecting the fluorescence emitted by said sample that is excited, wherein a gel plate (4) having a polyacrylamide concentration of 2 to about 6% is used as said electro­phoresis separation device.
Claim 3
Dependent Claim 18 words
A fluorescence detection type electrophoresis apparatus according to claim 1, wherein said sample (32) is DNA or RNA.
Claim 4
Dependent Claim 21 words
A fluorescence detection type electrophoresis apparatus according to claim 1 or 2, wherein said polyacrylamide concentration is from 4.3 to 6.2%.
Claim 5
Dependent Claim 21 words
A fluorescence detection type electrophoresis apparatus according to claim 1 or 2, wherein said polyacrylamide concentration is from 3.2 to 4.3%.
Claim 6
Dependent Claim 21 words
A fluorescence detection type electrophoresis apparatus according to claim 1 or 2, wherein said polyacrylamide concentration is from 2.6 to 3.2%.

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