Patent US4080263A successfully refreshed from Google Patents.
US4080263A
Expired / Lapsed
US
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Process and reagent for the rapid quantitative determination of lactate or alanine
Assignee: Roche Diagnostics GmbH
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Filing: 1976-08-11
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Granted: 1978-03-21
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Expires: 1996-08-11
Abstract
Abstract L-lactate is quantitatively determined by the reaction thereof with nicotinamide-adenine-dinucleotide (NAD) in the presence of lactate dehydrogenase (LDH) with the formation of reduced NAD (NADH) and pyruvate, reaction of the latter with glutamate in the presence of glutamate-pyruvate-transaminase (GPT) with the formation of alanine and α-ketoglutarate in alkaline buffered solution and measurement of the NADH formed, wherein the reaction is carried out in the presence of at least 0.4 mol/liter of carbonate or bicarbonate.
Inventors
- 👤 Erich Bernt
- 👤 Peter Scheibe
- 👤 Ingeborg Gutmann
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 05:29
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/48; C12Q1/52; C12Q1/26; C12Q1/32
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
81 words
In a process for the quantitative determination of L-lactate by reaction with nicotinamide-adenine-dinucleotide in the presence of lactate dehydrogenase with the formation of reduced nicotinamide-adenine-dinucleotide and pyruvate, reaction of the latter with glutamate in the presence of glutamatepyruvate-transaminase with the formation of alanine and α-ketoglutarate in alkaline buffered solution and measurement of the reduced nicotinamide-adenine-dinucleotide formed, the improvement comprising carrying out the said reaction in the presence of at least 0.4 mol of carbonate or bicarbonate per liter of buffered solution.
Claim 2
Dependent Claim
81 words
In a process for the quantitative determination of L-lactate by reaction with nicotinamide-adenine-dinucleotide in the presence of lactate dehydrogenase with the formation of reduced nicotinamide-adenine-dinucleotide and pyruvate, reaction of the latter with glutamate in the presence of glutamatepyruvate-transaminase with the formation of alanine and α-ketoglutarate in alkaline buffered solution and measurement of the reduced nicotinamide-adenine-dinucleotide formed, the improvement comprising carrying out the said reaction in the presence of at least 0.4 mol of carbonate or bicarbonate per liter of buffered solution.
Claim 3
Dependent Claim
24 words
Process as claimed in claim 1, wherein the reaction is carried out in a solution which contains 0.45 to 1.0 mol/liter of carbonate buffer.
Claim 4
Dependent Claim
20 words
Process as claimed in claim 1, wherein the reaction is carried out at a pH of from 9.5 to 10.5.
Claim 5
Dependent Claim
24 words
Process as claimed in claim 1, wherein said reaction solution also contains 2 to 30 mMol alkali metal azide per liter of buffered solution.
Claim 6
Dependent Claim
78 words
In a process for the quantitative determination of L-alanine comprising reacting a sample containing L-alanine with a solution composed of an alkaline buffer, reduced nicotinamide-adenine-dinucleotide, oxoglutarate and lactate dehydrogenase; measuring the absorbtion of the solution, reacting the solution with glutamate-pyruvate-transaminase, measuring the absorbtion of the solution, then determining the alannine concentration from the difference in the absorbtions, the improvement comprising carrying out the reactions in the presence of at least 0.4 mol of carbonate or bicarbonate per liter.
Claim 7
Dependent Claim
78 words
In a process for the quantitative determination of L-alanine comprising reacting a sample containing L-alanine with a solution composed of an alkaline buffer, reduced nicotinamide-adenine-dinucleotide, oxoglutarate and lactate dehydrogenase; measuring the absorbtion of the solution, reacting the solution with glutamate-pyruvate-transaminase, measuring the absorbtion of the solution, then determining the alannine concentration from the difference in the absorbtions, the improvement comprising carrying out the reactions in the presence of at least 0.4 mol of carbonate or bicarbonate per liter.
Claim 8
Dependent Claim
32 words
Reagent for the quantitative determination of L-lactate, which comprises lactate-dehydrogenase, glutamate-pyruvate-transaminase, nicotinamide-adenine-dinucleotide and glutamate and is characterized by a content of 0.40 to 1.2 mol/liter carbonate buffer of pH 9.5 to 10.5.
Claim 9
Dependent Claim
32 words
Reagent for the quantitative determination of L-lactate, which comprises lactate-dehydrogenase, glutamate-pyruvate-transaminase, nicotinamide-adenine-dinucleotide and glutamate and is characterized by a content of 0.40 to 1.2 mol/liter carbonate buffer of pH 9.5 to 10.5.
Claim 10
Dependent Claim
51 words
Reagent as claimed in claim 6, which comprises 0.45 to 1.0 mol/liter carbonate buffer of pH 9.5 to 10.5, as well as 50 - 150 mMol/liter glutamate, 3 - 10 m/Mol/liter nicotinamide-adenine-dinucleotide and 20 to 60 U lactate dehydrogenase and 2 to 4 U glutamate-pyruvate transaminase per ml. of buffer solution.
Claim 11
Dependent Claim
21 words
Reagent as claimed in claim 6, which additionally contains 2 to 30 mMol alkali metal azide per liter of buffer solution.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-03-20 | 1981-09-19 | 1982-03-21 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-03-20 | 1985-09-19 | 1986-03-21 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-03-20 | 1989-09-19 | 1990-03-21 | $7700.00 | Window Closed / Lapsed |