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EP0353985A2 Expired / Lapsed EP ⏱️ 0.0 years remaining

Nucleic acid probe for detection of neisseria gonorrhoea

Assignee: Ortho Clinical Diagnostics Inc
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Filing: 1989-08-01
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Granted: —
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Expires: 2009-08-01
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Abstract

Abstract The invention discloses nucleic acid probes specific for Neisseria gonorrhoea useful in diagnostic methods to detect the presence of N. gonorrhoea in biological specimens.

Inventors

  • 👤 Derek Woods
  • 👤 Jane M. Madonna
  • 👤 Linda S. Mulcahy

Bibliographic Details

Application Number —
Jurisdiction EP
Data Source google_patents
Last Synchronized 2026-11-01 21:15
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6876; C12Q1/6888; C12Q1/689; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/871

Claims Analysis

Total Claims: 15
Claim 1
Independent Claim 32 words
1 . A nucleic acid probe, comprising all or a part of at least one nucleotide sequence selected from the group consisting of 5′...AGCTTTTTGGCGCTGCGTCCGGCTAACTGATATCTGCATGGAGGCAACCGGC AGTTATTATGAAGAAGTTGCCGACTACTTCGCGCAGTATTACAGCGTTTACGT AGTGAACCCGCTGAAAATAAGCAAGTATGCAGAAAGCAGGTTCAAGCGAACC A ACAGACAAACAGGATGCAAAAGCTGATAGCGCTCAGTATTGCCGGTCGCGGAAAGA AAGCGAGCTTGTAAAGAGGCAGAAGCTACGGACACGAGCAATACAGGCTTTTACGG ATGACGCCAGCTAACGCGTCAATCAAAGCGTAGCGCTGCATGAAACGTCTACAGGC TAAGATAG...3′ and
Claim 2
Dependent Claim 32 words
1 . A nucleic acid probe, comprising all or a part of at least one nucleotide sequence selected from the group consisting of 5′...AGCTTTTTGGCGCTGCGTCCGGCTAACTGATATCTGCATGGAGGCAACCGGC AGTTATTATGAAGAAGTTGCCGACTACTTCGCGCAGTATTACAGCGTTTACGT AGTGAACCCGCTGAAAATAAGCAAGTATGCAGAAAGCAGGTTCAAGCGAACC A ACAGACAAACAGGATGCAAAAGCTGATAGCGCTCAGTATTGCCGGTCGCGGAAAGA AAGCGAGCTTGTAAAGAGGCAGAAGCTACGGACACGAGCAATACAGGCTTTTACGG ATGACGCCAGCTAACGCGTCAATCAAAGCGTAGCGCTGCATGAAACGTCTACAGGC TAAGATAG...3′ and
Claim 3
Dependent Claim 17 words
5′..ATTCCCGGGGATCGTAATCTCCGCCTTTCTTATGTACGTGATACGCAATAAC GGCGAGTTTACGCATCAATGCTGCGATGATGACTTTTTTAGGCTTCTTCTTTT CTTCCAGTCTTGCTATGAAGTCGGGAAATGCCCTTATGCGGTATGCGACATGGCCGG CATAAACAAGACGGCGCGTAATTTCCTGTTGCCAAACTTGGTCAGTTTGCCTTTTCC CCTTACGCTTGTCCCGGATTGACTTTTTGTTGCGGGCTTAAGCTGCGAACGCTGCAA ATTTGTTTGATGTTTCAAATTTCGAAGATGTTAGATGATGAAACACTAGCTGCGTC ATTCTGCTATGCGGTATGTTCAGAGCTCAGCTCTGACGTAGCTTCG...3′; said probe capable of hybridizing to Neisseria gonorrhea chromosomal DNA.
Claim 4
Dependent Claim 17 words
5′..ATTCCCGGGGATCGTAATCTCCGCCTTTCTTATGTACGTGATACGCAATAAC GGCGAGTTTACGCATCAATGCTGCGATGATGACTTTTTTAGGCTTCTTCTTTT CTTCCAGTCTTGCTATGAAGTCGGGAAATGCCCTTATGCGGTATGCGACATGGCCGG CATAAACAAGACGGCGCGTAATTTCCTGTTGCCAAACTTGGTCAGTTTGCCTTTTCC CCTTACGCTTGTCCCGGATTGACTTTTTGTTGCGGGCTTAAGCTGCGAACGCTGCAA ATTTGTTTGATGTTTCAAATTTCGAAGATGTTAGATGATGAAACACTAGCTGCGTC ATTCTGCTATGCGGTATGTTCAGAGCTCAGCTCTGACGTAGCTTCG...3′; said probe capable of hybridizing to Neisseria gonorrhea chromosomal DNA.
Claim 5
Dependent Claim 48 words
The nucleic acid probe of claim 1 wherein said nucleic acid probe comprises a subset, derivative, subclone or mutation of said at least one nucleotide sequence; said probe capable of hybridizing to at least one insert obtained from deposited recombinant DNA having ATCC accession numbers 53409 and 53411.
Claim 6
Dependent Claim 22 words
The nucleic acid probe of claim 1 comprising a nucleic acid sequence that is complementary to said at least one nucleotide sequence.
Claim 7
Dependent Claim 18 words
The nucleic acid probe of claim 1 wherein said nucleotide sequence comprises about 20 to about 100 nucleotides.
Claim 8
Dependent Claim 21 words
A nucleic acid probe comprising all or a part of at least one nucleotide sequence selected from the group consisting of
Claim 9
Dependent Claim 21 words
A nucleic acid probe comprising all or a part of at least one nucleotide sequence selected from the group consisting of
Claim 10
Dependent Claim 24 words
The nucleic acid probe of claim 5 comprising all or a part of at least one nucleotide sequence selected from the group consisting of
Claim 11
Dependent Claim 20 words
The nucleic acid probe of claim 6 comprising a nucleotide sequence substantially complementary to said at least one nucleotide sequence.
Claim 12
Dependent Claim 49 words
The nucleic acid probe of claim 6 comprising a subset, derivative, subclone or mutation of all or a part of said at least one nucleotide sequence; said probe capable of hybridizing to at least one DNA insert obtained from deposited recombinant DNA having ATCC accession numbers 53409 and 53411.
Claim 13
Dependent Claim 20 words
The nucleic acid probe of any preceding claim further comprising a 3′ tail sequence having a detectable label bound thereon.
Claim 14
Dependent Claim 15 words
The nucleic acid probe of claim 9 wherein said tail sequence comprises a nucleotide sequence.
Claim 15
Dependent Claim 15 words
The nucleic acid probe of claim 10 wherein said nucleotide sequence comprises adenosine and uridine.

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