Abstract The invention provides a method for quantitative
measurement of a substrate with high accuracy by
electrochemically oxidizing an electron mediator which has
been reduced by enzyme reaction thereby determining the
substrate concentration based on a current flowing during
electrochemical oxidation from which adverse effects of an
easy-to-oxidize substance on the oxidation process have
been minimized. The quantitating method in accordance
with the present invention comprises a first step for
causing a substrate contained in a sample to react with a
specific oxidoreductase to the substrate in the presence
of an electron mediator in oxidized state, and a second
step for electrochemically reducing the electron mediator
in oxidized state which remains non-reduced by the enzyme
reaction in the first step, thereby obtaining a current
flowing during electrochemical reduction.
A method for quantitative measurement of a
substrate comprising: a first step for causing a substrate contained
in a sample to react with a specific oxidoreductase to
said substrate in the presence of an electron mediator in
oxidized state, and a second step for electrochemically reducing
said electron mediator in oxidized state which remains
non-reduced by enzyme reaction in said first step, thereby
obtaining a current flowing during electrochemical
reduction.
Claim 2
Dependent Claim
30 words
The method for quantitative measurement of a
substrate in accordance with claim 1, wherein an electron
mediator in reduced state further participates in the
enzyme reaction in said first step.
Claim 3
Dependent Claim
66 words
The method for quantitative measurement of a
substrate in accordance with claim 1, wherein said
quantitative measurement is conducted using a biosensor
comprising an electrically insulating base plate, an
electrode system having at least a working electrode and a
counter electrode which are formed on said base plate, and
a reaction layer including an oxidoreductase and an
electron mediator which are disposed on said electrode
system.
Claim 4
Dependent Claim
69 words
The method for quantitative measurement of a
substrate in accordance with claim 1, wherein said
quantitative measurement is conducted using a biosensor
comprising an electrically insulating base plate, an
electrode system having at least a working electrode, a
counter electrode and a reference electrode which are
formed on said base plate, and a reaction layer including
an oxidoreductase and an electron mediator which are
disposed on said electrode system.
Claim 5
Dependent Claim
22 words
The method for quantitative measurement of a
substrate in accordance with claim 3, wherein said
reaction layer further includes a hydrophilic polymer.
Claim 6
Dependent Claim
22 words
The method for quantitative measurement of a
substrate in accordance with claim 4, wherein said
reaction layer further includes a hydrophilic polymer.
Claim 7
Dependent Claim
30 words
The method for quantitative measurement of a
substrate in accordance with claim 3, wherein an electron
mediator in reduced state further participates in the
enzyme reaction in said first step.
Claim 8
Dependent Claim
30 words
The method for quantitative measurement of a
substrate in accordance with claim 4, wherein an electron
mediator in reduced state further participates in the
enzyme reaction in said first step.
References Cited (Backward Citations)
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