Patent US4152214A successfully refreshed from Google Patents.
US4152214A
Expired / Lapsed
US
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Production of homodeoxyharringtonine and other cephalotaxine esters by tissue culture
Assignee: United States Department of Agriculture
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Filing: 1977-10-07
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Granted: 1979-05-01
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Expires: 1997-10-07
Abstract
Abstract Tissue of Cephalotaxus harringtonia has been successfully cultured and cephalotaxine and its chemotherapeutically active alkaloid esters have been recovered from the resultant callus and the culture medium. A novel alkaloid compound, homodeoxyharringtonine, has also been discovered in the culture medium.
Inventors
- 👤 Norman E. Delfel
- 👤 John A. Rothfus
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 18:55
CPC Classifications
C; C07; C07D; C07D491/00; C07D491/12; C07D491/20
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
82 words
A method of producing alkaloid compounds selected from the group consisting of cephalotaxine, harringtonine, isoharringtonine, homoharringtonine, deoxyharringtonine and homodeoxyharringtonine from Cephalotaxus harringtonia callus comprising the steps of: a. providing living tissue of Cephalotaxus harringtonia; b. providing a nutrient culture medium suitable for callus formation from said tissue; c. culturing said tissue on said medium to produce callus from said tissue whereby said callus actively secretes said alkaloid compounds into said medium; and d. recovering said actively secreted alkaloid compounds from said medium.
Claim 2
Independent Claim
82 words
A method of producing alkaloid compounds selected from the group consisting of cephalotaxine, harringtonine, isoharringtonine, homoharringtonine, deoxyharringtonine and homodeoxyharringtonine from Cephalotaxus harringtonia callus comprising the steps of: a. providing living tissue of Cephalotaxus harringtonia; b. providing a nutrient culture medium suitable for callus formation from said tissue; c. culturing said tissue on said medium to produce callus from said tissue whereby said callus actively secretes said alkaloid compounds into said medium; and d. recovering said actively secreted alkaloid compounds from said medium.
Claim 3
Dependent Claim
17 words
The method as described in claim 1 wherein said living tissue in step (a) is leaf tissue.
Claim 4
Dependent Claim
31 words
The method as described in claim 1 wherein said culture medium is an aqueous solution and is absorbed into a bridge of absorbent material at least partially immersed in said solution.
Claim 5
Dependent Claim
22 words
The method as described in claim 1 wherein said actively secreted alkaloid compound recovered from said medium in step (d) is homodeoxyharringtonine.
References Cited (Backward Citations)
US3628287A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1982-04-30 | 1982-10-30 | 1983-05-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1986-04-30 | 1986-10-30 | 1987-05-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1990-04-30 | 1990-10-30 | 1991-05-01 | $7700.00 | Window Closed / Lapsed |