Microbiological production of carotene in a medium comprising kerosene

Claim 1

IN THE METHOD OF PRODUCING B-CAROTENE BY FERMENTING ON ORGANISM FROM THE GROUP CONSISTING OF BLAKESLEA TRISPORA NRRL 9159, BLAKESLEA TRISPORA NRRL 9216, AND MIXTURES THEREOF IN A TAP WATER FERMENTATION MEDIUM COMPRISING NEUTRALIZED CEREAL GRAIN HYDROLYSTATE, WHITE GREASE, A NONIONIC DETERGENT, THIAMINE, AND B-IONONE, THE IMPROVEMENT COMRPISING THE STEPS OF ADDING ABOUT 5% BY VOLUME (BASED ON THE FERMENTAION MEDIUM) OF A PETROLEUM HYDROCARBON FRACTION AT UP TO ABOUT 48 HOURS AFTER BEGINNING THE FERMENTATION, SAID PETROLEUM HYDROCARBON FRACTION BEING SELECTED FROM THE GROUP CONSISTING OF KEROSENE AND KEROSENE FRACTIONS CHARACTERIZED BY HAVING AN INITIAL B.P. OF 376-386* F., AND END B.P. OF 415484* F., A SP. GR. AT 60* F. OF 0.775-0.789, AND API GRAVITY OF 47.7-49.8, A REFRACTIVE INDEX AT 50* C. OF 1,43221,4360, A CLOSED CUP FLASH POINT OF 135-150* F., A KAURI BUTANOL NUMBER OF 29-31, AN ANILINE POINT OF 161-175* F., AND IODINE NUMBER OF 0-10, AND CONTINUING THE FERMENTATION TO A TOTAL PERIOD OF ABOUT 144 HOURS.