Patent US3981981A successfully refreshed from Google Patents.
US3981981A
Expired / Lapsed
US
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Method of radioimmunoassay employing diffusion
Assignee: Individual
•
Filing: 1974-01-18
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Granted: 1976-09-21
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Expires: 1994-01-18
Abstract
Abstract A method for the radioimmunoassay of small molecules consisting of bringing the large-molecule antibody of the small-molecule antigen to be determined into a slit-shaped chamber between dialysis membranes in a reaction vessel, bringing the antigen to be determined into this tank, into which a specified quantity of the radioactive form of this antigen has been added. During incubation the antigen and its radioactive form freely penetrate through the membranes and react with the antibody, whereupon washing stage is accomplished. Water and the unreacted antigen freely pass through the membranes, and the radioactivity of the washed complex remaining between the membranes is measured and the antigen is determined from this quantitatively in a way known.
Inventors
- 👤 Matti Antero Reunanen
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 05:00
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/536; G01N33/537; Y; Y10; Y10S; Y10S436/00; Y10S436/804; Y10S436/807; Y10S436/815; Y10S436/817
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
107 words
A method of radioimmunoassay comprising the steps of: a. bringing the large-molecule antibody of the small-molecule antigen to be determined into a slit-shaped chamber between dialysis membranes in a reaction vessel, b. bringing the antigen to be determined into said vessel, into which a specified quantity of the radioactive form of this antigen has been added, c. incubating, whereby the antigen and its radioactive form freely penetrate through the membranes and react with the antibody, d. washing, whereby water and the unreacted antigen freely pass through the membranes, and e. measuring the radioactivity of the washed complex remaining between the membranes, and f. determining the antigen quantitatively.
Claim 2
Independent Claim
107 words
A method of radioimmunoassay comprising the steps of: a. bringing the large-molecule antibody of the small-molecule antigen to be determined into a slit-shaped chamber between dialysis membranes in a reaction vessel, b. bringing the antigen to be determined into said vessel, into which a specified quantity of the radioactive form of this antigen has been added, c. incubating, whereby the antigen and its radioactive form freely penetrate through the membranes and react with the antibody, d. washing, whereby water and the unreacted antigen freely pass through the membranes, and e. measuring the radioactivity of the washed complex remaining between the membranes, and f. determining the antigen quantitatively.
Claim 3
Dependent Claim
24 words
A method according to claim 1 wherein the antibody is brought into its slit-shaped chamber between the membranes as absorbed into a porous sheet.
Claim 4
Dependent Claim
22 words
A method according to claim 1 wherein a number of vessels are contained in a cassette during the incubation and washing steps.
Claim 5
Dependent Claim
32 words
A method according to claim 3 wherein the agitation of liquid in the tank necessary during incubation and washing is produced by keeping the cassette with its contents on a swaying base.
Claim 6
Dependent Claim
34 words
A method according to claim 1 wherein in said vessel lies on its side during washing and the washing liquid can be changed by lowering and raising the liquid level in the surrounding container.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1979-09-21 | 1980-03-22 | 1980-09-21 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1983-09-21 | 1984-03-22 | 1984-09-21 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1987-09-21 | 1988-03-22 | 1988-09-21 | $7700.00 | Window Closed / Lapsed |