Patent US4168262A successfully refreshed from Google Patents.
US4168262A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Anhydride modified microbial protein having reduced nucleic acid levels
Assignee: Cornell Research Foundation Inc
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Filing: 1978-03-13
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Granted: 1979-09-18
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Expires: 1998-03-13
Abstract
Abstract The process of the invention comprises disrupting microbial protein containing cells; derivatizing the resultant mixture comprising protein and nucleic acid with an acid anhydride; and then isoelectrically precipitating a nucleic acid diminished protein containing fraction from a nucleic acid enriched supernatant.
Inventors
- 👤 John E. Kinsella
- 👤 Jayarama K. Shetty
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 16:42
CPC Classifications
A; A23; A23J; A23J1/00; A23J1/008; A23J1/18; A23J3/00; A23J3/20; Y; Y10; Y10S; Y10S530/00; Y10S530/82; Y10S530/821; Y10S530/823; Y10S530/824
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
63 words
A method of reducing nucleic acid in microbially derived protein which comprises: (a) disrupting microbial cells to provide a mixture comprising protein and nucleic acid, (b) derivatizing an aqueous mixture comprising said protein and nucleic acid with an organic acid anhydride at a pH of above about 7.5, and then (c) reducing the pH to isoelectically precipitate a nucleic acid diminished protein concentrate.
Claim 2
Independent Claim
63 words
A method of reducing nucleic acid in microbially derived protein which comprises: (a) disrupting microbial cells to provide a mixture comprising protein and nucleic acid, (b) derivatizing an aqueous mixture comprising said protein and nucleic acid with an organic acid anhydride at a pH of above about 7.5, and then (c) reducing the pH to isoelectically precipitate a nucleic acid diminished protein concentrate.
Claim 3
Dependent Claim
23 words
A method as in claim 1 where the protein-nucleic acid mixture is derived from Candida utilis, Saccharomyces cerevisiae, Saccharomyces fragilis, or Saccharomyces carlsbergensis.
Claim 4
Independent Claim
20 words
A method as in claims 1 or 2 where the anhydride is succinic anhydride, dimethyl maleic anhydride or maleic anhydride.
Claim 5
Dependent Claim
12 words
A method as in claim 3 where the anhydride is succinic anhydride.
Claim 6
Independent Claim
15 words
A method as in claims 1 or 2 where the anhydride is a cyclic anhydride.
Claim 7
Dependent Claim
12 words
A method as in claim 1 where the anhydride is citraconic anhydride.
Claim 8
Independent Claim
26 words
A method as in claims 1, 2 or 6 where the isolectric precipitation of the protein concentrate is conducted at a pH of 4.2 to 4.5.
Claim 9
Dependent Claim
65 words
In a method of forming a nucleic acid diminished protein concentrate from a mixture of protein and nucleic acid obtained by disrupting microbial cells, the steps comprising: (a) derivatizing an aqueous mixture comprising the protein nucleic acid mixture with a cyclic organic anhydride at a pH of above about 7.5, and then (b) reducing the pH to isoelectically precipitate a nucleic acid diminished protein concentrate.
Claim 10
Dependent Claim
65 words
In a method of forming a nucleic acid diminished protein concentrate from a mixture of protein and nucleic acid obtained by disrupting microbial cells, the steps comprising: (a) derivatizing an aqueous mixture comprising the protein nucleic acid mixture with a cyclic organic anhydride at a pH of above about 7.5, and then (b) reducing the pH to isoelectically precipitate a nucleic acid diminished protein concentrate.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1982-09-17 | 1983-03-19 | 1983-09-18 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1986-09-17 | 1987-03-19 | 1987-09-18 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1990-09-17 | 1991-03-19 | 1991-09-18 | $7700.00 | Window Closed / Lapsed |