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US4166104A
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US
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Specific binding assay method and test kit employing polystyrene as separating agent
Assignee: Biodex Biotechnological and Diagnostic Systems Ltd
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Filing: 1977-11-16
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Granted: 1979-08-28
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Expires: 1997-11-16
Abstract
Abstract A specific binding assay method and test kit for determining a ligand, particularly an antigen or hapten, in, or the ligand binding capacity of, a liquid medium, particularly a body fluid such as serum, wherein the liquid medium is combined with assay reagent means including a labeled binding component, such as a radiolabeled form of the ligand, with the improvement that separation of the resulting bound-species and free-species of the labeled component is accomplished by selectively adsorbing the free-species onto a solid comprising a nonion-exchange cross-linked styrene polymer. The method is particularly suited to the radioimmunoassaydetection of nonproteinaceous haptens such as thyroxine, triiodothyronine, estriol, vitamin B 12 and digoxin. The styrene polymer adsorbent provides a very effective and readily available separation means for use in specific binding assays.
Inventors
- 👤 Daniel B. Wagner
- 👤 Zvi Gross
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 18:58
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/536; G01N33/537; G01N33/538; Y; Y10; Y10S; Y10S436/00; Y10S436/804; Y10S436/815; Y10S436/817
Claims Analysis
Total Claims: 37
Claim 1
Independent Claim
116 words
In a specific binding assay method for determining a ligand in or the ligand binding capacity of a liquid medium, wherein said liquid medium is combined with assay reagent means including a binding component incorporated with a label to form a binding reaction system having a bound-species and a free-species of said labeled component, wherein said bound-species and said free-species of said labeled component are separated, and wherein said label is measured in one of said separated bound-species and free-species of the labeled component, the improvement which comprises accomplishing said separation by selectively adsorbing said free-species of the labeled component onto a solid comprising a nonion-exchange cross-linked styrene polymer, said styrene polymer being a styrene-divinylbenzene copolymer.
Claim 2
Dependent Claim
116 words
In a specific binding assay method for determining a ligand in or the ligand binding capacity of a liquid medium, wherein said liquid medium is combined with assay reagent means including a binding component incorporated with a label to form a binding reaction system having a bound-species and a free-species of said labeled component, wherein said bound-species and said free-species of said labeled component are separated, and wherein said label is measured in one of said separated bound-species and free-species of the labeled component, the improvement which comprises accomplishing said separation by selectively adsorbing said free-species of the labeled component onto a solid comprising a nonion-exchange cross-linked styrene polymer, said styrene polymer being a styrene-divinylbenzene copolymer.
Claim 3
Dependent Claim
12 words
The method of claim 1 wherein said ligand is a nonproteinaceous hapten.
Claim 4
Dependent Claim
43 words
The method of claim 2 for determining said hapten in an aqueous liquid wherein said assay reagent means includes (i) as labeled component, said hapten or a binding alalog thereof incorporated with a label and (ii) a specific binding agent for said hapten.
Claim 5
Dependent Claim
10 words
The method of claim 3 wherein said hapten is thyroxine.
Claim 6
Dependent Claim
10 words
The method of claim 3 wherein said hapten is triiodothyronine.
Claim 7
Dependent Claim
10 words
The method of claim 3 wherein said hapten is estriol.
Claim 8
Dependent Claim
13 words
The method of claim 3 wherein said hapten is vitamin B 12 .
Claim 9
Dependent Claim
10 words
The method of claim 3 wherein said hapten is digoxin.
Claim 10
Dependent Claim
16 words
The method of claim 3 wherein said specific binding agent is an antibody for said hapten.
Claim 11
Dependent Claim
12 words
The method of claim 3 wherein said label is a radioactive atom.
Claim 12
Dependent Claim
37 words
The method of claim 2 for determining the binding capacity of an aqueous liquid for said hapten wherein said assay reagent means includes, as labeled component, said hapten or a binding analog thereof incorporated with a label.
Claim 13
Dependent Claim
10 words
The method of claim 11 wherein said hapten is triiodothyronine.
Claim 14
Dependent Claim
12 words
The method of claim 11 wherein said label is a radioactive atom.
Claim 15
Dependent Claim
19 words
The method of claim 1 wherein said assay reagent means includes a specific binding agent for said labeled component.
Claim 16
Dependent Claim
15 words
The method of claim 14 wherein said binding agent is an antibody for said ligand.
Claim 17
Dependent Claim
14 words
The method of claim 1 wherein said styrene polymer is in a particulate form.
Claim 18
Dependent Claim
31 words
The method of claim 16 wherein said separation is accomplished by adding said particulate form of said styrene polymer to said reaction mixture and thereafter separating such by centrifugation or filtration.
Claim 19
Dependent Claim
32 words
The method of claim 16 wherein said particulate form of said styrene polymer is contained in a column and wherein said separation is accomplished by passing said reaction mixture through said column.
Claim 20
Dependent Claim
35 words
The method of claim 1 wherein said styrene polymer is incorporated with a carrier matrix and wherein said separation is accomplished by contacting said carrier matrix with said reaction mixture and thereafter removing same therefrom.
Claim 21
Dependent Claim
58 words
A test kit for determining a ligand in a liquid medium, comprising, in package combination, (1) a container of said ligand or a binding analog thereof incorporated with a label, (2) a container of a binding agent for said ligand, and (3) a solid adsorbent comprising a nonion-exchange crosslinked styrene polymer, said styrene polymer being a styrenedivinylbenzene copolymer.
Claim 22
Dependent Claim
58 words
A test kit for determining a ligand in a liquid medium, comprising, in package combination, (1) a container of said ligand or a binding analog thereof incorporated with a label, (2) a container of a binding agent for said ligand, and (3) a solid adsorbent comprising a nonion-exchange crosslinked styrene polymer, said styrene polymer being a styrenedivinylbenzene copolymer.
Claim 23
Dependent Claim
13 words
The test kit of claim 20 wherein said ligand is a nonproteinaceous hapten.
Claim 24
Dependent Claim
18 words
The test kit of claim 20 wherein said hapten is thyroxine, triiodothyronine, estriol, vitamin B 12 or digoxin.
Claim 25
Dependent Claim
14 words
The test kit of claim 20 wherein said specific binding agent is an antibody.
Claim 26
Dependent Claim
13 words
The test kit of claim 20 wherein said label is a radioactive atom.
Claim 27
Dependent Claim
15 words
The test kit of claim 20 wherein said styrene polymer is in a particulate form.
Claim 28
Dependent Claim
19 words
The test kit of claim 25 wherein said particulate form of said styrene polymer is contained in a column.
Claim 29
Dependent Claim
16 words
The test kit of claim 20 wherein said styrene polymer is incorporated with a carrier matrix.
Claim 30
Dependent Claim
50 words
A test kit for determining the ligand binding capacity of a liquid medium, comprising, in package combination, (1) a container of said ligand or a binding analog thereof incorporated with a label, and (2) a solid adsorbent comprising a nonion-exchange crosslinked styrene polymer, said styrene polymer being a styrenedivinylbenzene copolymer.
Claim 31
Dependent Claim
50 words
A test kit for determining the ligand binding capacity of a liquid medium, comprising, in package combination, (1) a container of said ligand or a binding analog thereof incorporated with a label, and (2) a solid adsorbent comprising a nonion-exchange crosslinked styrene polymer, said styrene polymer being a styrenedivinylbenzene copolymer.
Claim 32
Dependent Claim
13 words
The test kit of claim 28 wherein said ligand is a nonproteinaceous hapten.
Claim 33
Dependent Claim
18 words
The test kit of claim 28 wherein said ligand binding capacity is the capacity for binding for triiodothyronine.
Claim 34
Dependent Claim
13 words
The test kit of claim 28 wherein said label is a radioactive atom.
Claim 35
Dependent Claim
15 words
The test kit of claim 28 wherein said styrene polymer is in a particulate form.
Claim 36
Dependent Claim
19 words
The test kit of claim 32 wherein said particulate form of said styrene polymer is contained in a column.
Claim 37
Dependent Claim
16 words
The test kit of claim 28 wherein said styrene polymer is incorporated with a carrier matrix.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1982-08-27 | 1983-02-26 | 1983-08-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1986-08-27 | 1987-02-26 | 1987-08-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1990-08-27 | 1991-02-26 | 1991-08-28 | $7700.00 | Window Closed / Lapsed |