Patent EP0455424A2 successfully refreshed from Google Patents.
EP0455424A2
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EP
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Mammalian inducible promoter cascade system
Assignee: Merck and Co Inc
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Filing: 1991-04-25
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Granted: —
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Expires: 2011-04-25
Abstract
Abstract The present invention relates to the development of a novel mammalian inducible and cascading gene expression system. Specifically, this invention relates to the development of stable host cell lines, harboring and coexpressing the retroviral transactivator protein (TAT) under the regulation of lacI/lacO, in an inducible expression system in which any cloned gene under transcriptional control of the HIV LTR promoter, can be expressed at a high level in an inducible manner.
Inventors
- 👤 Chung-Her Jenh
- 👤 Ming-Fan Law
- 👤 Melvin Silberklang
- 👤 Iii George E. Mark
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-28 06:19
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/005; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/635; C12N15/79; C12N15/85; C12N9/00; C12N9/14; C12N9/48; C12N9/50; C12N9/64; C12N9/6421; C12N9/6424; C12N9/6456; C12N2740/00; C12N2740/00011; C12N2740/10011; C12N2740/16011; C12N2740/16311; C12N2740/16322; C12N2830/00; C12N2830/15; C12N2830/60; C12N2840/00; C12N2840/10; C12N2840/105
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
19 words
A plasmid which contains DNA encoding HIV TAT protein which is transcriptionally regulated by DNA encoding the lac operator.
Claim 2
Dependent Claim
29 words
A plasmid containing HIV LTR promoter DNA controlling transcription of a cloned foreign gene, which can be trans-activated by TAT protein, for use in an inducible gene expression system.
Claim 3
Dependent Claim
19 words
The plasmid according to Claim 2, in which the cloned foreign gene consists of DNA encoding bat plasminogen activator.
Claim 4
Dependent Claim
28 words
A recombinant mammalian cell line which expresses the lac repressor protein and contains DNA encoding HIV TAT protein which is transcriptionally regulated by DNA encoding the lac operator.
Claim 5
Dependent Claim
51 words
A recombinant mammalian cell line which: a) expresses the lac repressor protein; b) contains DNA encoding HIVTAT protein which is regulated by DNA encoding the lac operator; and c) contains DNA comprising the HIV LTR promoter controlling transcription of DNA encoding bat plasminogen activator, which can be trans-activated by TAT protein.
Claim 6
Dependent Claim
120 words
An inducible gene expression system for the controlled expression of cloned genes in mammalian cells comprising: a) a recombinant mammalian cell line which expresses a transcription repressor protein, and contains DNA encoding a transactivator protein which is regulated by DNA encoding the target for the transcription repressor protein, b) a plasmid introduced into said recombinant cell line, said plasmid containing a promoter controlling transcription of the cloned foreign gene, which is capable of transactivation by the transactivator protein, resulting in an inducible gene expression system, c) said system being inducible for expression of the cloned gene by addition of an inducer, which inducer causes the derepression of the transactivator protein expression and subsequent trans-activation of the transactivatable promoter of (b).
Claim 7
Dependent Claim
116 words
An inducible gene expression system for the controlled expression of cloned genes in mammalian cells comprising: a) a recombinant mammalian cell line which expresses lac repressor protein, and contains DNA encoding a transactivator protein which is regulated by DNA encoding the lac operator, b) a plasmid introduced into said recombinant cell line, said plasmid containing a promoter controlling transcription of the cloned foreign gene, which is capable of transactivation by the transactivator protein, resulting in an inducible gene expression system, c) said system being inducible for expression of the cloned gene by addition of a chemical inducer, which inducer causes the derepression of the transactivator protein expression and subsequent trans-activation of the transactivatable promoter of (b).
Claim 8
Dependent Claim
111 words
An inducible gene expression system for the controlled expression of cloned genes in mammalian cells comprising: a) a recombinant mammalian cell line which expresses lac repressor protein, and contains DNA encoding HIVTAT protein which is regulated by DNA encoding the lac operator, b) a plasmid introduced into said recombinant cell line, said plasmid containing HIV LTR promoter controlling transcription of the cloned foreign gene, which is capable of transactivation by TAT protein,resulting in an inducible gene expression system, c) said system being inducible for expression of the cloned gene by addition of a chemical inducer, which inducer causes the derepression of TAT expression and subsequent trans-activation of the HIV LTR promoter.
Claim 9
Dependent Claim
16 words
The recombinant mammalian cell line according to Claim 3, with the ATCC deposit accession number CRL-l0404.
Claim 10
Dependent Claim
16 words
The recombinant mammalian cell line according to Claim 4, with the ATCC deposit accession number CRL-l0405.
References Cited (Backward Citations)
EP0352119A2
Cited
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