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EP0347839A2 Expired / Lapsed EP ⏱️ 0.0 years remaining

Dry-type analytical element for immunoassay

Assignee: Fujirebio Inc
•
Filing: 1989-06-20
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Granted: —
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Expires: 2009-06-20
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Abstract

Abstract A dry-type analytical element for immunoassay having at least one water-permeable layer for measuring a ligand in a sample according to enzyme immunoassay, which comprises, (A) a water-insoluble macromolecular substrate, and (B) an antibody, reacting with the ligand in the sample, conjugated with an enzyme capable of acting on the above water-insoluble macromolecular substrate. The above analytical substance further comprises, (C) a macromolecular substance which is a conjugate of the ligand or its derivative with a macromolecular compound in the above water-permeable layer of the above dry-type analytical element. The invention can conduct a highly sensitive and simple enzyme immunoassay.

Inventors

  • 👤 Yukio C/O Fuji Photo Film Co.Ltd. Sudo
  • 👤 Toshikage C/O Fuji Photo Film Co.Ltd. Hiraoka
  • 👤 Shigeki C/O Fuji Photo Film Co.Ltd. Kageyama
  • 👤 Yoshihiro C/O Fujirebio K.K. Ashihara
  • 👤 Isao C/O Fujirebio K.K. Nishizono
  • 👤 Tetsuji C/O Fujirebio K.K. Tanimoto

Bibliographic Details

Application Number —
Jurisdiction EP
Data Source google_patents
Last Synchronized 2026-11-09 07:13
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54366; G01N33/54386

Claims Analysis

Total Claims: 5
Claim 1
Independent Claim 55 words
A dry-type analytical element for immunoassay having at least one water-permeable layer for measuring a ligand in a liquid sample according to enzyme immunoassay, which comprises, (A) a water-insoluble macromolecular substrate, and (B) an antibody, reacting with the ligand in the sample, conjugated with an enzyme capable of acting on the above water-insoluble macromolecular substrate.
Claim 2
Dependent Claim 55 words
A dry-type analytical element for immunoassay having at least one water-permeable layer for measuring a ligand in a liquid sample according to enzyme immunoassay, which comprises, (A) a water-insoluble macromolecular substrate, and (B) an antibody, reacting with the ligand in the sample, conjugated with an enzyme capable of acting on the above water-insoluble macromolecular substrate.
Claim 3
Dependent Claim 38 words
The analytical element of claim 1 which further comprises (C) a macromolecular substance which is a conjugate of the ligand or its derivative with a macromolecular compound in the above water-permeable layer of the above dry-type analytical element.
Claim 4
Dependent Claim 27 words
The analytical element of claim 1 or claim 2 having at least two water-permeable layers and at least one of the water-permeable layers being a porous layer.
Claim 5
Dependent Claim 29 words
The analytical element of claim 1 or claim 2 which further comprises an another antibody specific to different antigenic determinant from that to the antibody conjugated with the enzyme.

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