Patent US3959080A successfully refreshed from Google Patents.
US3959080A
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Carrier matrix for the fixation of biochemically effective substances and process for the preparation thereof
Assignee: Merck Patent GmbH
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Filing: 1973-09-26
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Granted: 1976-05-25
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Expires: 1993-09-26
Abstract
Abstract Biochemically active compounds e.g., proteins and particulate biological materials such as bacteria and viruses, are immobilized on a water-swellable or water-insoluble matrix by covalent bonding to a partially crosslinked polymer containing reactive groups capable of binding the biochemically active compound thereto.
Inventors
- 👤 Hans Dieter Orth
- 👤 Wolfgang Brummer
- 👤 Michael Klockow
- 👤 Norbert Hennrich
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 09:26
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54353; A; A61; A61K; A61K47/00; A61K47/50; A61K47/51; A61K47/56; A61K47/58; A61K47/61; C; C08; C08F; C08F8/00; C12; C12N; C12N11/00; C12N11/02; C12N11/06; C12N11/10; C12N11/12; C12Q; C12Q1/00; C12Q1/34; C12Q1/37; Y; Y10; Y10S; Y10S530/00; Y10S530/81; Y10S530/812; Y10S530/813; Y10S530/814
Claims Analysis
Total Claims: 27
Claim 1
Independent Claim
107 words
A water-insoluble, partially covalently cross-linked carboxymethylcellulose hydrazide derivative having a swelling capacity, measured as water absorption per gram of dry material, of 10-80 ml. H 2 O/g., an average of about 150-2,000 glucose units per molecule and containing about 2-4.5 meq. of acid hydrazide groups per gram of dry material, wherein at least 10% of said hydrazide groups are cross-linked by --CO--NH--Z--Y--Z'--NH--CO-- groups wherein Y is a single covalent bond or a linear divalent alkylene chain of 1-18 carbon atoms interrupted by 0-4 oxygen atoms, Z and Z' are each a single covalent bond, --N = CH--, --NHCH 2 --, --NHCH 2 CHOH--, --NHCO--, --NH--CO--NH-- or --NH--CS--NH--.
Claim 2
Dependent Claim
107 words
A water-insoluble, partially covalently cross-linked carboxymethylcellulose hydrazide derivative having a swelling capacity, measured as water absorption per gram of dry material, of 10-80 ml. H 2 O/g., an average of about 150-2,000 glucose units per molecule and containing about 2-4.5 meq. of acid hydrazide groups per gram of dry material, wherein at least 10% of said hydrazide groups are cross-linked by --CO--NH--Z--Y--Z'--NH--CO-- groups wherein Y is a single covalent bond or a linear divalent alkylene chain of 1-18 carbon atoms interrupted by 0-4 oxygen atoms, Z and Z' are each a single covalent bond, --N = CH--, --NHCH 2 --, --NHCH 2 CHOH--, --NHCO--, --NH--CO--NH-- or --NH--CS--NH--.
Claim 3
Dependent Claim
13 words
A carboxymethylcellulose derivative according to claim 1, wherein Z and Z' are alike.
Claim 4
Dependent Claim
17 words
A carboxymethylcellulose derivative according to claim 2, wherein at least 70% of the hydrazide groups are uncross-linked.
Claim 5
Dependent Claim
15 words
A carboxymethylcellulose derivative according to claim 3, wherein 15-20% of the hydrazide groups are cross-linked.
Claim 6
Dependent Claim
14 words
A carboxymethylcellulose derivative according to claim 4, containing about 1,500 glucose units per molecule.
Claim 7
Dependent Claim
21 words
A carboxymethylcellulose derivative according to claim 1, wherein Y is divalent alkylene of 1-8 carbon atoms interrupted by 0-4 oxygen atoms.
Claim 8
Dependent Claim
16 words
A carboxymethylcellulose derivative according to claim 6, wherein Y is divalent alkylene of 3-6 carbon atoms.
Claim 9
Dependent Claim
13 words
A carboxymethylcellulose derivative according to claim 1, which is cross-linked by glutaric dialdehyde.
Claim 10
Dependent Claim
12 words
A carboxymethylcellulose derivative according to claim 1, which is cross-linked by 1,3-bis(2,3-epoxypropoxy)butane.
Claim 11
Dependent Claim
12 words
A carboxymethylcellulose derivative according to claim 1, which is cross-linked by epichlorohydrin.
Claim 12
Dependent Claim
34 words
A carboxymethylcellulose derivative according to claim 1, which is cross-linked by conversion of said hydrazide groups to azide groups and cross-linking of the resultant azide groups with an alkylenediamine to form an N-(6-aminoalkyl)-carbamoyl methylcellulose.
Claim 13
Dependent Claim
53 words
A carboxymethylcellulose derivative according to claim 1, wherein uncross-linked acid hydrazide groups are substituted by reactive groups comprising at least one member selected from the group consisting of azide, --CHO, --CH 2 Cl, --CH 2 Br, --CH 2 I, ##EQU7## --NH 2 , --COCl, --COBr, --COI, --NCO, --SCN, --COOH or diazonium salt groups.
Claim 14
Dependent Claim
15 words
A carboxymethylcellulose derivative according to claim 12, wherein said reactive groups are acid azide groups.
Claim 15
Dependent Claim
15 words
A carboxymethylcellulose derivative according to claim 12, wherein said reactive groups are free aldehyde groups.
Claim 16
Dependent Claim
14 words
A carboxymethylcellulose derivative according to claim 12, wherein said reactive groups are carboxyl groups.
Claim 17
Dependent Claim
15 words
A carboxymethylcellulose derivative according to claim 12, wherein said reactive groups are diazonium salt groups.
Claim 18
Dependent Claim
16 words
A carboxymethylcellulose derivative according to claim 12, which is partially cross-linked by glutaric dialdehyde or hexamethylenediamine.
Claim 19
Dependent Claim
33 words
A carboxymethylcellulose derivative according to claim 12, which is cross-linked by conversion of said hydrazide groups to a corresponding azide and cross-linking the resultant azide with an alkylenediamine to form an N-(6-aminoalkyl)-carbamoyl methylcellulose.
Claim 20
Dependent Claim
14 words
A carboxymethylcellulose derivative according to claim 18, wherein said N-(6-aminoalkyl)-carbamoyl methylcellulosoe is succinylated aminoalkylcellulose.
Claim 21
Dependent Claim
24 words
A biochemically active water-swellable or water-insoluble preparation comprising at least one biochemically active compound covalently bonded to the polymeric material according to claim 12.
Claim 22
Dependent Claim
16 words
A preparation according to claim 20, wherein said biochemically active compound is a protein or polypeptide.
Claim 23
Dependent Claim
17 words
A preparation according to claim 21, wherein said biochemically active compound is an enzyme or enzyme effector.
Claim 24
Dependent Claim
16 words
A preparation according to claim 22, wherein said biochemically active compound is a protease or peptidase.
Claim 25
Dependent Claim
48 words
A process for preparing a carboxymethylcellulose derivative according to claim 1, which comprises reacting an uncross-linked, water-soluble carboxymethylcellulose polymer containing cross-linkable acid hydrazide or azide groups with a bifunctional cross-linking agent reactive with said groups to covalently cross-link a portion of said groups and thereby form said derivative.
Claim 26
Dependent Claim
16 words
A process according to claim 24, wherein said bifunctional cross-linking agent is glutaric dialdehyde or hexamethylenediamine.
Claim 27
Dependent Claim
78 words
A process according to claim 24, further comprising substituting uncross-linked acid hydrazide groups by reactive groups capable of covalent bonding to an amino, hydroxyl, mercapto or phenyl group of a biochemically active compound, which reactive groups comprise at least one member selected from the group consisting of acid azide groups, --CHO, --CH 2 Hal, ##EQU8## --NH 2 , --COHal, --NCO, SCN, COOH or diazonium salt groups, in which Hal is Cl, Br or I, to form said derivative.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1979-05-25 | 1979-11-24 | 1980-05-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1983-05-25 | 1983-11-24 | 1984-05-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1987-05-25 | 1987-11-24 | 1988-05-25 | $7700.00 | Window Closed / Lapsed |