Patent EP0333523A2 successfully refreshed from Google Patents.
EP0333523A2
Expired / Lapsed
EP
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Method of potentiating an immune response and compositions therefor
Assignee: UAB Research Foundation
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Filing: 1989-03-20
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Granted: —
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Expires: 2009-03-20
Abstract
Abstract A method, and compositions for use therein capable, of delivering a bioactive agent to an animal entailing the steps of encapsulating effective amounts of the agent in a biocompatible excipient to form microcapsules having a size less than approximately ten micrometers and administering effective amounts of the microcapsules to the animal. A pulsatile response is obtained, as well as mucosal and systemic immunity.
Inventors
- 👤 Thomas T. Tice
- 👤 John H. Eldridge
- 👤 Richard M. Gilley
- 👤 Jay K Stass
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-30 10:32
CPC Classifications
A; A61; A61K; A61K31/00; A61K31/21; A61K31/215; A61K31/216; A61K9/00; A61K9/48; A61K9/50; A61K39/00; A61K39/02; A61K39/085; A61K39/12; A61K39/39; A61K9/14; A61K9/16; A61K9/1605; A61K9/1629; A61K9/1635; A61K9/1641; A61K9/1647; A61P; A61P37/00; A61P37/02; A61P37/04; A61K2039/54; A61K2039/541; A61K2039/545; A61K2039/555; A61K2039/55511; A61K2039/55555; A61K38/00; C; C12; C12N; C12N2770/00; C12N2770/00011; C12N2770/24011; C12N2770/24111; C12N2770/24134; Y; Y02; Y02A; Y02A50/00; Y02A50/30; Y10; Y10T; Y10T428/00; Y10T428/29; Y10T428/2982; Y10T428/2984; Y10T428/2985; Y10T428/2989
Claims Analysis
Total Claims: 69
Claim 1
Independent Claim
41 words
A composition for delivering a bioactive agent to the mucosally associated lymphoreticular tissues of an animal, the composition comprising an effective amount of said agent encapsulated in a biocompatible excipient to form microcapsules having a size less than approximately 10 micrometers.
Claim 2
Dependent Claim
41 words
A composition for delivering a bioactive agent to the mucosally associated lymphoreticular tissues of an animal, the composition comprising an effective amount of said agent encapsulated in a biocompatible excipient to form microcapsules having a size less than approximately 10 micrometers.
Claim 3
Dependent Claim
19 words
The composition of Claim 1 wherein said microcapsules have a size between approximately 1 micrometer and approximately 10 micrometers.
Claim 4
Dependent Claim
28 words
The composition of Claim 1 or Claim 2 wherein said agent is selected from the group consisting of a drug, nutrient, immunomodulator, lymphokine, monokine, cytokine, antigen and allergen.
Claim 5
Dependent Claim
39 words
The composition of any one of Claims 1 to 3 wherein said microcapsules have a size ranging from between approximately 5 micrometers and approximately 10 micrometers so that said microcapsules can be retained in said mucosally asociated lymphoreticular tissues.
Claim 6
Dependent Claim
33 words
The composition of any one of Claims 1 to 3 wherein said microcapsules have a size less then approximately 5 micrometers so that said microcapsules can pass through said mucosally associated lymphoreticular tissues.
Claim 7
Dependent Claim
19 words
The composition of Claim 5 wherein said microcapsules have a size from approximately 1 micrometer and approximately 5 micrometers.
Claim 8
Dependent Claim
55 words
The composition of Claim 3 wherein said microcapsules comprise a mixture of a plurality of first microcapsules having a size less than approximately 5 micrometers and a plurality of second microcapsules having a size between approximately 5 micrometers and approximately 10 micrometers for providing both a systemic immunity and a mucosal immunity to said animal.
Claim 9
Dependent Claim
20 words
The composition of Claim 7 wherein said first microcapsules have a size between approximately 1 micrometer and approximately 5 micrometers.
Claim 10
Dependent Claim
40 words
A composition for providing systemic immunity in an animal, the composition comprising an effective amount of a bioactive agent encapsulated in a biocompatible excipient to form microcapsules having a size between approximately 1 micrometer and approximately 10 micrometers in diameter.
Claim 11
Dependent Claim
40 words
A composition for providing systemic immunity in an animal, the composition comprising an effective amount of a bioactive agent encapsulated in a biocompatible excipient to form microcapsules having a size between approximately 1 micrometer and approximately 10 micrometers in diameter.
Claim 12
Dependent Claim
23 words
The composition of Claim 9 wherein said bioactive agent is selected from the group consisting of an antigen, allergen, lymphokine, monokine and immunomodulator.
Claim 13
Dependent Claim
77 words
A composition for potentiating the immune response of an animal, the composition comprising a mixture of effective amounts of first biocompatible microcapsules having a size less than approximately 10 micrometers and containing a first bioactive agent and second biocompatible microcapsules having a size greater than approximately 10 micrometers and containing a second bioactive agent, said first microcapsules providing a primary immunological response and said second microcapsules releasing said second agent pulsatily to potentiate a subsequent immunological response.
Claim 14
Dependent Claim
77 words
A composition for potentiating the immune response of an animal, the composition comprising a mixture of effective amounts of first biocompatible microcapsules having a size less than approximately 10 micrometers and containing a first bioactive agent and second biocompatible microcapsules having a size greater than approximately 10 micrometers and containing a second bioactive agent, said first microcapsules providing a primary immunological response and said second microcapsules releasing said second agent pulsatily to potentiate a subsequent immunological response.
Claim 15
Dependent Claim
28 words
The composition of Claim 11 wherein at least one of said bioactive agents is selected from the group consisting of an antigen, allergen, lymphokine, monokine, cytokine and immunomodulator.
Claim 16
Dependent Claim
32 words
A composition for increasing the bioavailability of a bioactive agent through oral administration and comprising biocompatible microcapsules containing effective amounts of said agent and having a size less than approximately 10 micrometers.
Claim 17
Dependent Claim
32 words
A composition for increasing the bioavailability of a bioactive agent through oral administration and comprising biocompatible microcapsules containing effective amounts of said agent and having a size less than approximately 10 micrometers.
Claim 18
Dependent Claim
25 words
The composition of Claim 13 wherein said agent is selected from the group consisting of a drug, immunomodulator, lymphokine, monokine, cytokine, nutrient, antigen and allergen.
Claim 19
Dependent Claim
47 words
A composition for potentiating the immune response of an animal, the composition comprising a mixture of a first, free bioactive agent to provide a primary response and microcapsules having a biocompatible excipient wall and containing a second bioactive agent for release pulsatily to potentiate a subsequent response.
Claim 20
Dependent Claim
47 words
A composition for potentiating the immune response of an animal, the composition comprising a mixture of a first, free bioactive agent to provide a primary response and microcapsules having a biocompatible excipient wall and containing a second bioactive agent for release pulsatily to potentiate a subsequent response.
Claim 21
Dependent Claim
24 words
The composition of Claim 15 wherein said first and second agents are an antigen or wherein said first and second agents are an allergen.
Claim 22
Dependent Claim
25 words
The composition of Claim 15 wherein at least one said agents is an antigen, an allergen, a lymphokine, a cytokine, a monokine, or an immunomodulator.
Claim 23
Dependent Claim
21 words
The composition of any one of Claims 15 to 17 wherein said microcapsules have a size greater than approximately 1 micrometer.
Claim 24
Dependent Claim
24 words
The composition of any one of Claims 11 to 17 wherein said microcapsules have a size between approximately 1 micrometer and approximately 10 micrometers.
Claim 25
Dependent Claim
21 words
The composition of any one of Claims 15 to 17 wherein said microcapsules have a size greater than approximately 10 micrometers.
Claim 26
Independent Claim
73 words
A method of delivering a bioactive agent to the mucosally associated lymphoreticular tissues of an animal, comprising the steps of:- (a) encapsulating effective amounts of said agent in a biocompatible excipient to form microcapsules having a size less than approximately 10 micrometers, and (b) administering an effective amount of said microcapsules to said animals so that a therapeutic amount of said microcapsules reach and are taken up by said mucosally associated lymphoreticular tissues.
Claim 27
Independent Claim
73 words
A method of delivering a bioactive agent to the mucosally associated lymphoreticular tissues of an animal, comprising the steps of:- (a) encapsulating effective amounts of said agent in a biocompatible excipient to form microcapsules having a size less than approximately 10 micrometers, and (b) administering an effective amount of said microcapsules to said animals so that a therapeutic amount of said microcapsules reach and are taken up by said mucosally associated lymphoreticular tissues.
Claim 28
Dependent Claim
18 words
The method of Claim 21 wherein said administering step is selected from oral, nasal, rectal and ophthalmic administration.
Claim 29
Dependent Claim
13 words
The method of Claim 21, wherein said administering step is by oral inhalation.
Claim 30
Independent Claim
30 words
The method of any one of Claims 21 to 23 wherein said agent is selected from the group consisting of a drug, nutrient, immunomodulator, lymphokine, monokine, cytokine, antigen and allergen.
Claim 31
Independent Claim
24 words
The method of any one of Claims 21 to 24 wherein said microcapsules have a size between approximately 1 micrometer and approximately 10 micrometers.
Claim 32
Dependent Claim
33 words
The method of Claim 25 wherein said microcapsules have a size ranging from between approximately 5 micrometers and approximately 10 micrometers so that microcapsules can be retained in said mucosally associated lymphoreticular tissues.
Claim 33
Independent Claim
33 words
The method of any one of Claims 21 to 24 wherein said microcapsules have a size less than approximately 5 micrometers so that said microcapsules can pass through said mucosally associated lymphoreticular tissues.
Claim 34
Dependent Claim
31 words
The method of Claim 25 wherein said microcapsules have a size between approximately 1 micrometer and approximately 5 micrometers so that said microcapsules can pass through said mucosally associated lymphorecticular tissues.
Claim 35
Dependent Claim
31 words
The method of Claim 26 wherein said bioactive agent is an antigen to provide a mucosal immunity for said animal or an allergen to provide a mucosal immunity for said animal.
Claim 36
Dependent Claim
31 words
The method of Claim 27 wherein said bioactive agent is an antigen to provide a systemic immunity for said animal or an allergen to provide a systemic immunity for said animal.
Claim 37
Dependent Claim
69 words
The method of Claim 24 wherein said microcapsules comprise a plurality of first microcapsules having a size less than approximately 5 micrometers and a plurality of second microcapsules having a size between approximately 5 micrometers and approximately 10 micrometers, and wherein said administering step comprises the delivery of a mixture of said first and second microcapsules to said animal to provide both a systemic immunity and a mucosal immunity.
Claim 38
Dependent Claim
20 words
The method of Claim 31 wherein said first microcapsules have a size between approximately 1 micrometer and approximately 5 micrometers.
Claim 39
Dependent Claim
29 words
The method of Claim 31 wherein said microcapsules have a size of less than approximately 5 micrometers so that said microcapsules can pass through said mucosally associated lymphoreticular tissues.
Claim 40
Dependent Claim
19 words
The method of Claim 33 wherein said microcapsules have a size between approximately 1 micrometer and approximately 5 micrometers.
Claim 41
Independent Claim
80 words
A method of immunizing an animal, the method comprising the step of administering to said animal a mixture of an effective amount of a first free bioactive agent and microcapsules having a biocompatible excipient wall material and containing a second bioactive agent, wherein said microcapsules are of a size greater than approximately 10 micrometers and wherein said first bioactive agent provides a primary immunological response and said microcapsules release said second bioactive agent pulsatily to potentiate a subsequent immunological response.
Claim 42
Independent Claim
80 words
A method of immunizing an animal, the method comprising the step of administering to said animal a mixture of an effective amount of a first free bioactive agent and microcapsules having a biocompatible excipient wall material and containing a second bioactive agent, wherein said microcapsules are of a size greater than approximately 10 micrometers and wherein said first bioactive agent provides a primary immunological response and said microcapsules release said second bioactive agent pulsatily to potentiate a subsequent immunological response.
Claim 43
Independent Claim
84 words
A method of potentiating the immune response of an animal, the method comprising the step of administering to said animal a mixture of effective amounts of first biocompatible microcapsules having a size less than approximately 10 micrometers and containing a first bioactive agent and second biocompatible microcapsules having a size greater than approximately 10 micrometers and containing a second bioactive agent, said first microcapsules providing a primary immunological response and said second microcapsules releasing said second agent pulsatily to potentiate a subsequent immunological response.
Claim 44
Independent Claim
84 words
A method of potentiating the immune response of an animal, the method comprising the step of administering to said animal a mixture of effective amounts of first biocompatible microcapsules having a size less than approximately 10 micrometers and containing a first bioactive agent and second biocompatible microcapsules having a size greater than approximately 10 micrometers and containing a second bioactive agent, said first microcapsules providing a primary immunological response and said second microcapsules releasing said second agent pulsatily to potentiate a subsequent immunological response.
Claim 45
Dependent Claim
20 words
The method of Claim 36 wherein said first microcapsules have a size between approximately 1 micrometer and approximately 10 micrometers.
Claim 46
Independent Claim
33 words
The method of any one of Claims 35 to 37 wherein at least one of said bioactive agents is selected from the group consisting of an antigen, allergen, lymphokine, monokine, cytokine and immunomodulator.
Claim 47
Independent Claim
53 words
A method of providing systemic immunity in an animal, the method comprising the steps of:- (a) encapsulating effective amounts of an antigen in a biocompatible excipient to form microcapsules having a size less than approximately 5 micrometers, and (b) administering said microcapsules to said animal orally, nasally, by oral inhalation, rectally or ophthalmically.
Claim 48
Independent Claim
53 words
A method of providing systemic immunity in an animal, the method comprising the steps of:- (a) encapsulating effective amounts of an antigen in a biocompatible excipient to form microcapsules having a size less than approximately 5 micrometers, and (b) administering said microcapsules to said animal orally, nasally, by oral inhalation, rectally or ophthalmically.
Claim 49
Dependent Claim
21 words
A method as claimed in Claim 39 wherein said microcapsules have a size between approximately 1 micrometer and approximately 5 micrometers.
Claim 50
Independent Claim
57 words
A method of providing mucosal immunity in an animal, the method comprising the steps of: (a) encapsulating effective amounts of an antigen in a biocompatible excipient to form microcapsules having a size from between approximately 5 micrometers and approximately 10 micrometers, and (b) administering said microcapsules to said animal orally, nasally, by oral inhalation, rectally or ophthalmically.
Claim 51
Independent Claim
57 words
A method of providing mucosal immunity in an animal, the method comprising the steps of: (a) encapsulating effective amounts of an antigen in a biocompatible excipient to form microcapsules having a size from between approximately 5 micrometers and approximately 10 micrometers, and (b) administering said microcapsules to said animal orally, nasally, by oral inhalation, rectally or ophthalmically.
Claim 52
Independent Claim
83 words
A method of providing systemic and mucosal immunity to an animal, the method comprising administering to said animal a plurality of first microcapsules containing antigen and having a size less than approximately 5 micrometers and a plurality of second microcapsules containing antigen and having a size between approximately 5 micrometers and approximately 10 micrometers, and wherein said administering step comprises the delivery of a mixture of said first and second microcapsules to said animal to provide both a systemic immunity and mucosal immunity.
Claim 53
Independent Claim
83 words
A method of providing systemic and mucosal immunity to an animal, the method comprising administering to said animal a plurality of first microcapsules containing antigen and having a size less than approximately 5 micrometers and a plurality of second microcapsules containing antigen and having a size between approximately 5 micrometers and approximately 10 micrometers, and wherein said administering step comprises the delivery of a mixture of said first and second microcapsules to said animal to provide both a systemic immunity and mucosal immunity.
Claim 54
Dependent Claim
27 words
The method of Claim 42 wherein said administering step is selected from the group consisting of oral administration, nasal administration, oral inhalation, rectal administration, and ophthalmic administration.
Claim 55
Dependent Claim
22 words
The method of Claims 42 or Claim 43 wherein said first microcapsules have a size between approximately 1 micrometer and 5 micrometers.
Claim 56
Independent Claim
47 words
A method of providing a composition for delivering a bioactive agent to the mucosally associated lymphoreticular tissues of an animal, the method comprising the steps of encapsulating effective amounts of said agent in a biocompatible excipient to form microcapsules having a size less than approximately 10 micrometers.
Claim 57
Independent Claim
47 words
A method of providing a composition for delivering a bioactive agent to the mucosally associated lymphoreticular tissues of an animal, the method comprising the steps of encapsulating effective amounts of said agent in a biocompatible excipient to form microcapsules having a size less than approximately 10 micrometers.
Claim 58
Dependent Claim
60 words
The method of Claim 45 wherein said microcapsules are comprised of a plurality of first microcapsules having a size less than approximately 5 micrometers and a plurality of second microcapsules having a size between approximately 5 micrometers and approximately 10 micrometers, said first and second microcapsules being administered to said animal to provide both a systemic and a mucosal immunity.
Claim 59
Independent Claim
75 words
A method of preparing a composition for potentiating the immune response of an animal, the method comprising the step of adding together effective amounts of a first, fee bioactive agent and microcapsules having a biocompatible excipient wall and containing a second bioactive agent to form a mixture which is administered to an animal wherein said first agent provides a primary response and wherein said microcapsules release said second agent pulsatily to potentiate a subsequent response.
Claim 60
Independent Claim
75 words
A method of preparing a composition for potentiating the immune response of an animal, the method comprising the step of adding together effective amounts of a first, fee bioactive agent and microcapsules having a biocompatible excipient wall and containing a second bioactive agent to form a mixture which is administered to an animal wherein said first agent provides a primary response and wherein said microcapsules release said second agent pulsatily to potentiate a subsequent response.
Claim 61
Dependent Claim
19 words
The method of Claim 47 wherein at least one of said bioactive agents is an antigen or an allergen.
Claim 62
Dependent Claim
19 words
The method of Claim 47 or Claim 48 wherein said microcapsules have a size greater than approximately 10 micrometers.
Claim 63
Independent Claim
50 words
A method of preparing a composition for providing systemic immunity in an animal, the method comprising the step of encapsulating effective amounts of an antigen in a biocompatible excipient to form microcapsules having a size less than approximately 5 micrometers wherein said microcapsules are to be administered to said animal.
Claim 64
Independent Claim
50 words
A method of preparing a composition for providing systemic immunity in an animal, the method comprising the step of encapsulating effective amounts of an antigen in a biocompatible excipient to form microcapsules having a size less than approximately 5 micrometers wherein said microcapsules are to be administered to said animal.
Claim 65
Independent Claim
52 words
A method of increasing the bioavailability of a bioactive agent to an animal comprising the steps of:- (a) encapsulating effective amounts of said agent in a biocompatible excipient to form microcapsules having a size less than approximately 10 micrometers; and (b) administering an effective amount of said microcapsules to said animal orally.
Claim 66
Independent Claim
52 words
A method of increasing the bioavailability of a bioactive agent to an animal comprising the steps of:- (a) encapsulating effective amounts of said agent in a biocompatible excipient to form microcapsules having a size less than approximately 10 micrometers; and (b) administering an effective amount of said microcapsules to said animal orally.
Claim 67
Independent Claim
44 words
A method for delivering a bioactive agent to an animal to initiate an immune response the method comprising the step of parenterally administering to said animal a bioactive agent encapsulated in a biocompatible excipient forming a microcapsule having a size less than 10 micrometers.
Claim 68
Independent Claim
44 words
A method for delivering a bioactive agent to an animal to initiate an immune response the method comprising the step of parenterally administering to said animal a bioactive agent encapsulated in a biocompatible excipient forming a microcapsule having a size less than 10 micrometers.
Claim 69
Dependent Claim
19 words
The method of Claim 52 wherein said step of parentally administering comprises a single injection or comprises multiple injections.
References Cited (Backward Citations)
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