§
PatentCue
Patent Intelligence
Join Waitlist Sign In
Patent EP0550296A2 successfully refreshed from Google Patents.
← Back to Patent Explorer
EP0550296A2 Expired / Lapsed EP ⏱️ 0.0 years remaining

Vascular endothelial cells growth factor

Assignee: Terumo Corp
•
Filing: 1992-11-27
•
Granted: —
•
Expires: 2012-11-27
📄 Original PDF 🌐 Google Patents 🔄 Sync Now

Abstract

Abstract A novel protein of human origin produced by a human ovarian tumor established cell line HUOCA-II or HUOCA-III, which has a molecular weight, when determined by SDS-polyacrylamide gel electrophoresis, of from 72,000 to 80,000 daltons under a non-reducing condition or from 79,000 to 85,000 daltons under a reducing condition, which contains an amino acid sequence represented by the Sequence ID No. 4 deduced from the DNA sequence represented by the Sequence ID No. 5, and which enhances growth of vascular endothelial cells but does not activate growth of smooth muscle cells, fibroblasts and hepatocytes and also does not enhance or inhibit growth of HeLa cells. This invention also provides a process for the production of the protein.

Inventors

  • 👤 Tadashi Sudo
  • 👤 Kazumichi Harada
  • 👤 Ichiro Hirahara
  • 👤 Masami Adachi

Bibliographic Details

Application Number —
Jurisdiction EP
Data Source google_patents
Last Synchronized 2026-10-29 07:16
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/475; A; A61; A61K; A61K38/00

Claims Analysis

Total Claims: 10
Claim 1
Independent Claim 54 words
A single chain protein selectively enhancing the growth of vascular endothelial cells, characterized in that it comprises the following peptide chains : and in that it has a molecular weight of from 72,000 to 80,000 Da when determined by SDS polyacrylamide gel electrophoresis or from 79,000 to 85,000 Da when determined under reducing conditions.
Claim 2
Dependent Claim 56 words
A process for producing the protein according to claim 1 which comprises purifying a serum-free culture supernatant of said human ovarian tumor established cell line, HUOCA-II or HUOCA-III, by combining purification techniques including (a) cation exchange chromatography, (b) heparin affinity chromatography, (c) heparin affinity high performance liquid chromatography and (d) reverse phase high performance liquid chromatography.
Claim 3
Dependent Claim 30 words
A protein of human origin which contains an amino acid sequence or a portion of the amino acid sequence represented by the following sequence (SEQ ID No. : 4) :
Claim 4
Dependent Claim 16 words
A pharmaceutical composition which contains the protein of claim 1 or 3 as an active ingredient.
Claim 5
Dependent Claim 37 words
A DNA fragment which contains a nucleotide sequence or a portion of the nucleotide sequence below (SEQ ID No. : 5) : wherein at least one base may be substituted based on the degeneracy of genetic code.
Claim 6
Dependent Claim 23 words
A single chain protein having an activity to enhance the growth of vascular endothelial cells obtainable from the DNA fragment of claim 5.
Claim 7
Dependent Claim 11 words
A DNA fragment complementary to the DNA fragment of claim 5.
Claim 8
Dependent Claim 11 words
An expression vector which contains the DNA fragment of claim 5.
Claim 9
Dependent Claim 10 words
A transformant transformed with the DNA fragment of claim 5.
Claim 10
Dependent Claim 10 words
A transformant transformed with the expression vector of claim 8.

References Cited (Backward Citations)

No citation records indexed.

USPTO Annuity & Maintenance Windows

Maintenance schedule is calculated for granted US utility patents.

Assigned Categories & Tags

No categories assigned to this patent yet.

Assign Category Tag