Abstract A biosensor which enables rapid and simple
quantitation of a substrate with high accuracy and which
demonstrates an excellent preservation characteristic by
best retaining enzyme activity is disclosed. The
biosensor comprises an electrically insulating base plate,
an electrode system including at least a working electrode
and a counter electrode formed on the base plate, and a
reaction layer containing at least an enzyme and a sugar
formed on the electrode system, wherein the enzyme is at
least one selected from the group consisting of glucose
oxidase, glucose dehydrogenase and fructose dehydrogenase
and the sugar is at least one selected from the group
consisting of trehalose, sucrose, glycerol, mannitol and
ribose. When the enzyme is glucose dehydrogenase whose
coenzyme is pyrrolo-quinoline quinone, the use of
trehalose as sugar is particularly preferable.
Inventors
👤 Keiko Yugawa
👤 Toshihiko Yoshioka
👤 Shiro Nankai
Bibliographic Details
Application Number—
JurisdictionEP
Data Sourcegoogle_patents
Last Synchronized2026-11-03 08:56
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/001; C12Q1/005
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
82 words
A biosensor comprising: an electrically insulating base plate, an electrode system including at least a working
electrode and a counter electrode formed on said base
plate, and a reaction layer containing at least an enzyme
and a sugar formed on said electrode system, wherein said enzyme is at least one selected
from the group consisting of glucose oxidase, glucose
dehydrogenase and fructose dehydrogenase and said sugar is
at least one selected from the group consisting of
trehalose, sucrose, glycerol, mannitol and ribose.
Claim 2
Dependent Claim
23 words
The biosensor in accordance with claim 1,
wherein said enzyme is glucose dehydrogenase whose
coenzyme is pyrrolo-quinoline quinone and said sugar is
trehalose.
Claim 3
Dependent Claim
32 words
The biosensor in accordance with claim 2,
wherein said reaction layer contains said glucose
dehydrogenase 1 to 40 units per sensor chip and said
trehalose 4 to 400 nM per sensor chip.
Claim 4
Dependent Claim
32 words
The biosensor in accordance with claim 2,
wherein said reaction layer contains said glucose
dehydrogenase 5 to 20 units per sensor chip and said
trehalose 40 to 200 nM per sensor chip.
References Cited (Backward Citations)
No citation records indexed.
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