Transaminase assay

Claim 2

A reagent formulation for use in assaying a biological specimen for glutamic oxaloacetic transaminase comprising a granular, water-soluble, substantially anhydrous, storage-stable mixture containing L-aspartic acid, malate dehydrogenase, reduced nicotinamide adenine dinucleotide disodium salt, bovine serum albumin, a carbohydrate polymer, tris-(hydroxymethyl)-aminomethane, succinic acid, gum arabic, and a nitrogen-containing polyoxyalkylene nonionic surfactant obtained by the sequential reaction of ethylenediamine with propylene oxide and ethylene oxide in the presence of a catalyst, said surfactant containing polyoxypropylene chains having an average molecular weight of between about 750 and about 6,750 and polyoxyethylene chains constituting between about 10 and about 80 weight percent of said surfactant.

Claim 3

A reagent formulation as set forth in claim 1 wherein the nitrogen-containing surfactant is solid and the polyoxypropylene chains thereof have an average molecular weight of less than about 4,000.

Claim 4

A reagent formulation as set forth in claim 2 wherein the polyoxypropylene chains of the surfactant have an average molecular weight of about 2,750 and the weight percentage of the polyoxyethylene units thereof is about 70%.

Claim 5

A reagent formulation for use in assaying a biological specimen for a transaminase comprising a granular, water-soluble, substantially anhydrous, storage-stable mixture containing sodium alpha-ketoglutarate, mannitol, and a nitrogen-containing polyoxyalkylene nonionic surfactant obtained by the sequential reaction of ethylene diamine with propylene oxide and ethylene oxide in the presence of a catalyst, said surfactant containing polyoxypropylene chains having an average molecular weight of between about 750 and about 6,750 and polyoxyethylene chains constituting between about 10 and about 80 weight percent of said surfactant.

Claim 6

A reagent formulation for use in assaying a biological specimen for a transaminase comprising a granular, water-soluble, substantially anhydrous, storage-stable mixture containing sodium alpha-ketoglutarate, mannitol, and a nitrogen-containing polyoxyalkylene nonionic surfactant obtained by the sequential reaction of ethylene diamine with propylene oxide and ethylene oxide in the presence of a catalyst, said surfactant containing polyoxypropylene chains having an average molecular weight of between about 750 and about 6,750 and polyoxyethylene chains constituting between about 10 and about 80 weight percent of said surfactant.

Claim 7

A reagent formulation as set forth in claim 4 wherein the nitrogen-containing surfactant is solid and the polyoxypropylene chains thereof have an average molecular weight of less than about 4,000.

Claim 8

A reagent formulation as set forth in claim 5 wherein the polyoxypropylene chains of the surfactant have an average molecular weight of about 2,750 and the weight percentage of the polyoxyethylene units is about 70%.

Claim 9

A reagent formulation for use in assaying a biological specimen for glutamic pyruvic transaminase comprising a granular, water-soluble, substantially anhydrous, storage-stable mixture containing reduced nicotinamide adenine dinucleotide disodium salt, gum arabic, tris-(hydroxymethyl)-aminomethane, a carbohydrate polymer, bovine serum albumin, lactate, dehydrogenase, ammonium sulfate, DL-alanine, dibasic sodium phosphate, monobasic sodium phosphate, and a nitrogen-containing polyoxyalkylene nonionic surfactant obtained by the sequential reaction of ethylenediamine with propylene oxide and ethylene oxide in the presence of a catalyst, said surfactant containing polyoxypropylene chains having an average molecular weight of between about 750 and about 6,750 and polyoxyethylene chains constituting between about 10 and about 80 weight percent of said surfactant.

Claim 10

A reagent formulation for use in assaying a biological specimen for glutamic pyruvic transaminase comprising a granular, water-soluble, substantially anhydrous, storage-stable mixture containing reduced nicotinamide adenine dinucleotide disodium salt, gum arabic, tris-(hydroxymethyl)-aminomethane, a carbohydrate polymer, bovine serum albumin, lactate, dehydrogenase, ammonium sulfate, DL-alanine, dibasic sodium phosphate, monobasic sodium phosphate, and a nitrogen-containing polyoxyalkylene nonionic surfactant obtained by the sequential reaction of ethylenediamine with propylene oxide and ethylene oxide in the presence of a catalyst, said surfactant containing polyoxypropylene chains having an average molecular weight of between about 750 and about 6,750 and polyoxyethylene chains constituting between about 10 and about 80 weight percent of said surfactant.

Claim 11

A reagent formulation as set forth in claim 7 wherein the nitrogen-containing surfactant is solid and the polyoxypropylene chains thereof have an average molecular weight of less than about 4,000.

Claim 12

A reagent formulation as set forth in claim 8 wherein the polyoxypropylene chains of the surfactant have an average molecular weight of about 2,750 and the weight percentage of the polyoxyethylene units is about 70%.

Claim 15

A method as set forth in claim 10 wherein the nitrogen-containing surfactant is solid and the polyoxypropylene chains thereof have an average molecular weight of less than about 4,000.

Claim 16

A method as set forth in claim 11 wherein the polyoxypropylene chains of the surfactant have an average molecular weight of about 2,750 and the weight percentage of the polyoxyethylene units thereof is about 70%.

Claim 19

A method as set forth in claim 13 wherein the nitrogen-containing surfactant is solid and the polyoxypropylene chains thereof have an average molecular weight of less than about 4,000.

Claim 20

A method as set forth in claim 14 wherein the polyoxypropylene chains of the surfactant have an average molecular weight of about 2,750 and the weight percentage of the polyoxyethylene units thereof is about 70%.

Claim 23

A method as set forth in claim 16 wherein the nitrogen-containing surfactant is solid and the polyoxypropylene chains thereof have an average molecular weight of less than about 4,000.

Claim 24

A method as set forth in claim 17 wherein the polyoxypropylene chains of the surfactant have an average molecular weight of about 2,750 and the weight percentage of the polyoxyethylene units thereof is about 70%.

Claim 27

a coenzyme reagent formulation containing: a. L-aspartic acid; b. malate dehydrogenase; c. reduced nicotinamide adenine dinucleotide disodium salt; d. bovine serum albumin; e. a carbohydrate polymer; f. tris-(hydroxymethyl)-aminomethane; g. succinic acid; h. gum arabic; and i. a nitrogen-containing polyoxyalkylene nonionic surfactant obtained by the sequential reaction of ethylenediamine with propylene oxide and ethylene oxide in the presence of a catalyst, said surfactant containing polyoxypropylene chains having an average molecular weight of between about 750 and about 6,750 and polyoxyethylene chains constituting between about 10 and about 80 weight percent of said surfactant; 2. a substrate reagent formulation containing: a. sodium alpha-ketoglutarate; b. mannitol; and c. said nitrogen-containing polyoxyalkylene nonionic surfactant; the method comprising the steps of: i. dissolving said reagents in separate portions of water to produce liquid reagents; ii. mixing the liquid reagent containing said coenzyme reagent formulation with a biological specimen to form a specimen/reagent test system; iii. incubating said system; iv. thereafter adding the liquid reagent containing said substrate reagent formulation to said system; and v. measuring a property of said system at two different predetermined points in time following the addition of said liquid reagent containing said substrate reagent formulation.

Claim 28

a coenzyme reagent formulation containing: a. L-aspartic acid; b. malate dehydrogenase; c. reduced nicotinamide adenine dinucleotide disodium salt; d. bovine serum albumin; e. a carbohydrate polymer; f. tris-(hydroxymethyl)-aminomethane; g. succinic acid; h. gum arabic; and i. a nitrogen-containing polyoxyalkylene nonionic surfactant obtained by the sequential reaction of ethylenediamine with propylene oxide and ethylene oxide in the presence of a catalyst, said surfactant containing polyoxypropylene chains having an average molecular weight of between about 750 and about 6,750 and polyoxyethylene chains constituting between about 10 and about 80 weight percent of said surfactant; 2. a substrate reagent formulation containing: a. sodium alpha-ketoglutarate; b. mannitol; and c. said nitrogen-containing polyoxyalkylene nonionic surfactant; the method comprising the steps of: i. dissolving said reagents in separate portions of water to produce liquid reagents; ii. mixing the liquid reagent containing said coenzyme reagent formulation with a biological specimen to form a specimen/reagent test system; iii. incubating said system; iv. thereafter adding the liquid reagent containing said substrate reagent formulation to said system; and v. measuring a property of said system at two different predetermined points in time following the addition of said liquid reagent containing said substrate reagent formulation.

Claim 29

The method as set forth in claim 19 wherein said property of the system measured is light absorbance.

Claim 30

The method as set forth in claim 19 wherein said nitrogen-containing surfactant is solid and the polyoxypropylene chains thereof have an average molecular weight of less than about 4,000.

Claim 33

a substrate reagent formulation containing: a. sodium alpha-ketoglutarate; b. mannitol; and c. said nitrogen-containing polyoxyalkylene nonionic surfactant; the method comprising the steps of: i. dissolving said reagents in separate portions of water to produce liquid reagents; ii. mixing the liquid reagent containing said coenzyme reagent formulation with a biological specimen to form a specimen/reagent test system; iii. incubating said system; iv. thereafter adding the liquid reagent containing said substrate reagent formulation to said system; and v. measuring a property of said system at two different predetermined points in time following the addition of said liquid reagent containing said substrate reagent formulation.

Claim 34

a substrate reagent formulation containing: a. sodium alpha-ketoglutarate; b. mannitol; and c. said nitrogen-containing polyoxyalkylene nonionic surfactant; the method comprising the steps of: i. dissolving said reagents in separate portions of water to produce liquid reagents; ii. mixing the liquid reagent containing said coenzyme reagent formulation with a biological specimen to form a specimen/reagent test system; iii. incubating said system; iv. thereafter adding the liquid reagent containing said substrate reagent formulation to said system; and v. measuring a property of said system at two different predetermined points in time following the addition of said liquid reagent containing said substrate reagent formulation.

Claim 35

The method as set forth in claim 22 wherein said property of the system measured is light absorbance.

Claim 36

The method as set forth in claim 22 wherein said nitrogen-containing surfactant is solid and the polyoxypropylene chains thereof have an average molecular weight of less than about 4,000.