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US4158546A
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Composition, test device and method for determining the presence of urobilinogen in a test sample
Assignee: Bayer Corp
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Filing: 1978-07-24
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Granted: 1979-06-19
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Expires: 1998-07-24
Abstract
Abstract A novel test composition, device and method for determining the presence of urobilinogen in a test sample. The composition is capable of producing a detectable response, such as a change in color or change in light absorption, thus indicating the presence of urobilinogen, both on a qualitative and a semi-quantitative basis. Briefly, the composition comprises para-di(lower alkyl)aminobenzaldehyde as an indicator compound, a buffer substance capable of producing a pH of about 0.5 to 3, and a compound having the structure <IMAGE> in which X is O or S, and in which R and R', same or different, are lower alkyl, or R and R' together are lower alkylene or <IMAGE> wherein n is an integer of 1 to about 6. The device comprises a carrier matrix incorporated with the composition. The method of use comprises contacting a test sample with the composition or device and observing any detectable response formed therein.
Inventors
- 👤 Charles T. W. Lam
- 👤 Chauncey O. Rupe
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 10:33
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/72; Y; Y10; Y10T; Y10T436/00; Y10T436/14; Y10T436/145555; Y10T436/146666
Claims Analysis
Total Claims: 26
Claim 1
Independent Claim
94 words
In a composition capable of producing a detectable response in the presence of urobilinogen in a test sample, which composition comprises p-di(lowr alkyl)aminobenzaldehyde as an indicator and a buffer substance capable of producing a pH of about 0.5 to 3, the improvement wherein said composition further comprises a compound having the structure ##STR7## in which X is O or S; and in which R and R', same or different, are lower alkyl, or in which R and R' together are lower alkylene or ##STR8## wherein n is an integer from 1 to about 6.
Claim 2
Dependent Claim
94 words
In a composition capable of producing a detectable response in the presence of urobilinogen in a test sample, which composition comprises p-di(lowr alkyl)aminobenzaldehyde as an indicator and a buffer substance capable of producing a pH of about 0.5 to 3, the improvement wherein said composition further comprises a compound having the structure ##STR7## in which X is O or S; and in which R and R', same or different, are lower alkyl, or in which R and R' together are lower alkylene or ##STR8## wherein n is an integer from 1 to about 6.
Claim 3
Dependent Claim
28 words
The improvement of claim 1 in which R and R' together form --CH 2 ) n , in which n is an integer of 1 to about 6.
Claim 4
Dependent Claim
11 words
The improvement of claim 1 in which said compound is 2-imidazolidone.
Claim 5
Dependent Claim
12 words
The improvement of claim 3 in which said indicator compound is p-dimethylaminobenzaldehyde.
Claim 6
Dependent Claim
12 words
The improvement of claim 1 in which said compound is malonyl urea.
Claim 7
Dependent Claim
11 words
The improvement of claim 5 in which said indicator is p-diethylaminobenzaldehyde.
Claim 8
Dependent Claim
25 words
A composition for determining the presence of urobilinogen in a test sample, the composition comprising 2-imidazolidone, p-dimethylaminobenzaldehyde, sulfosalicylic acid, triethanolamine borate, caffeine, and ascorbic acid.
Claim 9
Dependent Claim
25 words
A composition for determining the presence of urobilinogen in a test sample, the composition comprising 2-imidazolidone, p-dimethylaminobenzaldehyde, sulfosalicylic acid, triethanolamine borate, caffeine, and ascorbic acid.
Claim 10
Dependent Claim
26 words
A composition for determining the presence of urobilinogen in a test sample, the composition comprising malonyl urea, p-diethylaminobenzaldehyde, sulfosalicylic acid, caffeine, triethanolamine borate and ascorbic acid.
Claim 11
Dependent Claim
26 words
A composition for determining the presence of urobilinogen in a test sample, the composition comprising malonyl urea, p-diethylaminobenzaldehyde, sulfosalicylic acid, caffeine, triethanolamine borate and ascorbic acid.
Claim 12
Independent Claim
25 words
A device for determining the presence of urobilinogen in a test sample comprising carrier matrix incorporated with the test composition of any of claims 1-8.
Claim 13
Independent Claim
31 words
A method for determining the presence of urobilinogen in a test sample, said method comprising contacting said sample with the composition of any of claims 1-8 and observing any detectable response.
Claim 14
Dependent Claim
29 words
A method for determining the presence of urobilinogen in a test sample, said method comprising contacting said sample with the device of claim 9 and observing any detectable response.
Claim 15
Independent Claim
30 words
A method for preparing a test device for determining the presence of urobilinogen in a test sample comprising incorporating a carrier matrix with the composition of any of claims 1-8.
Claim 16
Dependent Claim
112 words
A one-dip method for preparing a test device for determining the presence of urobilinogen in a test sample, the method comprising the steps of preparing a solution by dissolving in a suitable solvent p-di(lower alkyl)aminobenzaldehyde as an indicator, a buffer substance capable of providing a pH of about 0.5 to 3, and a compound having the structure ##STR9## in which X is O or S; and in which R and R', same or different, are lower alkyl, or in which R and R' together are lower alkylene or ##STR10## in which n is an integer from 1 to about 6; contacting a carrier matrix with said solution; and drying said carrier matrix.
Claim 17
Dependent Claim
112 words
A one-dip method for preparing a test device for determining the presence of urobilinogen in a test sample, the method comprising the steps of preparing a solution by dissolving in a suitable solvent p-di(lower alkyl)aminobenzaldehyde as an indicator, a buffer substance capable of providing a pH of about 0.5 to 3, and a compound having the structure ##STR9## in which X is O or S; and in which R and R', same or different, are lower alkyl, or in which R and R' together are lower alkylene or ##STR10## in which n is an integer from 1 to about 6; contacting a carrier matrix with said solution; and drying said carrier matrix.
Claim 18
Dependent Claim
29 words
The method of claim 13 wherein said compound is one in which R and R' together form --CH.sub.2).sub.n in which n is an integer from 1 to about 6.
Claim 19
Dependent Claim
10 words
The method of claim 13 wherein said compound is 2-imidazolidone.
Claim 20
Dependent Claim
13 words
The method of claim 13, 14, or 15 wherein said indicator is p-dimethylaminobenzaldehyde.
Claim 21
Dependent Claim
11 words
The method of claim 13 wherein said compound is malonyl urea.
Claim 22
Dependent Claim
10 words
The method of claim 17 wherein said indicator is p-diethylaminobenzaldehyde.
Claim 23
Dependent Claim
70 words
A one-dip method for preparing a test device for determining the presence of urobilinogen in a test sample, said method comprising the steps of preparing an impregnating solution by dissolving in a suitable solvent, sulfosalicylic acid, p-dimethylaminobenzaldehyde, triethanolamine borate, 2-imidazolidone, caffeine and ascorbic acid; impregnating a carrier matrix with said solution by immersing said matrix in said solution; and then, after removing it from said solution, drying said carrier matrix.
Claim 24
Dependent Claim
70 words
A one-dip method for preparing a test device for determining the presence of urobilinogen in a test sample, said method comprising the steps of preparing an impregnating solution by dissolving in a suitable solvent, sulfosalicylic acid, p-dimethylaminobenzaldehyde, triethanolamine borate, 2-imidazolidone, caffeine and ascorbic acid; impregnating a carrier matrix with said solution by immersing said matrix in said solution; and then, after removing it from said solution, drying said carrier matrix.
Claim 25
Dependent Claim
64 words
A one-dip method for preparing a test device for determining the presence of urobilinogen in a test sample, said method comprising the sequential steps of preparing a solution by dissolving in a suitable solvent sulfosalicylic acid, caffeine, ascorbic acid, triethanolamine borate, p-diethylaminobenzaldehyde, and malonyl urea; impregnating a carrier matrix with said solution by immersing said matrix in said solution; and drying said carrier matrix.
Claim 26
Dependent Claim
64 words
A one-dip method for preparing a test device for determining the presence of urobilinogen in a test sample, said method comprising the sequential steps of preparing a solution by dissolving in a suitable solvent sulfosalicylic acid, caffeine, ascorbic acid, triethanolamine borate, p-diethylaminobenzaldehyde, and malonyl urea; impregnating a carrier matrix with said solution by immersing said matrix in said solution; and drying said carrier matrix.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1982-06-18 | 1982-12-18 | 1983-06-19 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1986-06-18 | 1986-12-18 | 1987-06-19 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1990-06-18 | 1990-12-18 | 1991-06-19 | $7700.00 | Window Closed / Lapsed |