Patent US4181649A successfully refreshed from Google Patents.
US4181649A
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Method of separating peptides
Assignee: Tosoh Corp
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Filing: 1978-04-26
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Granted: 1980-01-01
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Expires: 1998-04-26
Abstract
Abstract Peptides are separated by passing a solution of peptides and an eluent through a column containing a crosslinked hard type starch gel whereby peptides and/or other components are non-fractionally or fractionally separated.
Inventors
- 👤 Susumu Matsushita
- 👤 Tetsuo Ikushige
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 18:11
CPC Classifications
B; B01; B01J; B01J20/00; B01J20/281; B01J20/291; B01J2220/00; B01J2220/50; B01J2220/54
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
43 words
A method of separating peptides by gel permeation chromatography which comprises passing a solution of peptides and an eluent through a column containing a cross-linked hard type starch gel at a pressure of at least 1 kg/cm 2 whereby said peptides are separated.
Claim 2
Independent Claim
43 words
A method of separating peptides by gel permeation chromatography which comprises passing a solution of peptides and an eluent through a column containing a cross-linked hard type starch gel at a pressure of at least 1 kg/cm 2 whereby said peptides are separated.
Claim 3
Dependent Claim
48 words
A method according to claim 1 wherein the crosslinked hard type starch gel has a mechanical strength being durable to the pressure of at least 200 Kg/cm 2 and has an average particle diameter of 5 to 177μ and an average pore diameter of 13 to 1250 A.
Claim 4
Dependent Claim
29 words
A method according to claim 1 or 2 wherein the eluent is an aqueous solution of a salt, an acid or a base having pH of 1 to 12.
Claim 5
Dependent Claim
15 words
The method according to claim 2 wherein said average pore diameter is 5 to 25μ.
Claim 6
Dependent Claim
19 words
A method according to claim 1 wherein the eluent is a mixed medium of water and a non-aqueous medium.
Claim 7
Dependent Claim
30 words
A method according to claim 5 wherein said non-aqueous medium comprises at least one compound selected from the group consisting of C 1 -C 4 alcohols, tetrahydrofuran, acetonitrile and pyridine.
Claim 8
Dependent Claim
12 words
A method according to claim 1 wherein said peptides are fractionally separated.
Claim 9
Dependent Claim
12 words
A method according to claim 1 wherein said peptides are separated non-fractionally.
Claim 10
Dependent Claim
37 words
A method according to claim 1 wherein said cross-linked starched gel is cross-linked with an agent selected from the group consisting of divinyl benzene, 1,5-hexadiene-3-in, hexatriene, divinyl ether, divinyl sulfone, allyl phthalate, 2,6-diacryl phenol and diallyl carbinol.
Claim 11
Dependent Claim
12 words
The method according to claim 9 wherein said agent is divinyl sulfone.
Claim 12
Dependent Claim
17 words
A method according to claim 1 wherein said eluent has a pH of from 2 to 11.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1982-12-31 | 1983-07-02 | 1984-01-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1986-12-31 | 1987-07-02 | 1988-01-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1990-12-31 | 1991-07-02 | 1992-01-01 | $7700.00 | Window Closed / Lapsed |