Patent US2117459A successfully refreshed from Google Patents.
Patent EP0599337A2 successfully refreshed from Google Patents.
EP0599337A2
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EP
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Method for detection of nucleic acid and probe therefor
Assignee: Canon Inc
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Filing: 1993-11-26
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Granted: —
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Expires: 2013-11-26
Abstract
Abstract A method for detecting a nucleic acid hybrid comprises steps of adding a nucleic acid probe into a sample solution containing a targeted nucleic acid, and detecting a double helical structure of a hybrid formed between the probe and the targeted nucleic acid, wherein the step for detecting the double helical structure comprises incorporating, into the sample solution, two or more kinds of reagents which are capable of causing a detectable change by interaction therebetween through the double helical structure and measuring the change caused by the interaction of the reagents; and at least one of the two or more kinds of reagents is joined to the probe.
Inventors
- 👤 Nobuko C/O Canon Kabushiki Kaisha Yamamoto
- 👤 Tadashi C/O Canon Kabushiki Kaisha Okamoto
- 👤 Yoshinori C/O Canon Kabushiki Kaisha Tomida
- 👤 Masahiro C/O Canon Kabushiki Kaisha Kawaguchi
- 👤 Keisuke C/O Canon Kabushiki Kaisha Makino
- 👤 Akira C/O Canon Kabushiki Kaisha Murakami
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-29 05:53
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6813; C12Q1/6816; C12Q1/6818
Claims Analysis
Total Claims: 55
Claim 1
Independent Claim
105 words
A method for detecting a nucleic acid hybrid comprising steps of adding a nucleic acid probe into a sample solution containing a targeted nucleic acid, and detecting a double helical structure of a hybrid formed between the probe and the targeted nucleic acid: said step for detecting the double helical structure comprising incorporating, into the sample solution, two or more reagents which are capable of causing a detectable change by interaction therebetween through the double helical structure, and measuring the change caused by the interaction of the reagents; and at least one of said two or more kinds of reagents being joined to the probe.
Claim 2
Dependent Claim
20 words
The method according to Claim 1, wherein the interaction between the two or more reagents is based on charge transfer.
Claim 3
Dependent Claim
22 words
The method according to Claim 2, wherein the two or more reagents include at least an electron donor and an electron acceptor.
Claim 4
Dependent Claim
21 words
The method according to Claim 2, wherein the charge transfer is effected through stacked base pairs constructing the double helical structure.
Claim 5
Dependent Claim
34 words
The method according to Claim 3, wherein the charge transfer is effected based on an effect of proximity between the electron donor and the electron acceptor resulting from formation of the double helix formation.
Claim 6
Dependent Claim
26 words
The method according to Claim 3, wherein the electron acceptor is a substance which causes change in EPR (electron spin resonance) spectrum on accepting an electron.
Claim 7
Dependent Claim
21 words
The method according Claim 1 or Claim 2, wherein the change caused by the interaction between the reagents is detected optically.
Claim 8
Dependent Claim
21 words
The method according Claim 1 or Claim 2, wherein the change caused by the interaction between the reagents is detected chemically.
Claim 9
Dependent Claim
25 words
The method according to Claim 1 or Claim 2, wherein the reagent is an intercalater for the nucleic acid base constructing the double helical structure.
Claim 10
Dependent Claim
17 words
The method according to Claim 3, wherein the interaction between the reagents is initiated by light irradiation.
Claim 11
Independent Claim
89 words
A method for detecting a nucleic acid hybrid comprising steps of adding a nucleic acid probe into a sample solution containing a targeted nucleic acid, and detecting a double helical structure of a hybrid formed between the probe and the targeted nucleic acid: said step for detecting the double helical structure comprising incorporating, into the sample solution, a reagent which is capable of causing a detectable change by interaction with the double helical structure, measuring the change caused by the interaction of the reagent with the double helical structure.
Claim 12
Dependent Claim
14 words
The method according to Claim 11, wherein the interaction is based on charge transfer.
Claim 13
Dependent Claim
22 words
The method according to Claim 12, wherein the reagent is an electron donor or an electron acceptor to the double helical structure.
Claim 14
Dependent Claim
21 words
The method according to Claim 12, wherein the charge transfer is effected through stacked base pairs constructing the double helical structure.
Claim 15
Dependent Claim
34 words
The method according to Claim 13, wherein the charge transfer is effected based on an effect of proximity between the electron donor and the electron acceptor resulting from formation of the double helical structure.
Claim 16
Dependent Claim
26 words
The method according to Claim 13, wherein the electron acceptor is a substance which causes change in EPR (electron spin resonance) spectrum on accepting an electron.
Claim 17
Dependent Claim
15 words
The method according Claim 11, wherein the change caused by the interaction is detected optically.
Claim 18
Dependent Claim
15 words
The method according Claim 11, wherein the change caused by the interaction is detected chemically.
Claim 19
Dependent Claim
25 words
The method according to Claim 11 or Claim 12, wherein the reagent is an intercalater for the nucleic acid base constructing the double helical structure.
Claim 20
Dependent Claim
14 words
The method according to Claim 13, wherein the interaction is initiated by light irradiation.
Claim 21
Dependent Claim
14 words
The method according to Claim 11, wherein the reagent is linked to the probe.
Claim 22
Dependent Claim
60 words
A probe for detection of a targeted nucleic acid, having a sequence for hybridization with the targeted nucleic acid: said probe being linked to at least one of two or more reagents which are capable of causing detectable change by interaction between the reagents through a double helical structure formed by hybridization of the targeted nucleic acid with the probe.
Claim 23
Dependent Claim
27 words
The probe for detection of a targeted nucleic acid according to Claim 22, wherein the interaction between the two or more reagents is based on charge transfer.
Claim 24
Dependent Claim
29 words
The probe for detection of a targeted nucleic acid according to Claim 23, wherein the two or more reagents include at least an electron donor and an electron acceptor.
Claim 25
Dependent Claim
28 words
The probe for detection of a targeted nucleic acid according to Claim 23, wherein the charge transfer is effected through stacked base pairs constructing the double helical structure.
Claim 26
Dependent Claim
38 words
The probe for detection of a targeted nucleic acid according to Claim 24, wherein the charge transfer is effected based on an effect of proximity between the electron donor and the electron acceptor through the double helical structure.
Claim 27
Dependent Claim
33 words
The probe for detection of a targeted nucleic acid according to Claim 24, wherein the electron acceptor is a substance which causes change in EPR (electron spin resonance) spectrum on accepting an electron.
Claim 28
Dependent Claim
25 words
The probe for detection of a targeted nucleic acid according Claim 22, wherein the change caused by the interaction between the reagents is detected optically.
Claim 29
Dependent Claim
25 words
The probe for detection of a targeted nucleic acid according Claim 22, wherein the change caused by the interaction between the reagents is detected chemically.
Claim 30
Dependent Claim
32 words
The probe for detection of a targeted nucleic acid according to Claim 22 or Claim 23, wherein the reagent is an intercalater for the nucleic acid base constructing the double helical structure.
Claim 31
Dependent Claim
24 words
The probe for detection of a targeted nucleic acid according to Claim 23, wherein the interaction between the reagents is initiated by light irradiation.
Claim 32
Dependent Claim
51 words
A probe for detection of a nucleic acid hybrid, having a sequence for hybridization with the targeted nucleic acid: said probe being linked to a reagent which is capable of causing detectable change by interaction with a double helical structure formed by hybridization of the targeted nucleic acid with the probe.
Claim 33
Dependent Claim
14 words
The probe according to Claim 32, wherein the interaction is based on charge transfer.
Claim 34
Dependent Claim
17 words
The probe according to Claim 33, wherein the reagent is an electron donor or an electron acceptor.
Claim 35
Dependent Claim
21 words
The probe according to Claim 33, wherein the charge transfer is effected through stacked base pairs constructing the double helical structure.
Claim 36
Dependent Claim
34 words
The probe according to Claim 34, wherein the charge transfer is effected based on an effect of proximity between the electron donor and the electron acceptor resulting from formation of the double helical structure.
Claim 37
Dependent Claim
26 words
The probe according to Claim 34, wherein the electron acceptor is a substance which causes change in EPR (electron spin resonance) spectrum on accepting an electron.
Claim 38
Dependent Claim
15 words
The probe according Claim 32, wherein the change caused by the interaction is detected optically.
Claim 39
Dependent Claim
15 words
The probe according Claim 32, wherein the change caused by the interaction is detected chemically.
Claim 40
Dependent Claim
25 words
The probe according to Claim 32 or Claim 33, wherein the reagent is an intercalater for the nucleic acid base constructing the double helical structure.
Claim 41
Dependent Claim
14 words
The probe according to Claim 33, wherein the interaction is initiated by light irradiation.
Claim 42
Independent Claim
65 words
A method of detection of a targeted nucleic acid, comprising addition of a probe having at least two pigments linked thereto into a sample solution, and detection of a double helical structure of a hybrid formed between the probe and the targeted nucleic acid in the sample solution by means of the optical change given by charge-transfer between the pigments through the double helical structure.
Claim 43
Dependent Claim
38 words
The method according to Claim 42, wherein one of the pigments is an electron donor and another one of the pigments is an electron acceptor, and the electron donor and the electron acceptor are linked to the probe.
Claim 44
Dependent Claim
24 words
The method according to Claim 42 or Claim 43, wherein the charge transfer is effected through stacked base pairs constructing the double helical structure.
Claim 45
Dependent Claim
29 words
The method according to Claims 42 or Claim 43, wherein at least one of the pigments is an intercalater for the nucleic acid base constituting the double helical structure.
Claim 46
Dependent Claim
18 words
The method according to Claim 42 or Claim 43, wherein the charge transfer is initiated by light irradiation.
Claim 47
Dependent Claim
44 words
The method according to Claim 42, wherein one of the pigments as an electron donor is linked to one terminal portion of the probe, and another one of the pigments as an electron acceptor is linked to the other terminal portion of the probe.
Claim 48
Dependent Claim
56 words
A probe for detection of a targeted nucleic acid, having a sequence for hybridization with the targeted nucleic acid: said probe being linked to two or more pigments which are capable of causing detectable optical change by charge transfer therebetween through a double helical structure formed by hybridization of the targeted nucleic acid and the probe.
Claim 49
Dependent Claim
38 words
The probe according to Claim 48, wherein one of the pigments is an electron donor and another one of the pigments is an electron acceptor, and the electron donor and the electron acceptor are linked to the probe.
Claim 50
Dependent Claim
21 words
The probe according to Claim 48, wherein the charge transfer is effected through stacked base pairs constructing the double helical structure.
Claim 51
Dependent Claim
29 words
The probe according to Claim 48 or Claim 49, wherein at least one of the pigments is an intercalater for the nucleic acid base constituting the double helical structure.
Claim 52
Dependent Claim
19 words
The probe according to any of Claims 48 to 50, wherein the charge transfer is initiated by light irradiation.
Claim 53
Dependent Claim
44 words
The method according to Claim 48, wherein one of the pigments as an electron donor is linked to one terminal portion of the probe, and another one of the pigments as an electron acceptor is linked to the other terminal portion of the probe.
Claim 54
Dependent Claim
26 words
The method according to Claim 44, wherein at least one of the pigments is an intercalater for the uncleic acid base constituting the double helical structure.
Claim 55
Dependent Claim
15 words
The method according to Claim 44, wherein the charge transfer is initiated by light irradiation.
References Cited (Backward Citations)
No citation records indexed.
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