Patent US4169014A successfully refreshed from Google Patents.
US4169014A
Expired / Lapsed
US
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Method of immobilizing proteinaceous substances
Assignee: FMC Corp
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Filing: 1978-04-03
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Granted: 1979-09-25
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Expires: 1998-04-03
Abstract
Abstract Proteins such as enzymes are immobilized on a microporous member comprising a binder or matrix and finely divided filler particles dispersed throughout the binder. The proteins are coupled to the filler particles, and the microporous member has a relatively large surface area and a large number of available protein coupling sites. Enzymes coupled to the microporous member have a relatively high reaction efficiency when used to act on a substrate.
Inventors
- 👤 Bruce S. Goldberg
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 23:26
CPC Classifications
C; C12; C12P; C12P19/00; C12P19/24; C12M; C12M21/00; C12M21/18; C12M25/00; C12M25/16; C12N; C12N11/00; C12N11/02; C12N11/04; C12N11/08; C12N11/089; Y; Y10; Y10S; Y10S530/00; Y10S530/81; Y10S530/812; Y10S530/815; Y10S530/816; Y10S530/817
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
192 words
A method of immobilizing proteinaceous substances comprising the steps of providing an insoluble microporous member, having at least a pair of opposed surfaces and a predetermined thickness said microporous member comprising a polymeric resinous binder having finely divided filler particles dispersed throughout said binder and a network of substantially interconnected pores formed therein, said bores being formed within said resinous binder, between said filler particles and said resinous binder, and between neighboring filler particles, said dispersed filler particles being present in said microporous member in an amount by weight of at least about 25%, the size distribution of said pores varying non-uniformly across each of said surfaces and across said predetermined thickness through the range of about 0.01 micron to about 100 microns as determined porosimetrically by the Mercury Intrusion Method, and bonding a proteinaceous substance to the surface of at least some of said plurality of dispersed filler particles, said microporous member being pervious to the flow of a fluid through at least one of said surfaces wherein at least some of said filler particles to which said proteinaceous substance is bound is adapted to come into contact with such fluid.
Claim 2
Independent Claim
192 words
A method of immobilizing proteinaceous substances comprising the steps of providing an insoluble microporous member, having at least a pair of opposed surfaces and a predetermined thickness said microporous member comprising a polymeric resinous binder having finely divided filler particles dispersed throughout said binder and a network of substantially interconnected pores formed therein, said bores being formed within said resinous binder, between said filler particles and said resinous binder, and between neighboring filler particles, said dispersed filler particles being present in said microporous member in an amount by weight of at least about 25%, the size distribution of said pores varying non-uniformly across each of said surfaces and across said predetermined thickness through the range of about 0.01 micron to about 100 microns as determined porosimetrically by the Mercury Intrusion Method, and bonding a proteinaceous substance to the surface of at least some of said plurality of dispersed filler particles, said microporous member being pervious to the flow of a fluid through at least one of said surfaces wherein at least some of said filler particles to which said proteinaceous substance is bound is adapted to come into contact with such fluid.
Claim 3
Dependent Claim
14 words
The method of claim 1 wherein said proteinaceous substance is a catalytically active enzyme.
Claim 4
Dependent Claim
70 words
The method of claim 2 wherein said catalytically active enzyme is chemically bound to said surface of said filler particles by treating said support member with an intermediate coupling agent to form organic functional groups covalently bonded to said surface, and said treated support member is exposed to a solution including said enzyme to convalently bond said enzyme to said organic functional groups on the surface of said filler particles.
Claim 5
Dependent Claim
24 words
The method of claim 3 wherein said treated support member is exposed to a cross-linking agent prior to being exposed to said enzyme solution.
Claim 6
Dependent Claim
21 words
The method of claim 3 wherein said treated support member is exposed simultaneously to said enzyme solution and a cross-linking agent.
Claim 7
Dependent Claim
13 words
The method of claim 3 wherein said intermediate coupling agent is an organosilane.
Claim 8
Dependent Claim
10 words
The method of claim 6 wherein said organosilane is gamma-aminopropyltriethoxysilane.
Claim 9
Dependent Claim
69 words
The method of claim 2 wherein said catalytically active enzyme is chemically bound to said surface of said filler particles by treating said support member with an intermediate coupling agent to form organic functional groups chemiadsorbed to said surface, and said treated support member is exposed to a solution including said enzyme to covalently bond said enzyme to said organic functional groups on the surface of said filler particles.
Claim 10
Dependent Claim
24 words
The method of claim 8 wherein said treated support member is exposed to a cross-linking agent prior to being exposed to said enzyme solution.
Claim 11
Dependent Claim
21 words
The method of claim 8 wherein said treated support member is exposed simultaneously to said enzyme solution and a cross-linking agent.
Claim 12
Dependent Claim
13 words
The method of claim 8 wherein said intermediate coupling agent is a polyelectrolyte.
Claim 13
Dependent Claim
10 words
The method of claim 11 wherein said polyelectrolyte is polyethelenimine.
Claim 14
Dependent Claim
18 words
The method of claim 4 wherein said intermediate coupling agent is gamma-aminopropyltriethoxysilane and said cross-linking agent is glutaraldehyde.
Claim 15
Dependent Claim
18 words
The method of claim 5 wherein said intermediate coupling agent is gamma-aminopropyltriethoxysilane and said cross-linking agent is glutaraldehyde.
Claim 16
Dependent Claim
18 words
The method of claim 9 wherein said intermediate coupling agent is polyethelenimine and said cross-linking agent is glutaraldehyde.
Claim 17
Dependent Claim
18 words
The method of claim 10 wherein said intermediate coupling agent is polyethelenimine and said cross-linking agent is glutaraldehyde.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1982-09-24 | 1983-03-26 | 1983-09-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1986-09-24 | 1987-03-26 | 1987-09-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1990-09-24 | 1991-03-26 | 1991-09-25 | $7700.00 | Window Closed / Lapsed |