Patent US3867255A successfully refreshed from Google Patents.
US3867255A
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Process of making yeast protein isolate having reduced nucleic acid content
Assignee: Anheuser Busch Companies LLC
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Filing: 1972-11-29
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Granted: 1975-02-18
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Expires: 1992-11-29
Abstract
Abstract This disclosure relates to a process of making a yeast protein isolate of low nucleic acid content. The process involves adding exogenous nuclease to a solubles fraction separated from a mass of ruptured yeast cells to hydrolyze the nucleic acid. The preferred source of exogenous nuclease is malt sprouts.
Inventors
- 👤 Jon Albert Newell
- 👤 Robert Dudley Seeley
- 👤 Ernest Aleck Robbins
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 20:19
CPC Classifications
C; C12; C12N; C12N1/00; C12N1/005; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/911; Y10S435/921; Y10S435/94; Y10S435/942; Y10S530/00; Y10S530/82; Y10S530/821; Y10S530/823; Y10S530/824
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
85 words
A PROCESS FOR PRODUCING A YEAST PROTEIN PRODUCT COMPRISING THE STEPS OF: A. RUPTURING YEAST CELLS, B. SEPARATING A SOLUBLES FRACTION CONTAINIG NUCLE ACID AND PROTEIN FROM AN INSOLUBLE FRACTION, C. ADDING NUCLES TO THE SOLUBLES FRACTION, D. HYDROLYZING THE NUCLEIC ACID WITH THE NUCLEASE AT A PH OF ABOUT 5 TO ABOUT 10 AND A TEMPERATURE OF ABOUT 30* TO ABOUT 60*C. FOR UP TO FOUR HOURS, AND E. SEPARATING AN INSOLUBLE FRACTION CONTAINING SAID PROTEIN FROM A SOLUBLE FRACTION CONTAINING SAID NUCLEIC ACID.
Claim 2
Dependent Claim
85 words
A PROCESS FOR PRODUCING A YEAST PROTEIN PRODUCT COMPRISING THE STEPS OF: A. RUPTURING YEAST CELLS, B. SEPARATING A SOLUBLES FRACTION CONTAINIG NUCLE ACID AND PROTEIN FROM AN INSOLUBLE FRACTION, C. ADDING NUCLES TO THE SOLUBLES FRACTION, D. HYDROLYZING THE NUCLEIC ACID WITH THE NUCLEASE AT A PH OF ABOUT 5 TO ABOUT 10 AND A TEMPERATURE OF ABOUT 30* TO ABOUT 60*C. FOR UP TO FOUR HOURS, AND E. SEPARATING AN INSOLUBLE FRACTION CONTAINING SAID PROTEIN FROM A SOLUBLE FRACTION CONTAINING SAID NUCLEIC ACID.
Claim 3
Dependent Claim
16 words
The process of claim 1 wherein the yeast cells are ruptured by homogenization below about 50*C.
Claim 4
Dependent Claim
45 words
The process of claim 1 including the step of extracting the ruptured yeast cells at a pH between 6 and 11 and a temperature between 25*C. and 60*C. for 5 to 60 minutes before separating into the solubles fraction and the insoluble cell wall fractions.
Claim 5
Dependent Claim
27 words
The process of claim 3 wherein the ruptured yeast cells are extracted at pH 9.5 at a temperature between 25*C. and 60*C. for 5 to 60 minutes.
Claim 6
Dependent Claim
19 words
The process of claim 3 wherein the pH yeast cells are extracted at ph 9.5, 60*C. for 30 minutes.
Claim 7
Dependent Claim
42 words
The process of claim 3 wherein the solubles fraction after the separation of the cell wall debris is treated with the nuclease preparation at pH 5 to pH 10, at temperatures between 30* and 60*C., for a time less than four hours.
Claim 8
Dependent Claim
29 words
The process of claim 6 wherein the protein fraction is separated from the nucleic acid fraction by centrifugation at pH 4.5-8.0 at a temp. of not greater than 60*C.
Claim 9
Dependent Claim
13 words
The process of claim 7 wherein the nuclease is derived from malt sprouts.
Claim 10
Dependent Claim
15 words
The process of claim 1 wherein the insoluble cell wall material is separated by centrifugation.
Claim 11
Dependent Claim
42 words
The process of claim 1 wherein the solubles fraction after the separation of the cell wall debris is treated with the nuclease preparation at pH 5 to pH 10, at temperatures between 30* and 60*C., for a time less than 4 hours.
Claim 12
Dependent Claim
27 words
The process of claim 10 wherein the solubles fraction after the separation of the cell wall debris is treated with the nuclease preparation at pH 7, 50*C.
Claim 13
Dependent Claim
29 words
The process of claim 1 wherein the protein fraction is separated from the nucleic acid fraction by centrifugation at pH 4.5-8.0 at a temp. of not greater than 60*C.
Claim 14
Dependent Claim
13 words
The process of claim 1 wherein the nuclease is derived from malt sprouts.
Claim 15
Dependent Claim
52 words
The process of claim 1 including the step of recovering a yeast protein product comprising: about 65% to about 85% protein; about 0.5% to about 9% RNA; about 7% to about 15% lipid; about 1 to about 5% ash; about 5 to about 20% carbohydrate; and about 0 to about 1% fiber.
Claim 16
Dependent Claim
16 words
The process of claim 14 wherein the recovered yeast protein contains less than about 5% RNA.
Claim 17
Dependent Claim
16 words
The process of claim 14 wherein the recovered yeast protein contains less than about 3% RNA.
Claim 18
Dependent Claim
16 words
The process of claim 1 wherein the yeast is selected from Candida utilis and Saccharomyces cerevisiae.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-02-17 | 1978-08-19 | 1979-02-18 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-02-17 | 1982-08-19 | 1983-02-18 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-02-17 | 1986-08-19 | 1987-02-18 | $7700.00 | Window Closed / Lapsed |