Patent US4153513A successfully refreshed from Google Patents.
US4153513A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method and apparatus for the continuous determination of the concentration of an enzyme substrate
Assignee: Roche Diagnostics GmbH
•
Filing: 1977-08-19
•
Granted: 1979-05-08
•
Expires: 1997-08-19
Abstract
Abstract Enzyme substrates in aqueous liquids are continuously measured by introducing a sample containing the substrate to be determined into a current of buffer solution, reacting the substrate on an immobilized enzyme and measuring, in a measurement chamber, a physical change in the solution brought about by the reaction, wherein a current of buffer solution containing the sample to be determined is passed alternatingly (i) directly through the measurement chamber with the production of a reference signal and (ii) first over the immobilized enzyme and thereafter through the measurement chamber with the production of a measurement signal, and relating the reference signal to the measurement signal to indicate the enzyme substrate initially present.
Inventors
- 👤 Hermann Edelmann
- 👤 Peter Kraft
- 👤 Karlheinz Mann
- 👤 Peter Schuler
- 👤 Alexander Hagen
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 14:44
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/28; G01N27/30; G01N27/327; G01N27/3271
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
121 words
Method for the continuous determination of the concentration of an enzyme substrate in an aqueous liquid which comprises introducing a sample containing the substrate to be determined into a current of flowing buffer solution, reacting the substrate contained in said buffer solution with an immobilized enzyme and measuring a physical change in the solution brought about by said reaction using a single measurement chamber, by passing the buffer solution containing the sample to be determined in an alternating manner (i) directly through the measurement chamber thereby producing a first measurement signal and (ii) then over said immobilized enzyme and thereafter through said measurement chamber thereby producing a second measurement signal and comparing the signals to determine the concentration of the substrate.
Claim 2
Dependent Claim
121 words
Method for the continuous determination of the concentration of an enzyme substrate in an aqueous liquid which comprises introducing a sample containing the substrate to be determined into a current of flowing buffer solution, reacting the substrate contained in said buffer solution with an immobilized enzyme and measuring a physical change in the solution brought about by said reaction using a single measurement chamber, by passing the buffer solution containing the sample to be determined in an alternating manner (i) directly through the measurement chamber thereby producing a first measurement signal and (ii) then over said immobilized enzyme and thereafter through said measurement chamber thereby producing a second measurement signal and comparing the signals to determine the concentration of the substrate.
Claim 3
Dependent Claim
26 words
A method as claimed in claim 1 wherein the sample containing the substrate to be determined is introduced continuously into the current of flowing buffer solution.
Claim 4
Dependent Claim
24 words
Method as claimed in claim 1 wherein the sample containing the substrate to be determined is dialyzed into the current of flowing buffer solution.
Claim 5
Dependent Claim
22 words
Method as claimed in claim 1 wherein the sample to be determined is introduced directly into the current of flowing buffer solution.
Claim 6
Dependent Claim
47 words
Method as claimed in claim 1 wherein the current of flowing buffer solution containing the sample is allowed to flow through said measurement chamber until a constant base signal or measurement signal is obtained and thereafter said solution is passed to the alternate route (i) or (ii).
Claim 7
Dependent Claim
29 words
Method as claimed in claim 1 wherein interfering components of the sample contained in the current of flowing buffer solution are changed to render them non-interfering or removed therefrom.
Claim 8
Dependent Claim
37 words
Method as claimed in claim 6 wherein the current of flowing buffer solution containing the sample, after passing the measurement chamber, is contacted with an immobilized enzyme until the substrate contained in said solution is completely reacted.
Claim 9
Dependent Claim
43 words
Method as claimed in claim 6 wherein the current of flowing buffer solution containing the sample is contacted with an adsorption agent, or an ion exchanger, for the removal of any interfering components from the current of flowing buffer solution containing the sample.
Claim 10
Dependent Claim
29 words
Method as claimed in claim 4 wherein the sample to be determined is prediluted with a buffer solution and is then introduced into the current of flowing buffer solution.
Claim 11
Dependent Claim
32 words
Method as claimed in claim 1 wherein said sample is a protein containing sample and is first diluted with a heparin solution before being introduced into the current of flowing buffer solution.
Claim 12
Dependent Claim
19 words
Method as claimed in claim 10 wherein said diluted solution is dialyzed into the current of flowing buffer solution.
Claim 13
Dependent Claim
99 words
Apparatus for conducting the method as claimed in claim 1 which apparatus comprises a buffer storage container communicating with a sample introduction device, a sample inlet stream also communicating with said sample introduction device, said device being capable of mixing the sample and buffer and communicating with a bridging channel to a chamber holding immobilized enzyme and with a bridging channel to a measurement chamber, and a changeover valve therein adapted to alternatingly open and close said bridging channels to permit alternating communication between said sample introduction device and (i) the immobilized enzyme chamber and (ii) the measurement chamber.
Claim 14
Dependent Claim
117 words
Apparatus as claimed in claim 12 wherein the changeover valve comprises three channels in which, in a first position of the changeover valve, the first channel forms the bridging tube, the second channel passes the flow from the measurement chamber to the immobilized enzyme and the third channel connects the flow from the immobilized enzyme with a pipe leading to the buffer storage container and, in a second position of the changeover valve, the first channel forms the pipe to the immobilized enzyme, the second channel connects the flow from the immobilized enzyme with the measurement chamber and the third channel connects the flow from the measurement chamber with the pipe leading to the buffer storage container.
Claim 15
Dependent Claim
102 words
Apparatus as claimed in claim 12 wherein the changeover valve has two channels, the first of which contains an immobilized enzyme, in which, in a first position of the changeover valve, the first channel connects the flow from the measurement chamber with a pipe leading to the buffer storage container and the second channel forms the bridging pipe and, in a second position of the changeover valve, the first channel connects the flow from the same introduction device with the measurement chamber and the second channel connects the flow from the measurement chamber with the pipe leading to the buffer storage container.
Claim 16
Dependent Claim
28 words
Apparatus as claimed in claim 12 additionally comprising a reactor for the removal or conversion of interfering components arranged in the conduit leading to the buffer storage container.
Claim 17
Dependent Claim
19 words
Apparatus as claimed in claim 15 wherein said reactor contains the same immobilized enzyme as said immobilized enzyme chamber.
Claim 18
Dependent Claim
43 words
Apparatus as claimed in claim 12 additionally comprising an injection device and wherein the measurement chamber generates a measurement signal which controls the introduction of a certain amount of an injection liquid into the liquid in which the enzyme substrate concentration is determined.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1982-05-07 | 1982-11-06 | 1983-05-08 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1986-05-07 | 1986-11-06 | 1987-05-08 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1990-05-07 | 1990-11-06 | 1991-05-08 | $7700.00 | Window Closed / Lapsed |