Patent US4190645A successfully refreshed from Google Patents.
US4190645A
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Rotavirus inner capsid subunit preparations
Assignee: SmithKline Beecham Corp
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Filing: 1978-08-30
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Granted: 1980-02-26
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Expires: 1998-08-30
Abstract
Abstract A preparation of rotavirus inner capsid antigenic subunits which is substantially free from complete virus particles and fragments thereof. The subunits have a sedimentation coefficient of 17.5S, and a molecular weight of >2×10 5 and >2×10 6 . Such a preparation can be prepared by suspending a sample of faecal material obtained from a mammal infected with rotavirus, incubating the suspension followed by the removal and concentration of the subunits from the suspension by centrifugation and/or filtration. Such a preparation is useful in several immunological tests and for the preparation of antisera also for use in tests.
Inventors
- 👤 June D. Almeida
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 07:58
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/005; A; A61; A61K; A61K39/00; C12; C12N; C12N2720/00; C12N2720/00011; C12N2720/12011; C12N2720/12311; C12N2720/12322
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
36 words
A rotavirus inner capsid antigenic subunit preparation substantially free from complete virus particles and fragments thereof, wherein the subunits have a sedimentation coefficient of 17.5S, and a molecular weight of >2×10 5 and <2×10 6 .
Claim 2
Dependent Claim
36 words
A rotavirus inner capsid antigenic subunit preparation substantially free from complete virus particles and fragments thereof, wherein the subunits have a sedimentation coefficient of 17.5S, and a molecular weight of >2×10 5 and <2×10 6 .
Claim 3
Dependent Claim
34 words
A preparation as claimed in claim 1 wherein the subunits are circular in shape, when viewed in the electron microscope, with a diameter of 50 A and a 15 A hole in the centre.
Claim 4
Dependent Claim
57 words
A method for preparing a rotavirus inner capsid subunit preparation as claimed in claim 1 or claim 2 comprising suspending a sample of faecal material obtained from a mammal infected with rotavirus, incubating the suspension at 30° to 45° C. for at least 20 minutes, removing and concentrating the subunits from the suspension by centrifugation and/or filtration.
Claim 5
Dependent Claim
24 words
A method as claimed in claim 3 wherein the faecal material is suspended in a buffer at a pH of from 5 to 9.
Claim 6
Dependent Claim
20 words
A method as claimed in either claim 3 or claim 4 wherein the suspension is incubated at about 37° C.
Claim 7
Independent Claim
40 words
A method as claimed in any one of claims 3, 4 or 5 wherein the subunits are removed from the suspension by centrifugation at a speed sufficient to sediment whole virus particles and fragments, but insufficient to sediment the subunits.
Claim 8
Independent Claim
25 words
A method as claimed in any one of claims 3, 4, 5 or 6 wherein the initial faecal suspension is incubated with a proteolytic enzyme.
Claim 9
Dependent Claim
15 words
A method as claimed in claim 7 wherein the proteolytic enzyme is trypsin or papain.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-02-25 | 1983-08-27 | 1984-02-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-02-25 | 1987-08-27 | 1988-02-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-02-25 | 1991-08-27 | 1992-02-26 | $7700.00 | Window Closed / Lapsed |